Hemostatic implant based on cellulose derivates

Abstract

Aim of research: to study the comparative effectiveness of new polycompositive of hemostatic implant on in vivo model of liver parenchymal bleeding.Material and methods. Experimental studies were performed using the developed polycompositive hemostatic implant (CPM) from cellulose derivatives. Main components of implant include: natrium carboxymethylcellulose, oxidized cellulose, nanocellulose and bonded calcium ions. In vivo experiments were conducted in both sexes white 24 utbred rats weighing 196.5 ± 2.8 grams. The animals were divided into two groups: the comparative group (n = 24) with an application of polycomposite hemostatic implant and main group (n = 24) with medical gauze. The hemostatic materials were used in equal weight 30 mg. All surgical procedures on animals were performed under general inhalation anesthesia in model of liver parenchymal bleeding.Results. Thus, hemostasis was reached within 34.0 ± 2.5 seconds in the main group with CPM, in the comparative group with medical gauze — 142.2±7.7 seconds. Repeated bleeding was observed in 11 (45.8%) cases of the comparative group and in the main group — 2 (8.3%) cases. Biodegradation of the hemostatic implant and regenerative processes in the liver parenchyma were registered on the 14 day, especially in the injured area that indicates the restoration of liver tissue. In the comparative group on the 30th day, the preservation of medical gauze structure was revealed without degradation signs.Findings. According to our research data, a polycompositive hemostatic implant adheres tightly to the liver tissue, stops bleeding without its recurrence. In histological studies conducted in the dynamics of wound healing of the liver it was found that the implant does not cause a severe inflammatory reaction and biodegradation occurs after 14 days.