Hemopoietic Effect of Extracts from Four Parts of Deer Antler on Phenylhydrazine-Induced Hemolytic Anemia in Female Rats

이미라,; 김현호,; 조현호,; 강효진,; 고리주안,; 이선영,; 이청하,; 김승미,; 양선아,; 모은경,; 성창근,

doi:10.3746/jkfn.2009.38.12.1718

Cited by 26 publications

(25 citation statements)

References 17 publications

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“…Negative effect was observed mainly with the S. longepedunculata extract; the mechanism is not understood. Inflammation can also affect number of red blood cells and haemoglobin meaning inflammatory anemia (Berger, 2007;Lee et al, 2014). That effect was not been observed in our study probably due to the impact delay on those parameters.…”

Section: Discussioncontrasting

confidence: 52%

Antibacterial and Anti-Inflammatory Activities of Crude Extracts of Three Togolese Medicinal Plants Against Esbl Klebsiella Pneumoniae Strains

Toudji

Thiombiano

Karou³

et al. 2017

Afr J Tradit Complement Altern Med

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Background: Pithecellobium dulce (Roxb.) Benth., Securidaca longepedunculata Fresn and Cryptolepis sanguinolenta (Lindl.) Schlt are three plants widely used in the Togolese traditional medicine to treat microbial infections. Some studies reported their antibacterial activity alone but until know there no data concerning their possible interaction with conventional antibiotics. The main objective of this study was to investigate the antibacterial activity of the association of the crude extracts of the three plants with some conventional antibiotics. We further evaluate the antioxidant and the anti-inflammatory activities of the extracts on rat's model. Materials and methods:The antimicrobial activity was evaluated by the broth microdilution assay and the Fractional inhibitory concentrations (FIC) determined by the checkerboard method. The anti-inflammatory activity was evaluated using the Carrageenan-induced rat paw edema model. The antioxidant activities and the phenol contents were determined by spectrophotometry. Results: The MICs of hydroethanolic extract of plants ranged from 3.125 to 100 mg/mL on Klebsiella pneumoniae strains. Synergistic action was observed only with the combination of Imipenem/P. dulce, imipenem/C. sanguinolenta, amikacin/P. dulce and amikacin/C. sanguinolenta against the ESBL negative Klebsiella pneumoniae strain. Of the 21 associations, 15 were antagonistic on the ESBL-producing strains. The indifference effect was observed with the combination of the extract of Securidaca longepedunculata and the following antibiotics imipenem, amikacin, tetracyclin, ciprofloxacin, Cefotaxim; and Sulfametoxazol+Trimethoprim. The in vitro anti-inflammatory with Lipoxygenase inhibition activity was best with C. sanguinolenta extract while the in vivo paw edema model revealed that S. longepedunculata was the highest reducer of paw edema. In addition white blood cells count and biochemical parameters such as total proteins and immunoglobulins were significantly affected by the administration of plant extracts. Conclusion: This study revealed that the three plants although they may inhibit the bacterial growth by themselves, but there is also a possible synergistic action with the commercial antibiotics. Further investigations are needed to identify the active compounds and their mechanism of action.

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Section: Discussioncontrasting

confidence: 52%

Antibacterial and Anti-Inflammatory Activities of Crude Extracts of Three Togolese Medicinal Plants Against Esbl Klebsiella Pneumoniae Strains

Toudji

Thiombiano

Karou³

et al. 2017

Afr J Tradit Complement Altern Med

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“…Previous studies indicated hepcidin expression was repressed in phenylhydrazine (PHZ)-induced hemolytic anemia [18][19][20][21][22]. Nonetheless, the mechanisms for the pathogenic role of erythroid regulator in hepcidin suppression upon PHZ-induced hemolysis are elusive, and specifically, no insight has been gained into physiological hepcidin suppression of hemolytic anemia in a long term course.…”

Section: Introductionmentioning

confidence: 99%

EPO-dependent induction of erythroferrone drives hepcidin suppression and systematic iron absorption under phenylhydrazine-induced hemolytic anemia

Jiang

Gao

Chen

et al. 2016

Blood Cells, Molecules, and Diseases

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a b s t r a c t a r t i c l e i n f oHemolytic anemia is a common form of anemia due to hemolysis, resulting in disordered iron homeostasis. In this study, a dose of 40 mg/kg phenylhydrazine (PHZ) was injected into mice to successfully establish a pronounced anemia animal model, which resulted in stress erythropoiesis and iron absorption. We found that serum erythropoietin (EPO) concentration was dramatically elevated by nearly 5000-fold for the first 2 days, and then drop to the basal level on day 6 after PHZ injection. Mirrored with serum EPO concentration, the mRNA expression of erythroferrone (ERFE) was rapidly increased in the bone marrow and spleen 3 days after injection of PHZ, and then gradually decreased but was still higher than baseline on day 6. In addition, we also found that the hepcidin mRNA levels were gradually reduced almost up to 8-fold on day 5, and then was ameliorated compared to the untreated control. Mechanistic investigation manifested that the increase of serum EPO essentially determined the induction of ERFE expression particular at the first 3 days after PHZ treatment. Lentiviral mediated ERFE knockdown significantly restrained hepcidin suppression under PHZ treatment. Thus, our data unearthed EPOdependent ERFE expression acts as an erythropoiesis-driven regulator of iron metabolism under PHZ-induced hemolytic anemia.

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“…However, there was a significant (p<0.05) decrease in the body mass in groups III and IV, respectively at the day D21. The anemia-induced group injected with PHZ showed a significant decrease in mass compared to that in the normal group (P<0.05) [38]. Mass loss in the PHZ-treated group suggested that nutrition deteriorated due to oxidation losses in tissues from oxygen free radicals due to the automatic oxidation of PHZ, a strong oxidant [39].…”

Section: Iron Contentmentioning

confidence: 99%

The anti-anemic effect of dried beet green in phenylhydrazine treated rats

Elaby

Ali

2018

Archives of Pharmaceutical Sciences Ain Shams University

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Phenylhydrazine (PHZ) and its derivatives were used firstly as antipyretics but the toxic action on red blood cells made their use dangerous. The aim of this study was to estimate the contents of iron and vitamins in different parts of beetroot as root and beet green (leave and stalk together) as well as their ethanol extracts. Then, the biochemical evaluation for the highest iron and vitamins contents as an anti-anemic effect against anemic rats caused by phenylhydrazine were studied. Anemia induction caused by intraperitoneal injection of phenylhydrazine at 40 mg/kg for 2 days. Rats were randomly divided into four groups (n=10/group). Group, I fed a standard diet (normal control). Group II fed standard diet contained 5% dried beet green for normal rats. Group III fed a standard diet for anemic rats. Group IV fed a standard diet containing 5% dried beet green for anemic rats. It was assayed the complete blood count (CBC): red blood cell (RBC) count, hemoglobin concentration (Hb) and haematocrit percentage (HCT) as indices measured of anaemia as well as white blood cell (WBC) count at the third day (D3), twenty-one day (D21) and forty-two day (D42). Also, the activity of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), the levels of serum urea, uric acid, creatinine and malondialdehyde (MDA), plasma hydrogen peroxide (H2O2) and total antioxidants capacity (TAC) contents were analyzed according to manufacturer's instructions. The dried beet green is a rich source of iron and vitamins contents. After feeding on standard diet containing 5% dried beet green for anemic rats, showed that levels of RBC, HB and HCT were increased and the WBC count was improved compared to anemic control group. The activity of ALT, AST, and ALP, the levels of serum urea, creatinine and MDA and plasma H2O2 content were significantly ameliorated while the TAC was significantly increased compared to the anemic control group. In addition, the results of the histological examination coincided with the results of blood analysis. We conclude that nutrition of 5% dried beet green for forty-two days is safe, non-toxic and reduces anemia.

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Hemopoietic Effect of Extracts from Four Parts of Deer Antler on Phenylhydrazine-Induced Hemolytic Anemia in Female Rats

Cited by 26 publications

References 17 publications

Antibacterial and Anti-Inflammatory Activities of Crude Extracts of Three Togolese Medicinal Plants Against Esbl Klebsiella Pneumoniae Strains

Antibacterial and Anti-Inflammatory Activities of Crude Extracts of Three Togolese Medicinal Plants Against Esbl Klebsiella Pneumoniae Strains

EPO-dependent induction of erythroferrone drives hepcidin suppression and systematic iron absorption under phenylhydrazine-induced hemolytic anemia

The anti-anemic effect of dried beet green in phenylhydrazine treated rats

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