Hepatic fibropoiesis in dogs naturally infected with Leishmania (Leishmania) infantum treated with liposome-encapsulated meglumine antimoniate and allopurinol

Extra virgin coconut oil (Cocos nucifera L.) intake shows neurobehavioural and intestinal health effects in obesity-induced rats

“…Colon sections were performed and pre-treated for antigenic recovery using a 0.1% citrate retriever buffer solution [pH 6.0] (DAKO®, Vila Real Carpinteria, CA, code S1699) in a high temperature method and incubated in a water bath for 30 min at 97 °C. Monoclonal antibodies were used as primary antibodies as follows: (MΦ) anti-calprotectin (L1 antigen) clone MAC387 (DAKO), dilution 1 : 100; 44 anti-macrophage (MΦ) anti-CD163 clone ED2, (Santa Cruz Biotechnology), 1 : 100. 45…”

“…37 Colon sections were performed and pre-treated for antigenic recovery using a 0.1% citrate retriever buffer solution [ pH 6.0] (DAKO®, Vila Real Carpinteria, CA, code S1699) in a high temperature method and incubated in a water bath for 30 min at 97 °C. Monoclonal antibodies were used as primary antibodies as follows: (MΦ) anti-calprotectin (L1 antigen) clone MAC387 (DAKO), dilution 1 : 100; 44 anti-macrophage (MΦ) anti-CD163 clone ED2, (Santa Cruz Biotechnology), 1 : 100. 45 All slides for the morphometric analysis were incubated 18-22 h at 4 °C in a humid chamber, washed in PBS, incubated with biotinylated goat anti-mouse and anti-rabbit antibodies (LSAB2 kit; Dako), washed with PBS, and incubated with streptavidin-peroxidase complex (LSAB2 kit; Dako) for 20 min at room temperature (±26 °C).…”

Resposta imune-inflamatória a agentes infecciosos no microambiente hepático de cães e gatos: revisão

“…Este processo inflamatório granulomatoso leva à fibropoiese hepática intensa nos cães naturalmente infectados com L. (Leishmania) infantum (Melo et al, 2009). O aumento na deposição de colágeno tecidual se deve a maior expressão de TGF-β pelas células do granuloma e, então, ativação das CEH (Castro et al, 2018;Madeira et al, 2016).…”