Hepatitis B Surface Antigen Should Not Be the Only Sought Marker to Distinguish Blood Donors towards Hepatitis B Virus Infection in High Prevalence Area

Abstract

Since its discovery by Blumberg in 1965, the hepatitis B surface antigen (HBsAg) is used as the fingerprint of hepatitis B infection. Occult hepatitis B infection (OBI) is defined by a viral replication (DNA detectable) in the absence of HBsAg. Burkina Faso is a high endemic area where the prevalence is higher than 14%. At the National Center for Blood Transfusion (NCBT) of Ouagadougou, HBsAg is the only sought marker used to distinguish donors towards Hepatitis B Virus (HBV). Acceptation of blood donation is based specifically on the absence of HBsAg, which exposes to the risk of HBV transmission during transfusion. The goal of this study is to evaluate this risk by determining the prevalence of OBI in blood donors. Patients and Methods: It was a five-month prospective study on blood donations collected from January to May 2016. The HBc antibody has been sought in the serums of negative HBsAg donors. The measure of B DNA by Real Time PCR (polymerase chain reaction) and that of antibodies anti-HBs have been proposed to anti-HBc positive donors. Abdominal ultrasound, the transaminases, prothrombin level, alphafeto-proteins, hepatic fibrosis have been proposed to donors who were detectable for the DNA. Sociodemographic parameters have been collected. The test costs were borne by donors who were recalled by phone to adhere to the study. Results: Among 1980 negative donors HBsAg, 872 (44%) were positive for anti-HBc. 160 on 872 donors were received for consultation, among which 76 (76/160) were able to realise DNA which was detectable in 25 donors on 76, thus a prevalence of 32.8%. The mean value of DNA was 953 IU/ml. Physical examination and hepatic ultrasounds were normal except a case where hepatic steatosis was found. The biologic standard hepatic results were in normal range. None of the patient was able to realise hepatic fibrosis evaluation. A case of co-infection HIV/OBI was noted. Conclusion: This study shows that in BurHow to cite this paper: Somda, K.S., Sermé, A.K., Coulibaly, A., Cissé, K., Sawadogo, A., Sombié, A.R. and Bougouma, A. 363kina, almost half of blood bags transfused are anti-HBc positive and around one third (32.8%) probably have HBV DNA. This poses a potential risk of contamination for non-immunized recipient. It is thus important that, in addition to HBsAg, AntiHBc should be systematically sought in order to minimize the risk.

Occult Hepatitis B Virus Infection and Associated Genotypes Among Hbsag-Negative Subjects in Burkina Faso

“…The latter is lower than that reported among HIV-positive patients from Ivory Coast in 2010 and from Sudan in 2014, and among blood donors from Burkina Faso in 2016; 10%, 15%, and 32.8% respectively. 17 , 21 , 22 Nevertheless, our prevalence was similar to that of 6.25% reported among Egyptian blood donors in 2010. 19 However, it was higher than 0.5% reported among regular blood donors in Southeast Nigeria.…”

“…This prevalence is lower than 44.0% reported in HBsAg-negative blood donors in Burkina Faso. 17 However, it is higher than 7.8% and 16.6% reported in HBsAg-negative blood donors in Egypt. 19 , 20 These differences could be explained by the size and type of study population but also by endemicity for HBV.…”

“…However, a recent study reported a prevalence of 32.8 % (25/76) of OBI among blood donors of Ouagadougou. 17 Thus, this study aimed to determine the prevalence of OBI among HBsAg negative subjects and characterize the associated genotypes.…”

Impact of Multiplex PCR in Reducing the Risk of Residual Transfusion-Transmitted Human Immunodeficiency and Hepatitis B and C Viruses in Burkina Faso

“…Added to this is the high prevalence of occult hepatitis B reported in several studies in Burkina Faso. 28 , 29 Occult hepatitis B is characterized by the presence of HBV DNA in the serum of a patient who is screened for HBsAg by the usual serological tests. 30 Although our study did not allow us to detect residual cases associated with HIV and HCV, the number of donations detected positive HBV by PCR confirms that PCR is more sensitive than ELISA.…”

Prevalence of occult hepatitis B virus infection among blood donors in Ouagadougou, Burkina Faso

“…Occult HBV infection is transmissible through blood transfusion in the event of biological qualification of donations without the use of the polymerase chain reaction (PCR) assay, and through organ transplants. The prevalence of occult HBV infection in Burkina Faso varies according to studies; Birama et al, (10) reported a prevalence rate of 7.3% in 2018 in the population of Ouagadougou while Somda et al, (11) reported a seroprevalence rate of 32.8% among blood donors in 2016.…”