Hepatitis C Virus Genotyping by Polymerase Chain Reaction and DNA Enzyme Immunoassay among Saudi Patients in the Western Province, Saudi Arabia

Osoba, Abimbola O.; Ibrahim, Mohamed N.; Abdelaal, M.; Al-Mowallad, A.; Al-Shareef, Bakheet A.; Hussein, Batool A. Hussein

doi:10.5144/0256-4947.2000.394

Cited by 18 publications

(14 citation statements)

References 16 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Genotype 3a was the predominant genotype in this study, which is in line with the findings of studies conducted in India, Pakistan and Nepal (13,14). However, these findings are inconsistent with the results of studies in Saudi Arabia (15), Kuwait (16), France (17), North American countries (18) and South Korea (19). This difference could be due to high prevalence of intravenous drug abuse in the present study (48.33%) because numerous molecular epidemiological studies suggest an association between genotype 3a transmission and history of intravenous drug abuse (13,16,20).…”

Section: Discussionsupporting

confidence: 76%

Epidemiologic Study of Hepatitis C Virus Transmission in Lorestan Province (2011-14)

Nazer¹,

Obeidavi²,

Beiranvand

et al. 2016

mljgoums

View full text Add to dashboard Cite

Background and Objective: This study was conducted to evaluate epidemiology of risk factors for hepatitis C virus (HCV) transmission, and determine the association of its genotypes with viral load and response to treatment in patients referred to the Infectious Disease Clinic of Khorramabad.Methods: The study included patients infected with hepatitis C referred to the Infectious Disease Clinic of Khorramabad during 2011-14. Those who met the inclusion criteria were referred to counseling centers for risky behaviors. After collecting demographic and epidemiologic data on the modes of transmission, blood samples were collected for more accurate testing. The erum of patients with hepatitis C was evaluated in terms of HCV genotype and viral load using PCR.Results: Among 120 patients studied, 102 (85%) were male and 18 (15%) were female. The most prevalent genotypes were 3a (63.8%), 1a (25%), 1a/1b (5.2%) and 2 (1.7%). Intravenous drug abuse was the main route of transmission of hepatitis C in the study. The genotypes 3a and 1a had a clear correlation with intravenous drug abuse and history of blood transfusion (P<0.05). It was also found that the patients with genotype 3a show a better initial response to treatment with interferon.Conclusion: The study shows that intravenous drug abuse is the main route of transmission, which highlights the need for intervention and education in this group of patients to prevent disease transmission.

show abstract

Section: Discussionsupporting

confidence: 76%

Epidemiologic Study of Hepatitis C Virus Transmission in Lorestan Province (2011-14)

Nazer¹,

Obeidavi²,

Beiranvand

et al. 2016

mljgoums

View full text Add to dashboard Cite

show abstract

“…Genotype 4, followed by genotypes1a and 1b, were found to be the most common genotypes in Saudi patients, whereas genotypes 2, 3, 5 and 6 were rarely found [5], [6], [7], [8]. In the current study, 81/83 strains were found to be classified as genotype 4 while only 2/83 strains were classified as genotype 1 (Figure 2).…”

Section: Resultsmentioning

confidence: 44%

“…Genotype 4 (HCV-4) is common in the Middle East and in Africa, where it is responsible for more than 80% of HCV infections [4]. In Saudi Arabia, HCV-4 was the most prevalent genotype followed by HCV-1 whereas genotypes 2, 3, 5 and 6 were rarely reported [5], [6], [7], [8].…”

Section: Introductionmentioning

confidence: 99%

HCV Infection among Saudi Population: High Prevalence of Genotype 4 and Increased Viral Clearance Rate

et al. 2012

View full text Add to dashboard Cite

HCV is a major etiological agent of liver disease with a high rate of chronic evolution. The virus possesses 6 genotypes with many subtypes. The rate of spontaneous clearance among HCV infected individuals denotes a genetic determinant factor. The current study was designed in order to estimate the rate of HCV infection and ratio of virus clearance among a group of infected patients in Saudi Arabia from 2008 to 2011. It was additionally designed to determine the genotypes of the HCV in persistently infected patients. HCV seroprevalence was conducted on a total of 15,323 individuals. Seropositive individuals were tested by Cobas AmpliPrep/Cobas TaqMan HCV assay to determine the ratio of persistently infected patients to those who showed spontaneous viral clearance. HCV genotyping on random samples from persistently infected patients were conducted based on the differences in the 5′untranslated region (5′UTR). Anti-HCV antibodies were detected in 7.3% of the totally examined sera. A high percentage of the HCV infected individuals experienced virus clearance (48.4%). HCV genotyping revealed the presence of genotypes 1 and 4, the latter represented 97.6% of the tested strains. Evidences of the widespread of the HCV genotype 4 and a high rate of HCV virus clearance were found in Saudi Arabia.

show abstract

“…Genotype 3a is the second most common genotype in Europe after genotype 1 4,6 . Genotype 4 is prevalent in the Middle East [12][13][14] and in Central Africa [15][16][17] , while genotype 5 is common in South Africa 1,18 , and genotype 6 is found mainly in South Asia [19][20][21] . Genotype 7 has been found in Vietnamese patients with chronic hepatitis C, although some authors have advocated that it should be reclassifi ed as a subtype of genotype 6 22 .…”

Section: Introductionmentioning

confidence: 99%

Do differences exist between chronic hepatitis C genotypes 2 and 3?

Melo

Ferraz

Perez

et al. 2014

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Introduction: Six genotypes of the hepatitis C virus (HCV) have been identifi ed thus far, and their distribution is well defi ned. Genotype 1, which is the most prevalent worldwide, is always compared to genotypes 2 and 3, particularly in terms of treatment response. However, little is known about the differences between genotypes 2 and 3 because these genotypes are analyzed together in most studies. Therefore, the aim of this study was to evaluate differences in the clinical, epidemiological, laboratory, and histological parameters between HCV-2 and HCV-3. Methods: Patients with chronic hepatitis C infected with genotypes 2 and 3 were studied retrospectively and compared according to clinical, laboratory, and histological aspects. Hepatitis C virusribonucleic acid (HCV-RNA) was analyzed quantitatively by TaqMan® real-time PCR, and the HCV genotype was determined by sequencing the 5'-untranslated region. Results: A total of 306 patients with chronic HCV-2 (n=50) and HCV-3 (n = 256) were studied. Subtype 2b (n=17/50) and subtype 3a (n=244/256) were the most prevalent among patients infected with HCV-2 and HCV-3, respectively. The mean age was 47 ± 10 years, and there was a predominance of men in the group studied (61%). Comparative analysis between HCV-2 and HCV-3 showed a younger age (p=0.002), less prevalence of arterial hypertension (p=0.03), higher serum albumin levels (p=0.01), more advanced stage of liver fi brosis (p=0.03), and higher frequency of steatosis in patients with HCV-3 (p=0.001). After multivariate regression analysis, all the variables, except serum albumin, remained as variables associated with HCV-3 in the fi nal model. Conclusions: Clinical and histological differences exist between HCV-2 and HVC-3, which suggests the need for separate analyses of these genotypes.

show abstract

Hepatitis C Virus Genotyping by Polymerase Chain Reaction and DNA Enzyme Immunoassay among Saudi Patients in the Western Province, Saudi Arabia

Cited by 18 publications

References 16 publications

Epidemiologic Study of Hepatitis C Virus Transmission in Lorestan Province (2011-14)

Epidemiologic Study of Hepatitis C Virus Transmission in Lorestan Province (2011-14)

HCV Infection among Saudi Population: High Prevalence of Genotype 4 and Increased Viral Clearance Rate

Do differences exist between chronic hepatitis C genotypes 2 and 3?

Contact Info

Product

Resources

About