Hepatic uptake of grape anthocyanins and the role of bilitranslocase

Passamonti, Sabina; Vanzo, Andreja; Vrhovšek, Urška; Terdoslavich, Michela; Cocolo, Alessandra; Decorti, Giuliana; Mattivi, Fulvio

doi:10.1016/j.foodres.2005.02.015

Cited by 43 publications

(35 citation statements)

References 30 publications

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“…Considering that BSP is structurally similar to Xavonoids (Passamonti et al 2002;Karawajczyk et al 2007), that rat liver BTL is an anthocyanin carrier (Passamonti et al 2002;Passamonti et al 2005b) and that the carnation petal homologue is, accordingly, competitively inhibited by cyanidine 3-glucoside (Passamonti et al 2005c), it is possible to infer that the grape homologue may be a Xavonoid carrier. Such speculation is supported by data of the competitive inhibition by quercetin (an essentially colourless Xavonoid intermediate naturally occurring in pulp berry) on the BSP transport activity and also by the expression of this carrier in speciWc compartments, as well as in diVerent developmental stages of the grape berry.…”

Section: Discussionmentioning

confidence: 99%

“…Liver BTL has a high-aYnity for dietary Xavonoids (Karawajczyk et al 2007), in particular for anthocyanins (Passamonti et al 2002). The inXux of malvidin 3-glucoside into liver cells has been shown to be strongly inhibited by BTL antibodies (Passamonti et al 2005b), not only showing the functional role of this carrier in the cellular uptake of these plant pigments, but also providing evidence that BTL antibodies can be used as molecular probes of BTL expression (Nicolin et al 2005) and function (Passamonti et al 2000) in other mammalian tissues.…”

Section: Introductionmentioning

confidence: 99%

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Evidence for a putative flavonoid translocator similar to mammalian bilitranslocase in grape berries (Vitis vinifera L.) during ripening

Braidot

Petrussa

Bertolini

et al. 2008

Planta

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During maturation, Vitis vinifera berries accumulate a large amount of several anthocyanins in the epidermal tissue, whereas their precursors and intermediates are ubiquitously synthesized within the fruit. Up to date, several mechanisms of flavonoid transport at subcellular level have been hypothesized, but it is not possible to identify a general model applicable in every plant tissue and organ. Recently, a putative anthocyanin carrier, homologue to mammalian bilitranslocase (BTL) (TC 2.A.65.1.1), was found in Dianthus caryophyllus petal microsomes. In the present paper, an immunohistochemical and immunochemical analysis, using an antibody raised against a BTL epitope, evidences the expression and function of such a transporter in V. vinifera berries (cv. Merlot). Specific localisations of the putative carrier within berry tissues together with expression changes during different developmental stages are shown. Water stress induces an increase in protein expression in both skin and pulp samples. A bromosulfalein (BSP) uptake activity, inhibitable by the BTL antibody, is detected in berry mesocarp microsomes, with K (m) = 2.39 microM BSP and V (max) = 0.29 micromol BSP min(-1) mg(-1) protein. This BSP uptake is also competitively inhibited by quercetin (K (i) = 4 microM). A putative role for this carrier is discussed in relation to the membrane transport of secondary metabolites.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evidence for a putative flavonoid translocator similar to mammalian bilitranslocase in grape berries (Vitis vinifera L.) during ripening

Braidot

Petrussa

Bertolini

et al. 2008

Planta

Self Cite

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“…Since some food sources contain substantial amounts of the anthocyanins, it needs to be determined if these , normal'doses do indeed saturate metabolic pathways and lead to absorption of the intact anthocyanin glycosides. Recently, Passamonti and colleagues [71] detected intact malvidin-3-glucoside in portal plasma after the introduction of 2 mg malvidin-3-glucoside to the stomach of a rat. The absorption of intact anthocyanin glycoside was confirmed with a level of dosing that would be unlikely to saturate in vivo metabolism.…”

Section: Anthocyanin Glycosides Affect Absorption and Are Absorbed Inmentioning

confidence: 99%

“…However, a number of studies have shown that anthocyanins are absorbed from the stomach [31,71,73], and the small intestine [31,76,79,81]. While these studies demonstrate the potential for substantial absorption, they invariably assess absorption as disappearance of anthocyanin from the test system.…”

Section: Are Anthocyanins Mainly Absorbed From the Colon As Metabolites?mentioning

confidence: 99%

The bioavailability and absorption of anthocyanins: Towards a better understanding

McGhie¹,

Walton

2007

Molecular Nutrition Food Res

505

441

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Evidence that anthocyanin compounds have beneficial effects for health are increasingly being reported in the scientific literature and these compounds are now widely recognised as potential therapeutic compounds. Berry fruit are rich sources of anthocyanins and berry fruit products or derived beverages can provide 10s to 100s of milligrams of anthocyanins in a single serve. Anthocyanins exhibit complex chemical behaviours in vitro and this will result in complex behaviour in vivo. This review attempts to summarize some aspects of anthocyanin biochemistry and discusses these in the context of what is currently known about bioavailability and absorption. Compared with other flavonoid groups, such as flavonols, relatively little is known about details and mechanisms of anthocyanin absorption and transport and much remains to be discovered.

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“…Previous in vivo studies have reported that anthocyanins are absorbed in the stomach and small intestine (Passamonti et al, 2003a;Talavera et al, 2003;Talavera et al, 2004). Felgines and colleagues administered an oral dose of a radiolabelled cyanidin 3-O-glucoside, demonstrating that the major site of absorption in mice is the intestine with a minimal accumulation of the radioactivity in tissues out of the gastrointestinal tract (Felgines et al, 2010).…”

Section: Membrane Transport Of Anthocyaninsmentioning

confidence: 99%