Hemolytic Interactions of Dermatophilus congolensis

Abstract

The strains of Dermatophilus congolensis grew on blood agar with washed sheep erythrocytes with marked total hemolysis. In testing for hemolytic interactions they gave a significant synergistic effect of a characteristic shape with Rhodococcus equi and Streptococcus agalactiae, whereas with Staphylococcus aureus producing beta hemolysin and with Staphylococcus aureus producing delta hemolysin a simultaneous synergistic as well as antagonistic effect were observed. First of all a conspicuous inhibition of in the beta hemolysin zone began and then the hemolytic effect of D. congolensis was enhanced. A similar double reaction was also observed with Listeria ivanovii. With delta hemolysin there was an inhibition of the hemolytic effect of D. congolensis and at the same time a synergistic effect could be observed. Also D. congolensis gave a weak synergistic effect with Micrococcus lylae and Licteria monocytogenes, and a further weak antagonistic effect with alpha hemolysin of Staphylococcus aureus, Staphylococcus hyicus, Staphylococcus cbromogenes and Micrococcus luteus.

Antigenicity of Dermatophilus Congolensis Hemolysin

“…The found differences of DCH concentrations necessary for direct and synergistic hemolysis are in accordance with the earlier description (5). The zone of direct hemolysis was, however, always incomparably smaller than the zone of synergistic hemolytic effect.…”

“…The same bacterial strains were used as described previously (5). Five strains of Dermatophilus congolensis, namely one strain isolated by us (3), two strains provided from NCTC, 5175 and 7915, and two strains provided from the Norwegian College of Veterinary Medicine, Oslo, N-A and N-L.…”

“…In our previous study we described hemolytic interactions of native exosubstance produced by growing strains of D . congolensis, particularly its conspicuous synergism with delta hemolysin of Staphylococcus aureus, CAMP factor of Streptococcus agalactiae and equi factor of Rhodococcus equi, the same as its marked antagonism with beta hemolysin of Staphylococcus aureus (5).…”

Evaluation of randomly amplified polymorphic DNA and pulsed field gel electrophoresis techniques for molecular typing ofDermatophilus congolensis

“…In thios study we tested 24 restriction enzymes with 6-8 base-pair recognition sites. Only Sse8387I yielded wellresolved patterns composed of a limited number (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) of fragments, as recommended [72]. Other enzymes tested yielded excessive numbers of small fragments, that were impossible to resolve by PFGE.…”

Dermatophilus congolensis infection in sheep and goats in Delta region of Tamil Nadu

“…Even though the pathogenesis of dermatophilosis is not well understood, it is reported that DC is hemolytic [ 17 ] and produces phospholipases [ 18 ], proteolytic enzymes [ 19 , 20 ], alkaline ceramidase [ 6 ], and serine protease [ 7 ]. Many of these virulence factors were not characterized widely in DC isolates of the different geographical area, but these studies reported a high degree of phenotypic interstrain variation between DC strains.…”