Antigenicity of Dermatophilus Congolensis Hemolysin

Skalka, Boris; Pospíśil, L

doi:10.1111/j.1439-0450.1993.tb00130.x

Cited by 3 publications

(2 citation statements)

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“…With the aim to make macroscopic diagnosis of D. congolensis on culture media more accurate our previous reports have described haemolysin interactions of this species with exosubstances of other bacterial species, particularly with CAMP factor of Streptococcus agalactiae, equi factor of Rhodococcus equi and staphylococcal beta and delta haemolysins ( S k a I k a and Po s pis i I 1992) as well as the possibility of serum diagnosis on the basis of the demonstration of antigenic identity of haemolysin produced by various D. congolensis strains (Skalka and PospiSil 1993a).…”

Section: Discussionmentioning

confidence: 99%

A standard method for demonstration of the life cycle of Dermatophilus congolensis

Skalka¹,

Pospíśil²

1994

Acta Vet. Brno

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Skalka B. and L. PospiSil: A standardmethodfordemonstration of the life cycle of Derma tophi Ius congolensis. Acta vet. Bmo, 62,1994: 3-7. Detennination of the life cycle of Dermatophilus congolensis is a prerequisite for its diagnosis. Attempts to demonstrate the life cycle of D. congolensis in preparations are successful only now and then even when nutrient-rich media for its cultivation are used. Enrichment of the media with sterile rabbit blood serum stimulates typical septation of the hyphae and intratubular development of the spores, which is the unique characteristic of this species. Preparations from the media enriched with blood serum regularly contain all stages of the life cycle of D. congolensis and thus make its accurate diagnosis possible.Dermatophilus congolensis, life cycle, enrichment of media with sterile serum. Van Saceghem 1915, 357, emend. mut. char. Gordon 1964, the causative agent of dermatophilosis of animals and man, is characterized by its unique life cycle, at the beginning and at the end of which are motile zoospores of 0.5 JlID in diameter. Upon germination they lose flagella and develop into tubular forms, generally up to 5 JlID wide and extremely long, branching laterally at right angles and slightly conical in form. The substrate mycelium developing on solid medium gives rise to a colony. This process also takes place in liquid medium, being manifested by a membrane and by the development of flocculi or small granules. The internal transverse and longitudinal branching of tubular hyphae creates septa in which new spores develop. After being released from the septa the zoospores are motile for a certain period of time and their gennination marks the beginningofanewlifecycle (Roberts 1961;Gordon and Edwards 1963; Gyles 1986;Gordon 1989). D. congolensis is intensively Gram-positive. Dermatophilus congolensisAttemps to detect the life cycle of D. congolensis in fixed stained preparations often fail to yield positive results because of different multiplication rates of the strains and because of the culture medium employed (P 0 s P i § i 1 et al. 1992). To detennine the conditions pennitting regular demonstration of the life cycle of this species in fixed stained preparations was the objective of the present study. Materials and Methods Prerarations for microscopic examinationAll five D. congolensis strains growing in the afore-mentioned media were examined by microscope in fixed preparations stained according to Gram. The materials were collected after 14 h, 18 h, 24 h, 36 h and 48 h

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Section: Discussionmentioning

confidence: 99%

A standard method for demonstration of the life cycle of Dermatophilus congolensis

Skalka¹,

Pospíśil²

1994

Acta Vet. Brno

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“…Such necrosis was presumably due to the keratolytic enzymes produced by D congolensis. 15 In addition, a haemolytic substance 16 produced by D congolensis may exacerbate the condition, although its role in the pathogenesis of dermatophilosis is uncertain. In crocodiles in the present study, as in cattle, it was presumed that Dermatophilus sp had invaded uninjured epidermis.…”

Section: Discussionmentioning

confidence: 99%