Hepatic iron quantification by atomic absorption spectrophotometry: Full validation of an analytical method using a fast sample preparation

Abstract

Atomic absorption spectrophotometry is considered the method of choice for hepatic iron quantification. The objective of the present study was to perform full validation assays of hepatic iron quantification by atomic absorption spectrophotometry, using a fast sample preparation procedure, following the guidelines from the International Conference on Harmonization. The following parameters were evaluated: specificity, linearity/range, precision, accuracy, limit of detection and limit of quantification. A good linear correlation was found (0.9948) in the concentration range evaluated (20- 120 ppb). The relative standard deviations were below 15% for accuracy, and below 10% for both day-to-day reproducibility and within-days precision, and the repeatability of injections was 0.65%. Limit of detection was 2 ppb, and limit of quantification was 6 ppb. Fresh bovine liver tissue was used to evaluate the procedure of collecting samples by liver biopsies. These findings indicate that hepatic iron quantification by atomic absorption spectrophotometry can be reliably performed at the established conditions, and suggest the method is suitable for further use in clinical practice. Hepatic iron quantification by AAS is validated by the experiments performed in the present study.

Post-mortem liver and bone marrow iron quantification in haemodialysis patients: A prospective cohort study

“…However, normal values for liver iron concentration measured by biochemical methods are well established and are gold standard. 15 , 18 They were used in studies comparing biochemically measured liver iron and estimated iron content by T2 MRI. 8 , 51 , 52 Also semi-quantitative scales to grade iron deposition in liver and bone marrow are based upon seminal studies that have established the "normal" and "increased" iron content.…”

“…LIC was classified as normal (≤ 36 μmol/g), mild (37-100 μmol/g), moderate (101-200 μmol/g) or severe (> 201 μmol/g) as in previous studies. 15 , 16 , 17 , 18 …”

Surface modifications affect iron oxide nanoparticles' biodistribution after multiple‐dose administration in rats

“…Atomic absorption spectroscopy (AAS) is a standard method for the determination of the iron content of biological tissues with a high level of precision. [ 24 ] AAS was used to assess the iron content throughout the liver, kidney, and spleen samples after oral administration of different nanoformulations. The organs under investigation were weighed and digested in 2 ml of nitric acid and hydrochloric acid with a ratio of 70%:36% (1:1 vol/vol), respectively, at 70°C.…”

Magnetic inductive heating of organs of mouse models treated by copolymer coated Fe 3 O 4 nanoparticles

“…We used both healthy and Sarcoma cancer transplanted mice treated by two systems of Fe 3 O 4 NPs coated by two different copolymers. For a few mouse models we performed additionally the Fe concentration analysis by using atomic adsorption spectroscopy (AAS), the method that has used earlier for experiment without MNPs [25][26][27].…”