Hepatic Insulin Resistance in Hyperthyroid Rat Liver: Vitamin E Supplementation Highlights a Possible Role of ROS

Fasciolo, Gianluca; Napolitano, Gaetana; Aprile, Marianna; Cataldi, Simona; Costa, Valerio; Ciccodicola, Alfredo; Meo, S. Di; Venditti, Paola

doi:10.3390/antiox11071295

Cited by 14 publications

(17 citation statements)

References 74 publications

(78 reference statements)

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“…However, the results from the group fed a diet with buriti oil (BFe) suggest that the oil chemical composition was efficient to minimize the deleterious effect of the high-FeSO 4 dose on antioxidant enzyme activity. This result agrees with studies in which GPx and SOD activity evaluation in animals and humans with a β-carotene- or α-tocopherol-supplemented diet indicated the expression and activity of these enzymes, probably because of the compensatory defense of oxidative stress [ 64 , 65 , 66 , 67 ].…”

Section: Discussionsupporting

confidence: 91%

“…The high contents of β-carotene and α-tocopherol quantified in buriti oil contribute to the significant in vitro antioxidant capacity, which in turn can attenuate oxidative damage with consequent tissue recovery and biomolecules. β-carotene can quench singlet oxygen, α-tocopherol reduces mitochondrial hydrogen peroxide release rate, and both react as ROS scavengers in a nonenzymatic manner to prevent or decrease oxidative damage to biomolecules and cells [ 50 , 65 , 66 , 67 , 68 ]. Furthermore, a previous study demonstrated the synergism action among oleic acid and α-tocopherol to prevent oxidative stress [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

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Antioxidant and Lipid-Lowering Effects of Buriti Oil (Mauritia flexuosa L.) Administered to Iron-Overloaded Rats

et al. 2023

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The indiscriminate use of oral ferrous sulfate (FeSO4) doses induces significant oxidative damage to health. However, carotene-rich foods such as buriti oil can help the endogenous antioxidant defense and still maintain other body functions. This study aimed to assess the effects of buriti oil intake in iron-overloaded rats by FeSO4 administration. Buriti oil has β-carotene (787.05 mg/kg), α-tocopherol (689.02 mg/kg), and a predominance of monounsaturated fatty acids (91.30 g/100 g). Wistar rats (n = 32) were subdivided into two control groups that were fed a diet containing either soybean or buriti oil; and two groups which received a high daily oral dose of FeSO4 (60 mg/kg body weight) and fed a diet containing either soybean (SFe) or buriti oil (Bfe). The somatic and hematological parameters, serum lipids, superoxide dismutase (SOD), and glutathione peroxidase (GPx) were determined after 17 days of iron overload. Somatic parameters were similar among groups. BFe showed a decrease in low-density lipoprotein (38.43%) and hemoglobin (7.51%); an increase in monocytes (50.98%), SOD activity in serum (87.16%), and liver (645.50%) hepatic GPx (1017.82%); and maintained serum GPx compared to SFe. Buriti oil showed systemic and hepatic antioxidant protection in iron-overloaded rats, which may be related to its high carotenoid, tocopherol, and fatty acid profile.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Antioxidant and Lipid-Lowering Effects of Buriti Oil (Mauritia flexuosa L.) Administered to Iron-Overloaded Rats

et al. 2023

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“…Besides β-cell damage and/or dysfunction, oxidative stress is also one of the important triggers of insulin resistance [ 44 , 45 , 46 ]. During insulin signaling transduction, insulin stimulates autophosphorylation of the insulin receptor thereby phosphorylating and activating insulin receptor substrate, which subsequently activates phosphatidylinositol 3-kinase (PI3K) as well as the downstream phosphoinositide-dependent protein kinase-1 (PDK1) and AKT [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Selenoprotein F Knockout Caused Glucose Metabolism Disorder in Young Mice by Disrupting Redox Homeostasis

Yun

Zhou

et al. 2022

Antioxidants

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Selenoprotein F (SELENOF) might play an important role in maintaining human health since an increasing number of studies have linked SELENOF deficiency to various pathologies such as cancer and neurodegeneration. We have previously reported on glucose metabolism disorders in SELENOF knockout mice, which imply a novel biological function of SELENOF in glucose metabolism. However, the underlying mechanism and whether the effect of SELENOF on glucose metabolism is age-dependent remain unknown. In the present study, we compare the metabolic phenotype in more detail as well as the oxidative stress parameters in SELENOF knockout mice (C57BL/6J background) and naïve C57BL/6J mice of different ages (12, 16 and 21 weeks old). The results showed that SELENOF knockout caused glucose metabolism disorders only in young mice, especially in 12-week-old mice, characterized by hyperglycemia, serum insulin reduction, impaired glucose tolerance, decreased insulin sensitivity, decreased glucose catabolism, increased gluconeogenesis and impaired insulin signaling pathway. These abnormalities gradually improved with age and disappeared in knockout mice at 21 weeks old. Furthermore, before 16 weeks old, SELENOF knockout mice showed increased lipid peroxidation and decreased glutathione/glutathione disulfide ratio and glutathione peroxidase activity in the serum and liver. Furthermore, the expression of glutathione peroxidase 1 significantly reduced in the liver and pancreas. Our findings suggest that SELENOF knockout might cause glucose metabolism disorders in young mice via the disruption of redox homeostasis.

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“…Lipid and protein oxidative damages were determined in both muscle homogenates and mitochondria by determining the lipid hydroperoxides (Hps) levels [ 20 ], and protein-bound carbonyls (CO) [ 21 , 22 ].…”

Section: Methodsmentioning

confidence: 99%

“…After exposure of homogenates and mitochondria to Fe and ascorbate (Fe/Asc, 100/1000 μM, respectively) for 10 min, the susceptibility to oxidative stress was assessed by measuring the changes in hydroperoxides content [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

Muscle Oxidative Stress Plays a Role in Hyperthyroidism-Linked Insulin Resistance

et al. 2023

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While a low level of ROS plays a role in cellular regulatory processes, a high level can lead to oxidative stress and cellular dysfunction. Insulin resistance (IR) is one of the dysfunctions in which oxidative stress occurs and, until now, the factors underlying the correlation between oxidative stress and IR were unclear and incomplete. This study aims to explore this correlation in skeletal muscle, a tissue relevant to insulin-mediated glucose disposal, using the hyperthyroid rat as a model of oxidative stress. The development of IR in the liver from hyperthyroid animals has been widely reported, whereas data concerning the muscle are quite controversial. Thus, we investigated whether hyperthyroidism induces IR in skeletal muscle and the role of oxidative stress in this process. Particularly, we compared the effects of hyperthyroidism on IR both in the absence and presence of vitamin E (Vit E), acting as an antioxidant. Putative correlations between ROS production, oxidative stress markers, antioxidant capacity and changes in intracellular signalling pathways related to insulin action (AKT) and cellular stress response (EIF2α; JNK; PGC1α; BIP; and NRF1) were investigated. Moreover, we assessed the effects of hyperthyroidism and Vit E on the expression levels of genes encoding for glucose transporters (Slc2a1; Slc2a4), factors involved in lipid homeostasis and insulin signalling (Pparg; Ppara, Cd36), as well as for one of the IR-related inflammatory factors, i.e., interleukin 1b (Il1b). Our results suggest that hyperthyroidism-linked oxidative stress plays a role in IR development in muscle and that an adequate antioxidant status, obtained by vitamin E supplementation, that mitigates oxidative stress, may prevent IR development.

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Hepatic Insulin Resistance in Hyperthyroid Rat Liver: Vitamin E Supplementation Highlights a Possible Role of ROS

Cited by 14 publications

References 74 publications

Antioxidant and Lipid-Lowering Effects of Buriti Oil (Mauritia flexuosa L.) Administered to Iron-Overloaded Rats

Antioxidant and Lipid-Lowering Effects of Buriti Oil (Mauritia flexuosa L.) Administered to Iron-Overloaded Rats

Selenoprotein F Knockout Caused Glucose Metabolism Disorder in Young Mice by Disrupting Redox Homeostasis

Muscle Oxidative Stress Plays a Role in Hyperthyroidism-Linked Insulin Resistance

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