Heparan Sulfate Proteoglycans and Viral Attachment: True Receptors or Adaptation Bias?

Cagno, Valeria; Tseligka, Eirini D.; Jones, Samuel T.; Tapparel, Caroline

doi:10.3390/v11070596

Cited by 302 publications

(415 citation statements)

References 197 publications

Supporting

Mentioning

399

Contrasting

Unclassified

Order By: Relevance

“…Based on a previous work addressing the mechanism involved in the activity of bLf against a SARS pseudovirus (Lang et al, 2011), it is likely that inhibition of SARS-CoV-2 infection occurs at the entry level by blocking heparan sulfate proteoglycans (HSPGs), which are commonly exploited by viruses for cell attachment (Cagno et al, 2019). Indeed, this seems to be a general mechanism underlying the inhibitory effect of bLf on several unrelated viruses, including respiratory viruses (Superti et al, 2019) and even endemic (Carvalho et al, 2014) or epidemic (Carvalho et al, 2017) arboviruses.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Inhibition of SARS-CoV-2 Infection by Bovine Lactoferrin

Carvalho

Matos

Caetano

et al. 2020

Preprint

View full text Add to dashboard Cite

Since its emergence in late 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been posing a serious threat to public health worldwide as the causative agent of coronavirus disease 2019 . Now distributed in a pandemic pattern, this disease still lacks an effective drug treatment with low toxicity, leading pharmaceutical companies and research labs to work against time to find a candidate molecule to efficiently treat the affected patients. Due to the well-known broad-spectrum antimicrobial activity of the lactoferrin protein, we sought to verify whether its bovine form (bLf) would also be effective in vitro against SARS-CoV-2. Using an antiviral assay based on quantitative reverse transcription-polymerase chain reaction (qRT-PCR), we found that bLf reduced progeny virus yield by up to ~84,6% in African green monkey kidney epithelial cells (Vero E6) and ~68,6% in adenocarcinomic human alveolar basal epithelial cells (A549) at 1 mg/mL, a concentration previously shown to have low cytotoxicity. Therefore, our preliminary data suggest that bLf has the potential to constitute a biochemical approach to fight the new coronavirus pandemic. _______________________________________________________________________________________

show abstract

Section: Discussionmentioning

confidence: 99%

In Vitro Inhibition of SARS-CoV-2 Infection by Bovine Lactoferrin

Carvalho

Matos

Caetano

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Glycosylation refers to the addition of a carbohydrate or glycan to a noncarbohydrate structure that can occur in almost all surface components including proteins and lipids. Glycosylation has many different forms such as N-and O-glycosylation, glycosylphosphatidylinositol glycosylation and lipid glycosylation, with the amount, distribution and type varying across species (Cagno et al 2019). These characteristics substantially restrict glycosylation in being considered as targets for surface modulation.…”

Section: Opportunities and Challengesmentioning

confidence: 99%

Host receptors: the key to establishing cells with broad viral tropism for vaccine production

Dai

Zhang

Ostrikov

et al. 2020

Critical Reviews in Microbiology

View full text Add to dashboard Cite

Cell culture-based vaccine technology is a flexible and convenient approach for vaccine production that requires adaptation of the vaccine strains to the new cells. Driven by the motivation to develop a broadly permissive cell line for infection with a wide range of viruses, we identified a set of the most relevant host receptors involved in viral attachment and entry. This identification was done through a review of different viral entry pathways and host cell lines, and in the context of the Baltimore classification of viruses. In addition, we indicated the potential technical problems and proposed some solutions regarding how to modify the host cell genome in order to meet industrial requirements for mass production of antiviral vaccines. Our work contributes to a finer understanding of the importance of breaking the host-virus recognition specificities for the possibility of creating a cell line feasible for the production of vaccines against a broad spectrum of viruses. ARTICLE HISTORY

show abstract

“…19 In fact many viruses, not just coronaviruses, utilise heparan sulfate on cell surfaces for initial binding. 29 Feline infectious peritonitis virus uses CD13 (aminopeptidase N) or CD209 to bind to its host cells. 15 Bovine coronavirus binds to 9-O-acetylated sialic acid on its host cells.…”

Section: Coronavirusesmentioning

confidence: 99%