Hepatic oxidative stress and neurotoxicity in Pelophylax kl. esculentus frogs: Influence of long-term exposure to a cyanobacterial bloom

Gavrilović, Branka R.; Petrović, Tamara G.; Radovanović, Tijana B.; Despotović, Svetlana G.; Gavrić, Jelena P.; Krizmanić, Imre; Ćirić, Miloš; Prokić, Marko D.

doi:10.1016/j.scitotenv.2020.141569

Cited by 13 publications

(5 citation statements)

References 75 publications

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“…Amphibian species are recognized as a particularly globally threatened vertebrate taxa; we are witnessing worldwide threats faced by batrachofauna from habitat modification, environmental pollution, global warming, emerging pathogens and invasive species, all of which present a dramatic challenge for both science and society [3]. Due to their ectothermic nature, semipermeable skin and complex life cycles, amphibians are strongly dependent on specific temperature ranges, moisture, and precipitation-factors which are fundamentally affected by the process of global warming [3,4]. There is evidence that climate change, especially temperature-related change, can alter reproductive phenology, body condition and sensitivity to disease in amphibians [5].…”

Section: Introductionmentioning

confidence: 99%

Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters

Petrović

Kijanović

Tomašević

et al. 2021

Animals

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In this paper, we examined how the oxidative status (antioxidant system and oxidative damage) of Bombina variegata larvae changed during the metamorphic climax (Gosner stages: 42—beginning, 44—middle and 46—end) and compared the patterns and levels of oxidative stress parameters between individuals developing under constant water availability (control) and those developing under decreasing water availability (desiccation group). Our results revealed that larvae developing under decreasing water availability exhibited increased oxidative damage in the middle and end stages. This was followed by lower levels of glutathione in stages 44 and 46, as well as lower values of catalase, glutathione peroxidase, glutathione S-transferase and sulfhydryl groups in stage 46 (all in relation to control animals). Comparison between stages 42, 44 and 46 within treatments showed that individuals in the last stage demonstrated the highest intensities of lipid oxidative damage in both the control and desiccation groups. As for the parameters of the antioxidant system, control individuals displayed greater variety in response to changes induced by metamorphic climax than individuals exposed to desiccation treatment. The overall decrease in water availability during development led to increased oxidative stress and modifications in the pattern of AOS response to changes induced by metamorphic climax in larvae of B. variegata.

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Section: Introductionmentioning

confidence: 99%

Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters

Petrović

Kijanović

Tomašević

et al. 2021

Animals

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“…Amphibians, due to their ectothermic nature, are highly dependent on several abiotic factors (i.e., temperature) and are impacted by global warming (Petrović et al, 2021). Although amphibians are known for their high tolerance and adaptation towards environmental stress due to their intrinsic biochemical and physiological mechanisms (i.e., Bagnyukova et al, 2003;Gavrilović et al, 2021), cold-adapted populations inhabiting mountain areas are often unable to withstand the physiological stress of rising temperatures, making these species vulnerable to pathogens and climate change (i.e., El-Sayed and Kamel, 2020;Brusch et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Interdisciplinary approach to solve unusual mortalities in the European common frog (Rana temporaria) in two high-mountain ponds affected by climate change

Pastorino

Colussi

Varello

et al. 2023

Environmental Research

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“…Prolonged exposure to BGAB (equivalent to approximately 1 μg/L MCs) caused obvious oxidative damage in the liver of Pelophylax kl. esculentus ( Gavrilović et al, 2021 ). Although the gut microbiota plays an important role in amphibian digestion ( Chang et al, 2016 ), detoxification ( Zhang W. et al, 2016 ), development and immunity ( Dawood and Koshio, 2016 ), and environmental adaptation ( Bletz et al, 2017 ), the effects of MCs exposure on the expression of immune factors and composition and metabolic characteristics of the gut microbiota in amphibians have not been sufficiently studied.…”

Section: Introductionmentioning

confidence: 99%

Subchronic Toxicity of Microcystin-LR on Young Frogs (Xenopus laevis) and Their Gut Microbiota

Sun

Wang

et al. 2022

Front. Microbiol.

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Although toxic effects of microcystins (MCs) in mammals and fish have been extensively studied, the effects of MCs on the immune system and gut microbiota of amphibians have not received sufficient attention. As MCs cause general damage to the vertebrate liver and immune system and trigger an inflammatory response, and the gut microbiota is closely related to host metabolism and immunity, we speculated that MCs can cause changes in the immune system and gut microbiota of amphibians. To verify this, we examined the intestinal and liver injury of Xenopus laevis exposed to different microcystin-leucine-arginine (MC-LR) concentrations and the effects on the gut microbiota through high-throughput sequencing of 16S rDNA of the gut microbiota combined with histopathological analysis, enzyme activity determination, and qRT-PCR. Our results showed that MC-LR caused focal infiltration of inflammatory cells and increased the number of T cells and local congestion and vacuolization in X. laevis liver, but reduced the number, density, height, and regularity of villi. These liver and intestinal injuries became more obvious with an increase in MC-LR concentration. MC-LR significantly decreased the activities of malondialdehyde and alkaline phosphatase and the expression of TGF-β in the liver. Moreover, MC-LR significantly altered the gut microbiota of X. laevis. The relative abundance of Firmicutes and Bacteroidetes in high-concentration MC-LR groups was significantly reduced compared to that in low-concentration MC-LR groups, whereas Fusobacteria was significantly enriched. The metabolic gene composition of the gut microbiota in low-concentration MC-LR (≤5 μg/L) groups was significantly different from that in high-concentration MC-LR (≥20 μg/L) groups. These results deepen our understanding of the toxicity of MCs to aquatic organisms and assessment of the ecological risk of MCs in amphibians.

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Hepatic oxidative stress and neurotoxicity in Pelophylax kl. esculentus frogs: Influence of long-term exposure to a cyanobacterial bloom

Cited by 13 publications

References 75 publications

Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters

Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters

Interdisciplinary approach to solve unusual mortalities in the European common frog (Rana temporaria) in two high-mountain ponds affected by climate change

Subchronic Toxicity of Microcystin-LR on Young Frogs (Xenopus laevis) and Their Gut Microbiota

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