Hemolysis Near an Ultrasonically Pulsating Gas Bubble

Rooney, James A.

doi:10.1126/science.169.3948.869

Cited by 226 publications

(121 citation statements)

References 7 publications

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“…Reports of thresholds for producing cell membrane damage or increased permeability are abundant [12,27,28,36,39]. Interestingly, some of these thresholds are in the region of stable cavitation, indicating that collapse cavitation is not required for cell membrane damage [39].…”

Section: Discussionmentioning

confidence: 99%

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The role of cavitation in acoustically activated drug delivery

Husseini

Rosa

Richardson

et al. 2005

Journal of Controlled Release

152

139

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Pluronic P105 micelles are potential candidates as chemotherapy drug delivery vehicles using ultrasonic stimulation as a release trigger. Acoustic power has been previously shown to release two anthracycline agents from these polymeric carriers. In this study, an ultrasonic exposure chamber with fluorescence detection was used to examine the mechanism of doxorubicin release from P105 micelles. Acoustic spectra were collected and analyzed, at the same spatial position as fluorescence data, to probe the role of cavitation in drug release. Our study showed a strong correlation between percent drug release and subharmonic acoustic emissions, and we attribute the drug release to collapse cavitation that perturbs the structure of the micelle and releases drug.

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Section: Discussionmentioning

confidence: 99%

“…Interestingly, some of these thresholds are in the region of stable cavitation, indicating that collapse cavitation is not required for cell membrane damage [39]. There is a reported threshold for DNA delivery to rabbit endothelial cells of about 2,000 W/ cm 2 at 0.85 MHz (pulse average intensity) [28].…”

Section: Discussionmentioning

confidence: 99%

The role of cavitation in acoustically activated drug delivery

Husseini

Rosa

Richardson

et al. 2005

Journal of Controlled Release

152

139

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“…Such oscillation creates a circulating fluid flow (called microstreaming) around the bubble [7][8][9] with velocities and shear rates proportional to the amplitude of the oscillation. At high amplitudes the associated shear forces are capable of shearing open red cells [10] and synthetic vesicles such as liposomes [7].…”

Section: Cavitationmentioning

confidence: 99%

Ultrasonic drug delivery – a general review

Pitt

Husseini

Staples

2004

Expert Opinion on Drug Delivery

543

428

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Ultrasound (US) has an ever-increasing role in the delivery of therapeutic agents including genetic material, proteins, and chemotherapeutic agents. Cavitating gas bodies such as microbubbles are the mediators through which the energy of relatively non-interactive pressure waves is concentrated to produce forces that permeabilize cell membranes and disrupt the vesicles that carry drugs. Thus the presence of microbubbles enormously enhances delivery of genetic material, proteins and smaller chemical agents. Delivery of genetic material is greatly enhanced by ultrasound in the presence of microbubbles. Attaching the DNA directly to the microbubbles or to gas-containing liposomes enhances gene uptake even further. US-enhanced gene delivery has been studied in various tissues including cardiac, vascular, skeletal muscle, tumor and even fetal tissue. US-enhanced delivery of proteins has found most application in transdermal delivery of insulin. Cavitation events reversibly disrupt the structure of the stratus corneum to allow transport of these large molecules. Other hormones and small proteins could also be delivered transdermally. Small chemotherapeutic molecules are delivered in research settings from micelles and liposomes exposed to ultrasound. Cavitation appears to play two roles: it disrupts the structure of the carrier vesicle and releases the drug; it also makes the cell membranes and capillaries more permeable to drugs. There remains a need to better understand the physics of cavitation of microbubbles and the impact that such cavitation has upon cells and drug-carrying vesicles.

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“…Drug delivery and gene transfection had been accomplished in this way, but the precise interaction between oscillating or collapsing bubbles and cells had not been identified. Micrometer-scale acoustic streaming has been proposed as a possible mechanism for membrane permeation: high shear rates submit the cells to strong stresses (21).…”

mentioning

confidence: 99%

A bubble-driven microfluidic transport element for bioengineering

Marmottant

Hilgenfeldt

2004

Proc. Natl. Acad. Sci. U.S.A.

168

158

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Microfluidics typically uses channels to transport small objects by actuation forces such as an applied pressure difference or thermocapillarity. We propose that acoustic streaming is an alternative means of directional transport at small scales. Microbubbles on a substrate establish well controlled fluid motion on very small scales; combinations (''doublets'') of bubbles and microparticles break the symmetry of the motion and constitute flow transport elements. We demonstrate the principle of doublet streaming and describe the ensuing transport. Devices based on doublet flow elements work without microchannels and are thus potentially cheap and highly parallelizable.

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Hemolysis Near an Ultrasonically Pulsating Gas Bubble

Cited by 226 publications

References 7 publications

The role of cavitation in acoustically activated drug delivery

The role of cavitation in acoustically activated drug delivery

Ultrasonic drug delivery – a general review

A bubble-driven microfluidic transport element for bioengineering

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