Hepatic Inflammatory Response to Exogenous LPS Challenge is Exacerbated in Broilers with Fatty Liver Disease

Mei, Wenqing; Hao, Yarong; Xie, Huilin; Ni, Yingdong; Zhao, Ruqian

doi:10.3390/ani10030514

Cited by 11 publications

(13 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From the molecular point of view, the mRNA expression of IL-1β, IL-6, TNF-α, COX-2, and iNOS was significantly increased in the liver of LPS-challenged broilers, which was consistent with enhanced mRNA expression of TLR4. Our results were in accordance with the report of Mei et al (2020) in which LPS increased the mRNA expression of NF-κB, IL-1β, IL-6, IFN-γ, and iNOS in the liver of broilers. Similar results were observed by Li et al (2015) and Liu et al (2015).…”

Section: P-valuesupporting

confidence: 93%

“…Also, this study found that supplementation with taurine could alleviate the inhibition of the ADG of the LPS-challenged broilers, indicating that taurine may exert a protective effect on the growth as evidenced by an increase in ADG of broilers under immunological stress. Moreover, many studies have shown that LPS administration causes liver injury and dysfunction in broilers (Morris et al, 2014;Qu et al, 2019;Jangra et al, 2020;Mei et al, 2020). The activities of AST and ALT in the serum are usually used clinically Means within the same row with no common superscript differ significantly (P < 0.05).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Dietary taurine supplementation attenuates lipopolysaccharide-induced inflammatory responses and oxidative stress of broiler chickens at an early age

Han

Zhang

Chen

et al. 2020

Journal of Animal Science

View full text Add to dashboard Cite

This study was conducted to investigate the effect of taurine as a prophylactic treatment on antioxidant function and inflammatory responses of broilers challenged with lipopolysaccharide (LPS). A total of 256 one-day-old male Arbor Acres broiler chicks were randomly assigned to 4 treatments with 8 replicates of 8 birds (8 birds/cage). Four treatment groups were designated as follows: 1) in the CON group, broilers fed a basal diet; 2) in the LPS group, LPS-challenged broilers fed a basal diet; 3) in the LPS + T1 group, LPS-challenged broilers fed a basal diet supplemented with 5.0 g/kg taurine; and 4) in the LPS + T2 group, LPS-challenged broilers fed a basal diet supplemented with 7.5 g/kg taurine. The LPS-challenged broilers were intraperitoneally injected with 1 mg/kg body weight (BW) of LPS at 16, 18, and 20 d of age, whereas the CON group received an injection of sterile saline. The results showed that broilers injected with LPS exhibited decreased (P < 0.05) the average daily gain (ADG) and the 21 d body weight (P < 0.05), while taurine supplementation alleviated the negative effects of LPS. Additionally, the LPS-induced increases (P < 0.05) in serum alanine transaminase and aspartate transaminase activities were reversed by taurine supplementation. The taurines could alleviate the hepatic oxidative stress, with the presence of lower content of malondialdehyde (P < 0.05), higher content of glutathione (P < 0.05), and an increased glutathione peroxidase (GSH-Px) activity (P < 0.05). The concentrations of interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) in the liver were measured by ELISA kits, and the result showed that dietary taurine supplementation prevented these cytokines increases in the liver of LPS-induced broilers. Taurine reduced the genes expression of IL-1β, TNF-α, IL-6, cyclooxygenase-2 and inducible nitric oxide synthase, whereas it boosted the expression levels of antioxidant-related genes (nuclear factor erythroid 2-related factor 2, heme oxygenase-1, glutamate-cysteine ligase catalytic subunit, and GSH-Px) in the liver of LPS-induced broilers. In conclusion, dietary taurine supplementation in broilers mitigated LPS-induced defect in ADG, oxidative stress and inflammatory responses.

show abstract

Section: P-valuesupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Dietary taurine supplementation attenuates lipopolysaccharide-induced inflammatory responses and oxidative stress of broiler chickens at an early age

Han

Zhang

Chen

et al. 2020

Journal of Animal Science

View full text Add to dashboard Cite

show abstract

“…Microbial infection is considered as a significant threat to animal production and food safety. Lipopolysaccharide (LPS), a membrane glycolipid, is a key component of gram-negative bacterial cell membrane and drives diseases (11). Animals are highly sensitive to the toxic effects of LPS.…”

Section: Introductionmentioning

confidence: 99%

Integrating Serum Metabolome and Gut Microbiome to Evaluate the Benefits of Lauric Acid on Lipopolysaccharide- Challenged Broilers

Wu¹,

Li²,

Liu³

et al. 2021

Front. Immunol.

View full text Add to dashboard Cite

Lauric acid (LA) is a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals. This study aimed to investigate the effects of LA on the intestinal barrier, immune functions, serum metabolism, and gut microbiota of broilers under lipopolysaccharide (LPS) challenge. A total of 384 one-day-old broilers were randomly divided into four groups, and fed with a basal diet, or a basal diet supplemented with 75 mg/kg antibiotic (ANT), or a basal diet supplemented with 1000 mg/kg LA. After 42 days of feeding, three groups were intraperitoneally injected with 0.5 mg/kg Escherichia coli- derived LPS (LPS, ANT+LPS and LA+LPS groups) for three consecutive days, and the control (CON) group was injected with the same volume of saline. Then, the birds were sacrificed. Results showed that LA pretreatment significantly alleviated the weight loss and intestinal mucosal injuries caused by LPS challenge. LA enhanced immune functions and inhibited inflammatory responses by upregulating the concentrations of immunoglobulins (IgA, IgM, and IgY), decreasing IL-6 and increasing IL-4 and IL-10. Metabolomics analysis revealed a significant difference of serum metabolites by LA pretreatment. Twenty-seven serum metabolic biomarkers were identified and mostly belong to lipids. LA also markedly modulated the pathway for sphingolipid metabolism, suggesting its ability to regulate lipid metabolism. Moreover,16S rRNA analysis showed that LA inhibited LPS-induced gut dysbiosis by altering cecal microbial composition (reducing Escherichia-Shigella, Barnesiella and Alistipes, and increasing Lactobacillus and Bacteroides), and modulating the production of volatile fatty acids (VFAs). Pearson’s correlation assays showed that alterations in serum metabolism and gut microbiota were strongly correlated to the immune factors; there were also strong correlations between serum metabolites and microbiota composition. The results highlight the potential of LA as a dietary supplement to combat bacterial LPS challenge in animal production and to promote food safety.

show abstract

“…Samples were rated based on the severity of steatosis, and graded 0-4, indicating none, slight, mild, moderate, and severe, respectively. Steatosis scores were analyzed independently by three veterinarians with reference to a previous publication [8]. In each case, ve tissue sections were examined and a numerical score was assigned.…”

Section: Histopathologymentioning

confidence: 99%

“…Glucocorticoid hormones (GCs) are important regulators of lipid metabolism, promoting lipogenesis when treated with chronic exposure [5].For instance, chronic excessive GCs exposure induced whole-body insulin resistance, strongly related to metabolic dysfunction including intrahepatic lipid accumulation, intramuscular fat deposition and abdominal adiposity in humans [6,7].Corticosterone (CORT) is the main active form of GC in chickens. Previous studies have shown that fatty liver disease can be induced by excessive CORT exposure in broilers [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Probiotics Supplementation Alleviate Corticosterone-induced Fatty Liver Disease Related With the Changes of Hepatic Lipogenesis and Gut Microbiota Profile in Broiler

Mei

Feng

Yao

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background:Emerging evidencesindicate a close relationship between gut microbiotaand fatty liver disease.It has been suggested that gut microbiota modulation with probiotics improved fatty liver disease in rodents and human, yet it still remains unclear in poultry.Results: Ninety six one-day-old green-legged chicken were divided into control group (CON) and probiotic group (PB), respectively. Probiotics were administrated throughdrinking water for two weeks from 1-day-old. At 28 d of age,16 broilers selectedfromCON or PB group randomly, and receivedvehicle(15% ethanol) or CORT(4.0 mg/kg)treatmentdaily viasubcutaneous injection for a week to induce fatty liver. At the end of the experiment, broilers from 4 groups,control group(CON), corticosterone group (CORT), probiotic group (PB), PB plusCORT group(CORT&PB),were slaughtered for sampling and analysis.The results showed that probiotics administration significantly prevented CORT-induced body weight loss(P<0.05), but did not alleviate the reduction of immune organs weight caused by CORT. Compared to CON,broilers in CORT group exhibited a significant increase of triglyceride (TG) levelin both plasma and liver(P< 0.01), as well as severe hepatocytic steatosis and hepatocellular ballooning, and accompanied with the up-regulation of hepatic lipogenesis genes expression. However, probiotics supplementation markedly decreased the intrahepatic lipid accumulation and steatosis histological score, which was associated withthedown-regulation of sterol regulatory element-binding protein-1 (SREBP1) and acetyl-CoA carboxylase (ACC) mRNA (P< 0.05)as well as it protein (P= 0.06) expression.Cecal microbiota composition was determined by 16S rRNA high-throughput sequencing. Results showed that CORT treatment induced distinct gut microbiota alterations with a decrease of microbial diversity, and anincrease of proteobacteriaabundance (P<0.05). On the contrary, probiotic supplementation increased the beta diversity and increased community richness and diversity index(P> 0.05), as well as the abundance of Intestinimonas(P<0.05).Conclusion: Our results indicate that CORT treatment induced serious fatty liver disease and altered the gut microbiota composition in broilers, however,probiotics supplementation from post-hatching had a beneficial effect on alleviatingfatty liver disease through regulating lipogenic genes expression and increasing gut microbiota diversity andbeneficial bacteria abundanceimbalance.

show abstract

Hepatic Inflammatory Response to Exogenous LPS Challenge is Exacerbated in Broilers with Fatty Liver Disease

Cited by 11 publications

References 37 publications

Dietary taurine supplementation attenuates lipopolysaccharide-induced inflammatory responses and oxidative stress of broiler chickens at an early age

Dietary taurine supplementation attenuates lipopolysaccharide-induced inflammatory responses and oxidative stress of broiler chickens at an early age

Integrating Serum Metabolome and Gut Microbiome to Evaluate the Benefits of Lauric Acid on Lipopolysaccharide- Challenged Broilers

Probiotics Supplementation Alleviate Corticosterone-induced Fatty Liver Disease Related With the Changes of Hepatic Lipogenesis and Gut Microbiota Profile in Broiler

Contact Info

Product

Resources

About