Hemolysis and cytotoxicity mechanisms of biodegradable magnesium and its alloys

Zhen, Zhen; Liu, Xiaoli; Huang, Tao; Xi, Tao; Zheng, Yufeng

doi:10.1016/j.msec.2014.08.038

Cited by 141 publications

(93 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The direct culture assays refer to the cell viability of a cell culture directly on the surface of the alloys, while the indirect culture assays refer to the investigation of the effect of degradation products from the alloy by a culture medium on cell growth [51]. Both methods are affected by various experimental factors, such as the use of different alloys, cell lines, and medium types [12,16,[51][52][53][54][55][56][57][58][59][60][61].…”

Section: In Vitro Cytocompatibility Evaluations Of Mg-based Materialsmentioning

confidence: 99%

“…A surface area to extraction medium ratio of 1.25 cm 2 mL is used in many studies [12,53,68,88,89], based on recommendations of ISO 10993-12 for the standard surface area and extract liquid volumes (Table 1) [90]. According to the ISO standard, the extraction medium should be able to extract the maximum amount of extractable substance(s) within physiologically relevant surface: volume ratios for biological testing [90].…”

Section: Indirect Culture Assaysmentioning

confidence: 99%

“…The degradation rates may be reduced in vitro by modifying the extracted environment such as more supplement of serum into cell culture medium, as mentioned above. Alternatively, it has been recommended to dilute the extracts to different concentrations [53,63,64], which helps for matching the in vivo findings more closely.…”

Section: Indirect Culture Assaysmentioning

confidence: 99%

See 2 more Smart Citations

In vitro and in vivo cytocompatibility evaluation of biodegradable magnesium-based stents: a review

et al. 2018

View full text Add to dashboard Cite

Biodegradable magnesium (Mg)-based vascular stents have been designed as temporary scaffolds to treat angiostenotic lesions for the maintenance of normal blood flow. Numerous studies have presented in vitro and in vivo tests for the evaluation of the safety and feasibility of Mg-based vascular stents and the related materials. Therein the cytocompatibility is a basic and important parameter in the evaluation system. In this review, we summarize the applications and limitations of in vitro evaluation methods including basic characterization methods and direct and indirect cytotoxicity tests. We discuss the influencing factors on cytotoxicity, such as surface roughness, preconditioning of sample surface, cell type for the biocompatibility evaluation in direct contact as well as conditions for the formation of extracts/ degradation products for indirect assays. Besides, we highlight the recent in vivo animal tests and clinical trials about Mgbased stents along with some associated results. The aim of this review is to provide a meaningful reference in the further developments and related evaluation methods of Mg-based stents.

show abstract

Section: In Vitro Cytocompatibility Evaluations Of Mg-based Materialsmentioning

confidence: 99%

Section: Indirect Culture Assaysmentioning

confidence: 99%

See 1 more Smart Citation

In vitro and in vivo cytocompatibility evaluation of biodegradable magnesium-based stents: a review

et al. 2018

View full text Add to dashboard Cite

show abstract

“…It was considered that the high hemolysis rate is closely related to the local alkalization induced by the fast corrosion of the pristine magnesium alloy. A recent piece of literature reported that the high pH value caused by the fast degradation of magnesium alloy can lead to a high hemolysis ratio [28]. On the other side, a high pH value can increase the binding ability of hemoglobin to the membrane and subsequently increase the hemolysis rate, and the red blood cells can fuse with Ca 2+ of the medium, thus resulting in a rupture of erythrocytes, finally leading to serious hemolysis [29].…”

Section: Anticoagulationmentioning

confidence: 99%

Improving Corrosion Resistance and Biocompatibility of Magnesium Alloy by Sodium Hydroxide and Hydrofluoric Acid Treatments

et al. 2016

View full text Add to dashboard Cite

Abstract:Owing to excellent mechanical property and biodegradation, magnesium-based alloys have been widely investigated for temporary implants such as cardiovascular stent and bone graft; however, the fast biodegradation in physiological environment and the limited surface biocompatibility hinder their clinical applications. In the present study, magnesium alloy was treated by sodium hydroxide (NaOH) and hydrogen fluoride (HF) solutions, respectively, to produce the chemical conversion layers with the aim of improving the corrosion resistance and biocompatibility. The results of attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS) indicated that the chemical conversion layers of magnesium hydroxide or magnesium fluoride were obtained successfully. Sodium hydroxide treatment can significantly enhance the surface hydrophilicity while hydrogen fluoride treatment improved the surface hydrophobicity. Both the chemical conversion layers can obviously improve the corrosion resistance of the pristine magnesium alloy. Due to the hydrophobicity of magnesium fluoride, HF-treated magnesium alloy showed the relative better corrosion resistance than that of NaOH-treated substrate. According to the results of hemolysis assay and platelet adhesion, the chemical surface modified samples exhibited improved blood compatibility as compared to the pristine magnesium alloy. Furthermore, the chemical surface modified samples improved cytocompatibility to endothelial cells, the cells had better cell adhesion and proliferative profiles on the modified surfaces. Due to the excellent hydrophilicity, the NaOH-treated substrate displayed better blood compatibility and cytocompatibility to endothelial cells than that of HF-treated sample. It was considered that the method of the present study can be used for the surface modification of the magnesium alloy to enhance the corrosion resistance and biocompatibility.

show abstract

“…The non-obvious influence of PEO/HT on local alkalization of its surrounding environment may be critical for their utilization as implant considering that the cells are sensitive to a sharp change of their surrounding environment. Moreover, the increase of pH value during the degradation of magnesium is found to be the reason of hemolysis [37]. So the decreased local alkalization of PEO/HT is promising in practical application.…”

Section: Electrochemical Testsmentioning

confidence: 99%

In vitro degradation behavior and cytocompatibility of biodegradable AZ31 alloy with PEO/HT composite coating

Tian

Liu

Ding

2015

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

Hemolysis and cytotoxicity mechanisms of biodegradable magnesium and its alloys

Cited by 141 publications

References 39 publications

In vitro and in vivo cytocompatibility evaluation of biodegradable magnesium-based stents: a review

In vitro and in vivo cytocompatibility evaluation of biodegradable magnesium-based stents: a review

Improving Corrosion Resistance and Biocompatibility of Magnesium Alloy by Sodium Hydroxide and Hydrofluoric Acid Treatments

In vitro degradation behavior and cytocompatibility of biodegradable AZ31 alloy with PEO/HT composite coating

Contact Info

Product

Resources

About