Hemolymph Ecdysone and Electrolytes during the Molting Cycle of Crayfish: A Comparison of Natural Molts with Those Induced by Eyestalk Removal or Multiple Limb Autotomy

Wheatly, Michèle G.; Hart, Mary K.

doi:10.1086/physzool.68.4.30166346

Cited by 20 publications

(9 citation statements)

References 34 publications

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“…Immunodetectable ecdysone levels were low (generally below 50 pg/mg) through most of the molt cycle of the organism but transiently increased to approximately 150 pg/mg just prior to molting. This profile of ecdysone levels is consistent with that measured through the molt cycle of other crustaceans [21][22][23][24].…”

Section: Ecdysone Levels In Embryossupporting

confidence: 85%

Synergistic interaction of endocrine‐disrupting chemicals: Model development using an ecdysone receptor antagonist and a hormone synthesis inhibitor

LeBlanc

2004

Enviro Toxic and Chemistry

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Endocrine toxicants can interfere with hormone signaling through various mechanisms. Some of these mechanisms are interrelated in a manner that might result in synergistic interactions. Here we tested the hypothesis that combined exposure to chemicals that inhibit hormone synthesis and that function as hormone receptor antagonists would result in greater-than-additive toxicity. This hypothesis was tested by assessing the effects of the ecdysteroid-synthesis inhibitor fenarimol and the ecdysteroid receptor antagonist testosterone on ecdysteroid-regulated development in the crustacean Daphnia magna. Both compounds were individually characterized for effects on the development of isolated embryos. Fenarimol caused late developmental abnormalities, consistent with its effect on offspring-derived ecdysone in the maturing embryo. Testosterone interfered with both early and late development of embryos, consistent with its ability to inhibit ecdysone provided by maternal transfer (responsible for early developmental events) or de novo ecdysone synthesis (responsible for late developmental events). We predicted that, by decreasing endogenous levels of hormone, fenarimol would enhance the likelihood of testosterone binding to and inhibiting the ecdysone receptor. Indeed, fenarimol enhanced the toxicity of testosterone, while testosterone had no effect on the toxicity of fenarimol. Algorithms were developed to predict the toxicity of combinations of these two compounds based on independent joint action (IJA) alone as well as IJA with fenarimol-on-testosterone synergy (IJA+SYN). The IJA+SYN model was highly predictive of the experimentally determined combined effects of the two compounds. These results demonstrate that some endocrine toxicants can synergize, and this synergy can be accurately predicted.

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Section: Ecdysone Levels In Embryossupporting

confidence: 85%

Synergistic interaction of endocrine‐disrupting chemicals: Model development using an ecdysone receptor antagonist and a hormone synthesis inhibitor

LeBlanc

2004

Enviro Toxic and Chemistry

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“…The dry pellets were resuspended in borate buffer, and the ecdysteroid levels were determined by radioimmunoassay following the methods of Sonobe et al. () and Wheatly and Hart () with minor adjustments. The [23, 24‐ 3 H(N)] ecdysone (specific activity: 83.2 Ci/mmol; Perkin Elmer, USA) was used as the labeled ligand and the unlabeled ligand was 20‐hydroxyecdysone (Sigma‐Aldrich, USA).…”

Section: Methodsmentioning

confidence: 99%

A Role of Suppressor of Cytokine Signaling 2 in the Regulation of Ecdysteroid Signaling Pathway inProcambarus clarkii

Zhu

Dai

et al. 2016

J. Exp. Zool.

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A suppressor of cytokine signaling 2 (SOCS-2) homolog was identified from the red crayfish Procambarus clarkii. Phylogenic analysis showed that P. clarkii SOCS-2 (Pc-SOCS-2) was very similar to that of invertebrates. The sequence contains a central SH2 domain, a conserved SOCS box, and a variable N-terminal domain. Real-time polymerase chain reaction showed that Pc-SOCS-2 mRNA level was higher in muscle and heart than in the other tissues examined. A 40-kDa recombinant protein was successfully expressed and purified from Escherichia coli cells as confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Pc-SOCS-2 was significantly downregulated in the hepatopancreas after 20-hydroxyecdysone injection. Knockdown of Pc-SOCS-2 gene by small interfering RNA transfection in the hepatopancreas increased the expression levels of 20-hydroxyecdysone-responsive genes at 48 hr. Taken together, these results suggest that Pc-SOCS-2 is a negative regulator of ecdysteroid signaling transduction.

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“…A significant number of studies have examined the close relation between regeneration and the moult cycle in the crayfish and several other crustaceans 39,86,87 . In the proecdysial phase, the missing pereiopod of Orconectes obscurus is replaced 33 .…”

Section: Interactions Between Limb Regeneration and The Moult Cyclementioning

confidence: 99%

Biological insights into the rapid tissue regeneration of freshwater crayfish and crustaceans

Feleke

Bennett

Chen

et al. 2021

Cell Biochemistry & Function

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The freshwater crayfish is capable of regenerating limbs, following autotomy, injury and predation. In arthropod species, regeneration and moulting are two processes linked and strongly regulated by ecdysone. The regeneration of crayfish limbs is divided into wound healing, blastema formation, cellular reprogramming and tissue patterning. Limb blastema cells undergo proliferation, dedifferentiation and redifferentiation. A limb bud, containing folded segments of the regenerating limb, is encased within a cuticular sheath. The functional limb regenerates, in proecdysis, in two to three consecutive moults. Rapid tissue growth is regulated by hormones, limb nerves and local cells. The TGF‐β/activin signalling pathway has been determined in the crayfish, P. fallax f. virginalis, and is suggested as a potential regulator of tissue regeneration. In this review article, we discuss current understanding of tissue regeneration in the crayfish and various crustaceans. A thorough understanding of the cellular, genetic and molecular pathways of these biological processes is promising for the development of therapeutic applications for a wide array of diseases in regenerative medicine.

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Hemolymph Ecdysone and Electrolytes during the Molting Cycle of Crayfish: A Comparison of Natural Molts with Those Induced by Eyestalk Removal or Multiple Limb Autotomy

Cited by 20 publications

References 34 publications

Synergistic interaction of endocrine‐disrupting chemicals: Model development using an ecdysone receptor antagonist and a hormone synthesis inhibitor

Synergistic interaction of endocrine‐disrupting chemicals: Model development using an ecdysone receptor antagonist and a hormone synthesis inhibitor

A Role of Suppressor of Cytokine Signaling 2 in the Regulation of Ecdysteroid Signaling Pathway inProcambarus clarkii

Biological insights into the rapid tissue regeneration of freshwater crayfish and crustaceans

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