Hepatitis C virus genotypes in hemophiliacs in the state of Minas Gerais, Brazil

Oliveira, Guilherme; Carmo, Ricardo Andrade; Rocha, Miriam Oliveira e; Silva, M.O.; Lima, Alison T. M.; Guimarães, Mark Drew Crosland; Corrêa-Oliveira, R

doi:10.1046/j.1537-2995.1999.39111194.x

Cited by 16 publications

(23 citation statements)

References 29 publications

(37 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Previous data from Minas Gerais were obtained for hemophiliacs (26) or patients under hemodialysis (23). We confirmed the predominance of genotype 1 in this state, but we also detected genotypes 2 and 3.…”

Section: Discussionsupporting

confidence: 83%

Geographic distribution of hepatitis C virus genotypes in Brazil

Campiotto

Pinho

Carrilho

et al. 2005

Braz J Med Biol Res

174

102

View full text Add to dashboard Cite

Brazil is a country of continental dimension with a population of different ethnic backgrounds. Thus, a wide variation in the frequencies of hepatitis C virus (HCV) genotypes is expected to occur. To address this point, 1,688 sequential samples from chronic HCV patients were analyzed. HCV-RNA was amplified by the RT-PCR from blood samples collected from 1995 to 2000 at different laboratories located in different cities from all Brazilian States. Samples were collected in tubes containing a gel separator, centrifuged in the site of collection and sent by express mail in a refrigerated container to Laboratório Bioquímico Jardim Paulista, São Paulo, SP, Brazil. HCV-RNA was extracted from serum and submitted to RT and nested PCR using standard procedures. Nested PCR products were submitted to cycle sequencing reactions without prior purification. Sequences were analyzed for genotype determination and the following frequencies were found: 64.9% (1,095) for genotype 1, 4.6% (78) for genotype 2, 30.2% (510) for genotype 3, 0.2% (3) for genotype 4, and 0.1% (2) for genotype 5. The frequencies of HCV genotypes were statistically different among Brazilian regions (P = 0.00017). In all regions, genotype 1 was the most frequent (51.7 to 74.1%), reaching the highest value in the North; genotype 2 was more prevalent in the Center-West region (11.4%), especially in Mato Grosso State (25.8%), while genotype 3 was more common in the South (43.2%). Genotypes 4 and 5 were rarely found and only in the Southeast, in São Paulo State. The present data indicate the need for careful epidemiological surveys throughout Brazil since knowing the frequency and distribution of the genotypes would provide key information for understanding the spread of HCV.

show abstract

Section: Discussionsupporting

confidence: 83%

Geographic distribution of hepatitis C virus genotypes in Brazil

Campiotto

Pinho

Carrilho

et al. 2005

Braz J Med Biol Res

174

102

View full text Add to dashboard Cite

show abstract

“…In our study, the HCV-RNA frequency in plasma samples from anti-HCV-positive patients (82.5%) was similar to that reported in other studies in Brazil [42,44]. The predominance of genotype 1 (68.75% of all HCV-RNA positive cases) also concurs with published data from other studies conducted in Brazil [42,[44][45][46].…”

Section: Discussionsupporting

confidence: 91%

“…The predominance of genotype 1 (68.75% of all HCV-RNA positive cases) also concurs with published data from other studies conducted in Brazil [42,[44][45][46].…”

Section: Discussionsupporting

confidence: 90%

Detection of hepatitis C virus RNA in saliva samples from patients with seric anti-HCV antibodies

Gonçalves¹,

Cunha²,

Busek³

et al. 2005

Braz J Infect Dis

View full text Add to dashboard Cite

We examined the frequency of HCV-RNA in saliva samples from anti-HCV positive patients. Both plasma and saliva samples from 39 HCV patients (13 with normal liver enzymes, 19 with abnormal liver enzymes and 13 with cirrhosis) were investigated. Stimulated saliva and fresh plasma were centrifuged (900 x g,10 min) and stored at -70 o C, after the addition of guanidine isothiocyanate RNA extraction buffer. HCV-RNA was detected by RT-nested-PCR (amplification of HCV-cDNA for two rounds, using HCV primers 939/209 and 940/211). HCV genotyping was carried out by RFLP (using Mva I and Hinf 1 or Hae III and Rsa I restriction enzymes). Thirty-two out of 39 (82%; 95% CI=70-94%) anti-HCV-positive patients had HCV-RNA in plasma samples. Eight out of 39 (20.5%; 95% CI=6.6-34.4%) had HCV-RNA in the saliva. The HCV genotype in saliva samples from these patients matched the genotype found for plasma HCV-RNA. No significant correlation between the presence of HCV and either age, gender, HCV genotype or any risk factor for HCV infection was found. The observed prevalence (20.5% of anti HCV positive patients or 25% of the patients with HCV-RNA in plasma) was lower than that previously reported from other countries. The low frequency of HCV-RNA in saliva samples observed in our study may be due to the use of cell-free saliva. Other authors reporting higher frequencies of HCV-RNA in saliva used whole saliva, without centrifugation.

show abstract

“…The use of clotting factor concentrates became common only after 1997, more than a decade after the introduction of virus-inactivation techniques. As data concerning HCV infection in Brazilian hemophiliacs are still rare (Rocha et al 1994, Oliveira et al 1999, Martins et al 2000, we sought to assess an overall HCV prevalence of infection (anti-HCV and RNA-HCV) in hemophiliacs in Central Brazil. In addition, we studied risk factors associated with HCV infection in this population.…”

mentioning

confidence: 99%