A Role of Suppressor of Cytokine Signaling 2 in the Regulation of Ecdysteroid Signaling Pathway in<i>Procambarus clarkii</i>

Zhu, Bing; Dai, Li-Shang; Yu, Yingying; Wang, Daojun; Peng, Tao; Liu, Chao-Liang

doi:10.1002/jez.2029

Cited by 9 publications

(5 citation statements)

References 43 publications

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“…The putative elongin B/C interaction residues in the SOCS box domain are indicated with green text. SOCS2 gene was composed of an ORF of 1,059 bp, encoding a polypeptide of 352 amino acid residues (Zhu et al 2016). The Pelteobagrus fulvidarco SOCS2 and rainbow trout Oncorhynchus mykiss SOCS2 was comprised of a 561 and 606 bp coding region, encoding polypeptides of 186 and 201 amino acids, respectively (Wang & Secombes 2008;Ye et al 2018).…”

Section: Molecular Characterization Of Tmsocs2mentioning

confidence: 99%

Molecular cloning and characterization of SOCS2 from the mealworm beetle Tenebrio molitor

Kim

Patnaik

et al. 2019

Entomological Research

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The suppressor of cytokine signaling (SOCS) family of proteins negatively regulate cytokine receptor signaling via the Janus kinase/signal transducer and activator of transcription (STAT) pathway. In this study, we discovered the SOCS2 homolog in the mealworm beetle Tenebrio molitor (TmSOCS2). The full‐length ORF sequence of TmSOCS2 was cloned, and expression pattern in the insect's developmental stages and tissues were investigated. TmSOCS2 encodes 254 amino acid polypeptide consisting of the conserved Src homology 2 (SH2) and SOCS box domains. The amino acid sequence of TmSOCS2 showed maximum 77% identity and minimum evolutionary distance of 0.228 with SOCS2 protein of another related beetle, Tribolium castaneum (TcSOCS2). In the phylogenetic tree, TmSOCS2 and TcSOCS2 formed a separate cluster showing evolutionary relatedness with the hemipteran bug Lygus hesperus SOCS2 (LhSOCS2). TmSOCS2 mRNA was maximally expressed in the egg stage of the insect. High relative expression of TmSOCS2 was observed in the Malpighian tubules during the insect's larval and adult stages. TmSOCS2 mRNA expression was significantly (P < 0.05) upregulated by E. coli challenge in the fat body tissue of T. molitor larvae. In hemocytes, early expression (at 3–6 h) of TmSOCS2 mRNA was observed after S. aureus and C. albicans challenge. Discovery and expression analysis of the SOCS2 homolog from the mealworm beetle T. molitor support transcript's putative involvement in the insect innate immune responses.

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Section: Molecular Characterization Of Tmsocs2mentioning

confidence: 99%

Molecular cloning and characterization of SOCS2 from the mealworm beetle Tenebrio molitor

Kim

Patnaik

et al. 2019

Entomological Research

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“…In the pacific oyster, Crassostrea gigas, three SOCS genes (SOCS2, SOCS5, and SOCS7) have been identified as putatively inducing NF-kB gene transcriptional activity. SOCS2 homologues with immune expression have also been identified in Ruditapes philippinarum [14], Haliotis discus [15], Eriocheir sinensis [16], Procambarus clarkii [17], and Litopenaeus vannamei [18]. Further, direct functional evidence suggests that SOCS6 has a role in the activation of the NF-kB signaling pathway in E. sinensis [19].…”

Section: Introductionmentioning

confidence: 96%

Molecular Cloning and Expression Characterization of Three Suppressors of Cytokine Signaling Genes (SOCS5, SOCS6, SOCS7) from the Mealworm Beetle, <em>Tenebrio molitor</em>

Patnaik¹,

Kim²,

Jo³

et al. 2019

Preprint

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Suppressors of cytokine signaling (SOCS) influence cytokine and growth factor signaling by negatively regulating the JAK-STAT pathway. This maintains homeostasis during host immune response. However, functional characterization of SOCS family members in invertebrates is limited. In this study, we discovered the Type-I subfamily of the SOCS genes in the mealworm beetle, T. molitor. The full-length ORFs of TmSOCS5, TmSOCS6, and TmSOCS7 consisted of 1,389, 897 and 1,458 nucleotides, encoding polypeptides of 462, 297 and 485 amino acids, respectively, The C-terminal region of TmSOCS was highly conserved in the SH2 and SOCS box domains. Phylogenetic analysis revealed that the three SOCS genes clustered within the same sub-family and the highest amino acid identity was with the Tribolium castaneum SOCS genes (TcSOCS). While the expression of TmSOCS5 and TmSOCS6 was low in larval, pupal, and adult stages of the insect, TmSOCS7 showed higher expression. The expression of TmSOCS5 and TmSOCS6 was higher in larval hemocytes and adult ovary. The microbes expressed the three TmSOCS genes to varying degrees. C. albicans elicited the strongest response in the host with highest 15-fold expression in TmSOCS7 3 h post-inoculation.  Collectively, these data suggest that the Type I TmSOCS could play a role in eliciting host immunity.

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“…Additionally, in the Pacific oyster (Crassostrea gigas), three SOCS genes (SOCS2, SOCS5, and SOCS7) have been identified, exhibiting potential for the regulation of NF-κB transcription [17]. Furthermore, the expression and functional implications of SOCS2 (which belongs to the type II SOCS family) in the innate immune responses of red swamp crayfish and whiteleg shrimp (Procambarus clarkii and L. vannamei) have been investigated following lipopolysaccharide (LPS) or bacterial challenges [18,19]. These studies conveyed the importance of SOCS genes in the immune defense mechanisms of crustaceans.…”

Section: Introductionmentioning

confidence: 99%

Molecular Cloning and Gene Expression of Type I Suppressors of Cytokine Signaling 6 and 7 (SOCS6 and SOCS7) in Whiteleg Shrimp (Litopenaeus vannamei)

Cwr

Prabhatha

Lee

et al. 2023

Fishes

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Suppressor of cytokine signaling (SOCS) genes are essential negative regulators that modulate cytokine signaling and play key roles in numerous biological processes, including immune responses. In this study, we cloned the complementary DNA (cDNA) sequences of two SOCS genes, designated as LvSOCS6 and LvSOCS7, from the whiteleg shrimp, Litopenaeus vannamei. LvSOCS6 encoded a polypeptide of 463 amino acids (aas), spanning 1392 base pairs (bps), while LvSOCS7 encoded a significantly larger polypeptide of 955 aas, encompassing 2868 bps. Both LvSOCS proteins exhibited conserved domains associated with SOCS, including a centralized Src homology 2 (SH2) domain and a C-terminal SOCS box. Phylogenetic analysis revealed that the deduced aa sequences of LvSOCS6 and LvSOCS7 clustered within the invertebrate type I SOCS family, indicating their evolutionary relatedness. Tissue distribution analysis demonstrated ubiquitous expression of both LvSOCS genes across all examined tissues, with LvSOCS6 showing heightened expression in the gills and LvSOCS7 in the gills and stomach. Notably, mRNA expression patterns of LvSOCS genes following LPS and poly (I:C) stimulations exhibited significant upregulations, while PGN stimulation yielded incongruous results across the examined tissues. Interestingly, concurrent with the diminished expression of LvSOCS6 and LvSOCS7, there was significant elevation in mRNA expression levels of LvSTAT, a vital component of the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway. These findings shed light on the potential involvement of the newly discovered type I SOCS genes in regulating the JAK/STAT pathways and playing pivotal roles in orchestrating the innate immune responses in L. vannamei defense mechanisms.

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A Role of Suppressor of Cytokine Signaling 2 in the Regulation of Ecdysteroid Signaling Pathway inProcambarus clarkii

Cited by 9 publications

References 43 publications

Molecular cloning and characterization of SOCS2 from the mealworm beetle Tenebrio molitor

Molecular cloning and characterization of SOCS2 from the mealworm beetle Tenebrio molitor

Molecular Cloning and Expression Characterization of Three Suppressors of Cytokine Signaling Genes (SOCS5, SOCS6, SOCS7) from the Mealworm Beetle, <em>Tenebrio molitor</em>

Molecular Cloning and Gene Expression of Type I Suppressors of Cytokine Signaling 6 and 7 (SOCS6 and SOCS7) in Whiteleg Shrimp (Litopenaeus vannamei)

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