Magnetic inductive heating of organs of mouse models treated by copolymer coated Fe ₃ O ₄ nanoparticles

Abstract

Biodistribution studies provide basic information to design and perform various applications of superparamagnetic iron oxide magnetic nanoparticles (SPIOs) in biomedicine such as drug delivery, MRI as well as hyperthermia. Recently, several quantitative measurements as well as new imaging methods have been used to characterize the SPIOs distribution in organs and in tissues of animal model. In this report we used the fabricated iron oxide nanoparticles coated with two block copolymers of polystyrene-co-polyacrylic acid (St-co-PAA) and polylactic acid-co-polyethylene glycol (PLA-PEG). The biodistributions were investigated ex-vivo for several organs of both healthy and Sarcoma transplanted Swiss mice. The SPIOs concentrations were verified mainly by magnetic inductive heating (MIH) measurement with a combination with atomic absorption spectroscopy (AAS). The results indicated the density detected highest in liver and lowest in kidney. The SPIOs concentration increased significantly up to 24 h after the injection. The observations by our two methods not only are in agreement with each other but also consistent with the tendency reported by other techniques. Discussion will also concern injection strategy for various aspects of hyperthermia applications.

Multifunctional Nanocarriers of Fe 3 O 4 @PLA-PEG/Curcumin for MRI, Magnetic Hyperthermia and Drug Delivery

“…The biodistribution of NPs depends on many factors such as chemical composition, size, shape and surface modifications [89]. In a previous report, by ex vivo MFH experiments and atomic absorption spectroscopy analysis, the authors of the current study found that when intravenously injected into mice, the amounts of Fe 3 O 4 @PLA-PEG MNPs are distributed in organs in the following order: liver > lung > spleen > tumor > kidney [90]. More important, a dose that is sufficient for hyperthermia of the tumor would overheat the liver.…”

Biocompatible Nanoclusters with High Heating Efficiency for Systemically Delivered Magnetic Hyperthermia

“…12,13 Several preclinical studies have revealed the challenges associated with elevating intratumoral temperatures above 38 °C after intravenous injection of iron oxide nanoparticles at clinically relevant doses. 14,15,16 To generate desired intratumoral temperatures, Huang et al reported that mice require intravenous injection with conventional nanoparticles at an extremely high dose of 1700 mg of iron (Fe) per kg of body weight. 12 Importantly, the recommended intravenous dosage of the FDA-approved iron oxide nanoparticle, Ferumoxytol, is 510 mg, which corresponds to 8.5 mg of iron per kilogram of body weight for a 60 kg patient.…”

The Role of Anisotropy in Distinguishing Domination of Néel or Brownian Relaxation Contribution to Magnetic Inductive Heating: Orientations for Biomedical Applications

“…For drug delivery, the MIH can be used to trigger remotely or on-site release of drug molecules being tagged to the MNPs [ 1 , 2 , 3 , 8 , 11 , 12 , 13 ]. Recently, new interesting biomedical applications were proposed including tuning the cellular gate for regulation of plasma glucose [ 14 ], biomaterials devitrification [ 15 , 16 , 17 ] and determination of MNPs accumulation in various organs [ 18 ]. In order to optimize the amount of MNPs used in the MIH, many studies focused on the conditions for maximizing heating efficiency, which is described theoretically by SLP or experimentally by specific absorption rate (SAR).…”