Hepatic α2u-globulin mRNA levels and diethylstilbestrol-associated testicular atrophy in rats

Takeyoshi, Masahiro; Anai, Shunji; Shinoda, Kazutoshi

doi:10.1016/s0890-6238(00)00090-3

Cited by 6 publications

(4 citation statements)

References 15 publications

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“…The expression of alpha-2u globulin (Spot 8) was also downregulated by TCE. This protein is expressed primarily in the mature male rat liver and excreted in the urine, and is absent from or only marginally detectable in female rats 19,20 . Expression of the alpha-2u globulin gene in male rat liver has been changed under oxidative stress to affect developmental and multihormonal regulation 21 .…”

Section: Resultsmentioning

confidence: 99%

“…The search program ProFound developed by The Rockefeller University (http:// 129.85. 19.192/profound_bin/WebProFound.exe) was used for protein identification by peptide mass fingerprinting. Spectra were calibrated using the trypsin autodigestion ion peaks at m/z 842.510 and 2211.1046 as internal standards.…”

Section: Enzymatic In-gel Digestion and Mass Spectrometrymentioning

confidence: 99%

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Proteomic analysis of rat liver proteins differentially induced by trichloroethylene

Park¹,

Nam²,

Ryu

et al. 2010

BioChip J

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Trichloroethylene (TCE), a volatile and lipophilic solvent widely used in industrial degreasing applications, is believed to be toxic in humans. To identify possible protein biomarkers of early TCE-induced hepatotoxicity, we treated rats with a single dose of TCE equivalent to 10% of LD 50 and analyzed their liver proteomes 72 h after dosing. We identified eight liver proteins that exhibited a TCE-induced change in expression level of at least 2-fold; three of these proteins were upregulated and the rest were downregulated. Because these proteins changed significantly in abundance in the absence of notable hepatotoxicity in vivo, they may be useful as one of the candidates of early predictive biomarkers of TCE exposure in rats.

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Section: Resultsmentioning

confidence: 99%

Section: Enzymatic In-gel Digestion and Mass Spectrometrymentioning

confidence: 99%

Proteomic analysis of rat liver proteins differentially induced by trichloroethylene

Park¹,

Nam²,

Ryu

et al. 2010

BioChip J

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“…Furthermore, testicular atrophy could be due to accumulation and prolonged administration of the estrogenic-like activity from Ferula harmonis causing exhaustion atrophy of the testes 9 and the ratio of free testosterone to estrogen reaches a critical point and the estrogenic gonadotropine suppressive effects predominate. 13,14 Furthermore, a report by Gunther in 1959, showed that some of the isolated compounds from Ferula genus have infertility activities. 15 In addition, cholesterol and glucose values were reduced following treatment of zallouh oil.…”

Section: Discussionmentioning

confidence: 99%

Ferula harmonis ‘zallouh’ and enhancing erectile function in rats: efficacy and toxicity study

El-Thaher¹,

Matalka

Taha

et al. 2001

Int J Impot Res

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Ferula harmonis, which is locally called`zallouh' in the Middle East, is used as an aphrodisiac as it is reputed to enhance male sexual behavior, however, there is no scienti®c veri®cation. In this study, the oil extracted from the seeds of Ferula harmonis was tested for its ef®cacy in enhancing erectile function and toxicity in male rats. The sexual activities assessed by penile erection index were dose dependent. The ED 50 (12.03 mgakg) was 880 times less than the LD 50 (10.6 gakg). However, when doses ranging from 0.05, 0.5 to 2 gakg were given daily for 28 days, acute and subacute toxicity were observed. There was a decrease in total body weight, hepatomegaly, atrophic testis, signi®cant decrease in hemoglobin and red blood cell count. In addition, there was a signi®cant decrease in cholesterol level. All the above indicate that the crude oil from the plant Ferula harmonis can enhance erectile function, however, it becomes toxic if it is used for a long period of time. Further studies are underway to isolate and identify the active ingredients and their exact mechanisms of action.

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“…This protein was expressed primarily in the mature male rat liver and excreted in the urine, and was absent from or only marginally detectable in female rats 26,27 . Initial induction and subsequent reduction of alpha-2u globulin has been recognized as one of the tumor risk factors 27 .…”

Section: Proteomic Analysis Of Differentially Expressed Proteins By Ementioning

confidence: 99%

Ethylbenzene-induced differential protein profiles in rat liver

2010

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Ethylbenzene (EB) is an important indus-trial chemical that has recently been classified as a possible human carcinogen. We investigated the effects of administration of EB equivalent to 10% and 50% of LD 50 dose on rats and analyzed their liver proteomes at 72 h after dosing. Approximately 1,100 protein spots that were detected by 2-dimensional gel electrophoresis showed reproducible abundance. 11 of these proteins showed changes in expression level of at least 1.5-fold after EB treatment. 3 of these proteins were down-regulated and the rest were up-regulated by 10% LD 50 of EB dose, whereas 4 proteins were down-regulated and the rest were up-regulated by 50% LD 50 . The differentially expressed proteins might act as a candidate of biomarker set for ED toxicity.

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Hepatic α2u-globulin mRNA levels and diethylstilbestrol-associated testicular atrophy in rats

Cited by 6 publications

References 15 publications

Proteomic analysis of rat liver proteins differentially induced by trichloroethylene

Proteomic analysis of rat liver proteins differentially induced by trichloroethylene

Ferula harmonis ‘zallouh’ and enhancing erectile function in rats: efficacy and toxicity study

Ethylbenzene-induced differential protein profiles in rat liver

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