Impact of Multiplex PCR in Reducing the Risk of Residual Transfusion-Transmitted Human Immunodeficiency and Hepatitis B and C Viruses in Burkina Faso

Yooda, Arzouma Paul; Soubeïga, Serge Théophile; Nebie, Kompingnin Yacouba; Diarra, Birama; Sawadogo, S.; Ouattara, Abdoul Karim; Obiri‐Yeboah, Dorcas; Yonli, Albert Théophane; Tao, Issoufou; Sorgho, Pegdwendé Abel; Dahourou, Honorine; Simporé, Jacques

doi:10.4084/mjhid.2018.041

Cited by 14 publications

(14 citation statements)

References 27 publications

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“…The prevalence of anti-HBc antibody of 13% in this study is lower than the rate of 18.4% reported by Yooda et al, (8) in 2017 and 44.0% by Somda et al, (11) in 2016 among blood donors in Burkina Faso. How-ever, it is higher than the 7.8% rate reported among blood donors in Egypt by Antar et al, (16) in 2010.…”

Section: Discussioncontrasting

confidence: 73%

“…This finding could probably be linked to the dynamism and ability of the young population to donate blood. It is all fairer to say that the best blood donors in Burkina Faso are pupils and students (8).…”

Section: Discussionmentioning

confidence: 99%

“…In Burkina Faso, HBV infection is the major cause of hepatic cirrhosis and liver cancer (3). The country is among those with high endemicity, where the carriage of hepatitis B virus surface antigen (HBsAg) is high in the general population, with a prevalence varying from 9% to 15% in 2018 (4-7), and seroprevalence rate of 7.28% among blood donors in 2017 (8).…”

Section: Introductionmentioning

confidence: 99%

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Prevalence of occult hepatitis B virus infection among blood donors in Ouagadougou, Burkina Faso

Ky-Ba¹,

Sanou²,

Ouédraogo³

et al. 2021

Af J Clin Exp Micro

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Background: In Burkina Faso, the polymerase chain reaction (PCR) assay is not routinely used in the biological qualification of blood donations and this constitutes a risk factor for the transmission of occult hepatitis B virus (HBV) infection during blood transfusion. The objective of this study is to determine the prevalence of occult B infection (OBI) among blood donors for the purposes of improved blood safety in Burkina Faso. Methodology: A descriptive cross-sectional study of 300 HBsAg negative blood donors was conducted in the city of Ouagadougou, Burkina Faso from April to October 2020. Anti-HBc antibody was determined using the BOSON® brand rapid tests. HBV DNA was detected in 75 selected donors by real-time PCR (rt PCR) using the 7500 Fast Real Time PCR assay technique. Results: Of the 300 HBsAg negative donors, 208 (69.3%) were males while 92 (30.7%) were females, with average age of 30.18 years. Anti-HBc antibody was detected in 39 cases (13%). Of the 75 donor samples tested by rt PCR, 3 (4%) were positive for HBV DNA (occult B infection); 2 of which were anti-HBc antibody positive (seropositive OBI) while 1 was anti-HBc antibody negative (seronegative OBI). Conclusion: Given the prevalence of OBI of 4% in this study and its consequences in blood recipients, it appears necessary that in addition to the classic serological markers of hepatitis B, to test for the presence of HBV DNA among blood donors in order to improve transfusion safety. Keywords: Prevalence, Occult B infection; Blood donors, Ouagadougou. French title: Prévalence de l'infection occulte par le virus de l'hépatite B chez les donneurs de sang à Ouagadougou, Burkina Faso Contexte: Au Burkina Faso, la polymérase chain réaction (PCR) n’est pas utilisée lors de la qualification biologique des dons et cela constitue un facteur de risque de transmission de l’Infection Occulte du virus B (VHB) lors des transfusions sanguines. L’objectif de cette étude était de déterminer la prévalence de l’infection occulte B chez les donneurs de sang en vue d’une meilleure sécurité transfusionnelle. Méthodologie: Une étude transversale prospective, réalisée d’avril à octobre 2020 dans la ville de Ouagadougou incluant 300 donneurs de sang AgHBs négatif. L’anticorps anti HBc a été déterminé par les tests rapides de marque BOSON®. L’ADN du VHB a été recherché chez 75 donneurs par PCR en temps réel (rt PCR) avec le 7500 Fast Real Time PCR. Résultats: Parmi les 300 donneurs AgHBs négatifs, 208 (69,3%) étaient des hommes et 92 (30,7%) des femmes. L’âge moyen était de 30,18 ans. La recherche de l’Ac anti-HBc était positive dans 39 cas (13 %). Parmi les 75 échantillons passés à la rt PCR, 3 (4%) étaient positifs pour l’ADN du VHB. Sur les 3 cas d’ADN VHB positifs, 2 (66,67%) étaient positifs Ac anti HBc et 1 (33,33%) Ac anti HBc négatif. Conclusion: Compte tenu de la prévalence de l’infection occulte B et ses conséquences chez les donneurs de sang et chez les receveurs, il devient nécessaire de rechercher, en plus des marqueurs sérologiques classiques de l’hépatite B, l’ADN VHB pour une meilleure sécurité transfusionnelle. Mots clés: Prévalence, Infection occulte de l’HBV, Donneurs de sang, Ouagadougou

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Section: Discussioncontrasting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prevalence of occult hepatitis B virus infection among blood donors in Ouagadougou, Burkina Faso

Ky-Ba¹,

Sanou²,

Ouédraogo³

et al. 2021

Af J Clin Exp Micro

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“…In Burkina Faso, studies5–7 have reported very high seroprevalence of these infections in both the general population and blood donors. In addition, a survey8 conducted in 2005 among blood donors who attended the blood bank of Yalgado Ouédraogo Teaching Hospital concluded that there was insufficient knowledge of blood donors about transfusion-transmitted infections (TTIs).…”

Section: Introductionmentioning

confidence: 99%

<p>Residual risk of HIV, HCV, and HBV transmission by blood transfusion between 2015 and 2017 at the Regional Blood Transfusion Center of Ouagadougou, Burkina Faso</p>

Yooda¹,

Sawadogo²,

Soubeïga³

et al. 2019

JBM

Self Cite

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In sub-Saharan Africa, the high endemicity of blood-borne infections is a serious threat to transfusion safety. In order to improve transfusion safety, Burkina Faso has undertaken in recent years a reorganization of its blood-transfusion system through the creation of a National Blood Transfusion Center, which is the only blood operator in the whole country. This study aimed to estimate the residual risk of transmission of HIV, hepatitis B virus (HBV), and hepatitis C virus (HCV) by blood transfusion at the Regional Blood Transfusion Center (RBTC) of Ouagadougou. Methods: This was a retrospective study conducted at the RBTC of Ouagadougou between 2015 and 2017. Prevalence of infectious markers was calculated for first-time donors and incidence rates calculated for repeat donors who had made at least two donations of blood over the study period. Residual risks were estimated for the three viruses (HIV, HBV, and HCV) by multiplying the incidence rate per 100,000 person-years by the respective durations of serological windows. Results: Between 2015 and 2017, of a total of 84,299 blood donors, 68,391 (81.13%) were first-time donors compared to 15,908 (18.87%) repeat donors. The seroprevalence of HBV (8.56%) was twice that of HCV (4.40%) and fourfold that of HIV (1.80%). Incidence rates were 1,215, 2,601, and 1,599 per 100,000 donations for HIV, HCV, and HBV, respectively. In contrast, the estimated residual risk for HCV (1 in 213 donations) was double that of HBV (1 in 408 donations) and four times that of HIV (1 in 1,366). Conclusion: The residual risk of transmission of these viruses by blood transfusion remains high in repeat donors. An effective donor-retention and education policy could help to reduce this residual risk.

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“…Sensitive molecular methods like multiplex real-time PCR in clinical practice can help the rapid diagnosis of viruses causing respiratory infection and reduce improper antibiotic therapy (32). Overall, several investigations have described that multiplex real-time PCR as a non-invasive method is faster and more sensitive than other standard methods such as serology and culture (33,34).…”

Section: Discussionmentioning

confidence: 99%

Detection of Common Respiratory Viruses in Patients with Acute Respiratory Infections Using Multiplex Real-Time RT-PCR

Neisi

Abbasi²,

Makvandi

et al. 2019

Jundishapur J Microbiol

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Background: Acute respiratory infection (ARI) is caused by human metapneumovirus (HMPV), respiratory syncytial virus type A and B (RSV-A, RSV-B), human parainfluenza viruses 1, 2, and 3 (HPIV-1, HPIV-2, and HPIV-3), influenza viruses A and B (IfV-A, IfV-B), and human coronaviruses (OC43/HKU1, NL63, 229E) worldwide. Objectives: This study was conducted to assess the causative agents of viral ARI among hospitalized adults by real-time PCR. Methods: Clinical nasopharyngeal swabs of 112 patients including 55 (49.1%) males and 57 (50.89%) females with ARI were analyzed using multiplex real-time RT-PCR. Results: Out of 112 specimens, 61 (54.46%) were positive including 10 (8.9%) for influenza H3N2, one (0.89%) for influenza B, 28 (25%) for RSV-A, 18 (16.07%) for HMPV-A, two (1.78%) for HPIV-1, and three (2.67%) for HPIV-3. Two (1.78%) specimens were positive for two agents, RSV-A/HMPV-A and RSV-A/HPIV-3. The distribution of viral infections was 30 among males (26.78%) and 31 (27.67%) among females (P = 0.862). High frequency of RSV-A infection (25%) and the low frequency of influenza B virus infection (0.89%) were detected among patients. The remaining 51 (45.53%) samples were negative for RSV-B, HMPV-B, IfV-A, HPIV2-4A-4B, and HCoVs (OC43/HKU1, NL63, 229E). Conclusions: The role of other viruses such as human adenoviruses rhinovirus/enterovirus (RV/EV), human bocavirus (HBoV), and human parechovirus (HpeV) was not investigated. Multiplex PCR can be used as a rapid test for the diagnosis of viruses causing acute respiratory infection, which results in decreased length of hospitalization.

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Impact of Multiplex PCR in Reducing the Risk of Residual Transfusion-Transmitted Human Immunodeficiency and Hepatitis B and C Viruses in Burkina Faso

Cited by 14 publications

References 27 publications

Prevalence of occult hepatitis B virus infection among blood donors in Ouagadougou, Burkina Faso

Prevalence of occult hepatitis B virus infection among blood donors in Ouagadougou, Burkina Faso

<p>Residual risk of HIV, HCV, and HBV transmission by blood transfusion between 2015 and 2017 at the Regional Blood Transfusion Center of Ouagadougou, Burkina Faso</p>

Detection of Common Respiratory Viruses in Patients with Acute Respiratory Infections Using Multiplex Real-Time RT-PCR

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