Heparin/heparan sulfate analysis by covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry

Sanderson, Patience; Stickney, Morgan; Leach, Franklin E.; Xia, Qiangwei; Yu, Yanlei; Zhang, Fuming; Linhardt, Robert J.; Amster, I. Jonathan

doi:10.1016/j.chroma.2018.02.052

Cited by 32 publications

(37 citation statements)

References 57 publications

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“…In addition, Sanderson et al. evaluated the influence of different coatings (neutral and cationic) and additives (formic acid, diethylamine) to reduce analysis time and improve sample performance, especially for larger oligosaccharides . Utilizing a cationic coating, it was possible to differentiate between stereoisomeric tetrasaccharides, showing the potential of these methods.…”

Section: Analytical Applicationsmentioning

confidence: 99%

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

et al. 2018

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Capillary electrophoresis (CE) offers fast and high‐resolution separation of charged analytes from small injection volumes. Coupled to mass spectrometry (MS), it represents a powerful analytical technique providing (exact) mass information and enables molecular characterization based on fragmentation. Although hyphenation of CE and MS is not straightforward, much emphasis has been placed on enabling efficient ionization and user‐friendly coupling. Though several interfaces are now commercially available, research on more efficient and robust interfacing with nano‐electrospray ionization (ESI), matrix‐assisted laser desorption/ionization (MALDI) and inductively coupled plasma mass spectrometry (ICP) continues with considerable results. At the same time, CE‐MS has been used in many fields, predominantly for the analysis of proteins, peptides and metabolites. This review belongs to a series of regularly published articles, summarizing 248 articles covering the time between June 2016 and May 2018. Latest developments on hyphenation of CE with MS as well as instrumental developments such as two‐dimensional separation systems with MS detection are mentioned. Furthermore, applications of various CE‐modes including capillary zone electrophoresis (CZE), nonaqueous capillary electrophoresis (NACE), capillary gel electrophoresis (CGE) and capillary isoelectric focusing (CIEF) coupled to MS in biological, pharmaceutical and environmental research are summarized.

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Section: Analytical Applicationsmentioning

confidence: 99%

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

et al. 2018

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“…If coated emitters are required, then covalently bound coatings are best because they are stable and effective. 3…”

Section: Discussionmentioning

confidence: 99%

“…17 For all experiments, the separation capillary was modified with AHS as described in our previous paper. 3…”

Section: Emitter Surface Modificationmentioning

confidence: 99%

“…1,2 Recently our laboratory demonstrated the effectiveness of CZE-MS for analyzing glycosaminoglycans (GAGs), a family of sulfated carbohydrates that are difficult to analyze by LC-MS/MS. 3 A commercial sheath flow CZE-MS interface provided the means to implement nano-electrospray with a low dilution factor and minimal sample consumption, while providing ample resolution of closely related isomers.…”

Section: Introductionmentioning

confidence: 99%

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Investigation of electrospray for a capillary electrophoresis–mass spectrometry interface in reverse polarity and negative ion mode

Stickney

Xia²,

Amster

2019

Eur J Mass Spectrom (Chichester)

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Capillary zone electrophoresis (CZE) paired with mass spectrometry (MS) is a powerful analytical technique for examining mixtures of ionic analytes such as glycosaminoglycans. This study examines the mechanics of the electrospray process for a sheath flow CZE-MS interface under reverse polarity negative ionization conditions. Liquid flow in a sheath flow nanoelectrospray CZE-MS interface is driven by two mechanisms, electroosmotic flow and electrospray nebulization. The contribution of these two processes to the overall flow of solution to the electrospray tip is influenced by the surface coatings of the sheath flow emitter tip and by the solvent composition. We have investigated the application of this interface to the reverse polarity separation of glycosaminoglycans and find that the role of electroosmotic flow is far less than has been reported previously, and the electrospray process itself is the largest contributor to the flow of the sheath liquid.

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“…to show the presence of isomers (71,134). An example of this is shown in Figure 10; four tetrasaccharide epimers were separated based on difference of the C-5 stereochemistry on the uronic acid residues.…”

Section: Negative Electron Transfer Dissociation (Netd) Is Another Usmentioning

confidence: 99%

Developments in Mass Spectrometry for Glycosaminoglycan Analysis: A Review

Pepi

Sanderson

Stickney

et al. 2021

Molecular & Cellular Proteomics

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This review covers recent developments in glycosaminoglycan (GAG) analysis via mass spectrometry (MS). GAGs participate in a variety of biological functions, including cellular communication, wound healing, and anticoagulation, and are important targets for structural characterization. GAGs exhibit a diverse range of structural features due to the variety of O- and N-sulfation modifications and uronic acid C-5 epimerization that can occur, making their analysis a challenging target. Mass spectrometry approaches to the structure assignment of GAGs have been widely investigated, and new methodologies remain the subject of development. Advances in sample preparation, tandem MS techniques (MS/MS), on-line separations and automated analysis software have advanced the field of GAG analysis. These recent developments have led to remarkable improvements in the precision and time efficiency for the structural characterization of GAGs.

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Heparin/heparan sulfate analysis by covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry

Cited by 32 publications

References 57 publications

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

Investigation of electrospray for a capillary electrophoresis–mass spectrometry interface in reverse polarity and negative ion mode

Developments in Mass Spectrometry for Glycosaminoglycan Analysis: A Review

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