Hepatitis B virus genotypes and precore/core-promoter mutations in Tunisian patients with chronic hepatitis B virus infection

Ayed, K.; Gorgi, Yousr; Ayed-Jendoubi, Saloua; Aouadi, Houda; Sfar, Imen; Najjar, Tawfik; Abdallah, T. Ben

doi:10.1016/j.jinf.2006.05.013

Cited by 26 publications

(41 citation statements)

References 62 publications

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“…Genotyping revealed that all 50 HBV-DNA-positive samples were of genotype D. RFLP was chosen for genotyping of HBV samples in our study as it is a simple method to perform and requires only a PCR prior to its application [3]. Our data are similar to both local and international studies performed on HBV genotypes prevalent in Egypt [19,20,21] and other Mediterranean and Middle Eastern countries [29,30]. …”

Section: Discussionsupporting

confidence: 64%

Hepatitis B Virus Genotype D Predominates HBsAg-Positive Egyptian Blood Donors and Is Mainly Associated with a Negative HBeAg Serostatus

Habil¹,

Mahdi

Abdelwahab

et al. 2013

Intervirology

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Objectives: Hepatitis B virus (HBV) infection is a global health burden. In this regard, Egypt has an intermediate HBV seroprevalence. HBV is classified into ten different genotypes (A-J) with different geographic distributions. Genotype D is the most prevalent in the Middle East. Limited data are available about HBV genotyping among Egyptian blood donors, particularly in Upper Egypt. We examined the seroprevalence of HBV among 12,000 blood donors attending the blood transfusion services center in Minia Governorate, Upper Egypt. Methods: HBsAg and HBeAg were examined by ELISA while HBV-DNA was examined by PCR. HBV genotyping was conducted by restriction fragment length polymorphism. Results: HBsAg was detected in 237 donors (1.98%). The HBV-DNA of 50 donors with the highest HBsAg OD was examined for the HBV genotype. All 50 DNA-positive samples were of genotype D. 82% of the DNA-positive donors were males, coinciding with their representation in the cohort. ALT levels were normal in 88% of genotyped subjects, while 84% of them were HBeAg negative. Conclusion: Taken together, these data indicate that HBV genotype D is the predominant genotype among HBsAg-positive blood donors in Upper Egypt and was in >80% of the subjects associated with a negative HBeAg serostatus.

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Section: Discussionsupporting

confidence: 64%

Hepatitis B Virus Genotype D Predominates HBsAg-Positive Egyptian Blood Donors and Is Mainly Associated with a Negative HBeAg Serostatus

Habil¹,

Mahdi

Abdelwahab

et al. 2013

Intervirology

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“…The serum ALT was maintained within the normal reference range in 83.5% and 87% of patients at one year and three to four years of follow-up, respectively; previous data for CHB patients maintaining normal ALT during follow-up showed a benign course for the disease [2][3][4]29]. Ayed et al showed that HBeAg-positive patients had higher serum ALT levels compared to HBeAg -negative patients [30]; however, in our cohort, the ALT levels were not different between the two groups. This difference in the results of the two studies may be explained by the fact that not all HBeAg-negative patients are chronic carriers; instead, some of them have active HBeAg-negative CHB [2,3] .…”

Section: Discussionmentioning

confidence: 88%

Chronic hepatitis B infection in a hepatology clinic at a university hospital in Jeddah

Fallatah

Akbar

2010

J Infect Dev Ctries

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Introduction: The outcome of chronic hepatitis B (CHB) infection in a cohort of CHB patients at the hepatology clinics of King Abdul Aziz University Hospital in Jeddah was studied. Methodology: The results of a complete blood count, prothrombin time, liver function test and hepatitis B virus polymerase chain reaction (HBV-PCR) performed over the previous two to five years of follow-up were reviewed. Results of abdominal ultrasound performed within the last year and the data on the treatment type, duration and resistance were also obtained. Results: The majority of the 109 patients studied were Hepatitis B e antigen (HBeAg-negative; 87.2%). Male patients had higher serum ALT values compared to females at follow-up. HBeAg-positive patients had higher HBV-PCR levels at diagnosis compared to HBeAgnegative patients. Patients below 40 years of age had higher HBV-PCR compared to those above 40 years. Ultrasound showed liver cirrhosis in 11% of patients. Cirrhotic patients had higher GGT levels compared to non cirrhotic patients. Drug resistance developed in 25% of the 20 Lamivudine-treated patients. The mean duration of treatment was 2.5 ± .47 years. Conclusion: CHB was predominantly HBeAg negative, with a benign long-term outcome in most patients. Therapy may need to be individualized for patients with high risk of progression.

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“…Ancak bizim çalışmamızda HBeAg pozitif hastaların %20'sinde prekor mutasyonu tespit edilmesine rağmen HBeAg kaybolmamış-tır. Benzer sonuçlar başka araştırmacılar tarafından da bildirilmiştir 17,19,23,28 . PC G1896A mutasyonu ile HBeAg pozitifl iği arasındaki bu uyumsuzluk, HBeAg serokonversiyon sü-recinde vahşi tip HBeAg salgılayan virus popülasyonları ile karışık enfeksiyon oluşumu ile açıklanabilmekle birlikte, enfeksiyonun seyri sırasında HBeAg'nin temizlenmesi beklenmektedir.…”

Section: Mutasyon Tipleri Negatif N (%) Negatif N (%) Toplam N (%)unclassified

“…Ancak bazı değişik bölgelerde BCP nt. 1762/1764 mutasyonlarının, HBeAg negatif (%36-71.4) hastalarda pozitifl ere (%11-42.5) oranla daha yaygın görüldüğü rapor edilmiştir 21,23,24 . Bu sonuçlar, HBeAg negatif ve pozitif hastalar arasında, BCP gen mutasyonlarının coğ-rafi olarak farklı dağılıma sahip olabileceğini, bu farklılığa viral faktörlerin (predominant olarak görülen HBV genotipi gibi) ve konak faktörlerinin (HLA tipi gibi) etki edebileceğini göstermektedir.…”

Section: Mutasyon Tipleri Negatif N (%) Negatif N (%) Toplam N (%)unclassified

“…HBV genotiplerinin dünya genelinde dağılımındaki heterojenite, farklı popülasyon-larda HBV mutasyonlarının prevalansındaki farklılıktan da sorumlu tutulmaktadır 12 . HBV BCP/PC mutasyonları ile ilgili çalışmalar, genotip yönünden heterojen hasta popülasyon-larında gerçekleştirildiğinden verilerin değerlendirilmesi zorlaşmaktadır 23,24 . Bu sebeple yaptığımız çalışmanın, tek bir genotip ile yansıtılan homojen hasta popülasyonunda değerlendirilmiş olması oldukça önemlidir.…”

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Characterization of Basal Core Promoter/Precore Gene Mutations in Chronically Infected Patients with Hepatitis B Virus Genotype D in Mersin Province, Turkey

Tezcan¹,

Ülger²,

Üçbilek³

et al. 2015

Mikrobiyol Bul

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Hepatitis B virus genotypes and precore/core-promoter mutations in Tunisian patients with chronic hepatitis B virus infection

Cited by 26 publications

References 62 publications

Hepatitis B Virus Genotype D Predominates HBsAg-Positive Egyptian Blood Donors and Is Mainly Associated with a Negative HBeAg Serostatus

Hepatitis B Virus Genotype D Predominates HBsAg-Positive Egyptian Blood Donors and Is Mainly Associated with a Negative HBeAg Serostatus

Chronic hepatitis B infection in a hepatology clinic at a university hospital in Jeddah

Characterization of Basal Core Promoter/Precore Gene Mutations in Chronically Infected Patients with Hepatitis B Virus Genotype D in Mersin Province, Turkey

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