Hemoglobin detection using curcumin nanoparticles as a colorimetric chemosensor

Abstract

This article presents a simple and efficient measurement system for quantitative sensing of blood hemoglobin (Hgb) using curcumin nanoparticle (CURNs). The Hgb monitoring is based on CURNs aggregation in the presence of Hgb, which leads to a decrease in the absorption intensity of CURNs. In this study the analytical signal for the measurement of blood Hgb concentration was defined as the difference in absorption intensity of the CURNs in the absence and presence of Hgb. The method was optimized to enhance the selectivity and sensitivity of the method by investigating the effect of variables such as pH of the sample solution, buffer concentration, required amount of CURNs, equilibrium time and tolerance limit of various probable interferences. Under optimum conditions, the difference in absorption intensity of the CURNs was linearly proportional to the concentration of Hgb in two ranges of 1-40 µg mL -1 and 150-1200 µg mL -1 with a detection limit of 0.1 µg mL -1 . The relative standard deviation for ten replicate measurements of 20 µg mL -1 and 600 µg mL -1 of Hgb was 3.6% and 3.1% respectively.The sensing method was successfully applied for the measurement of Hgb in human blood with satisfactory results.

Boron and nitrogen co‐doped carbon dots as a sensitive fluorescent probe for the detection of curcumin

“…Figure 9 shows the absorption spectrum of curcumin, which has a significant spectral overlap with the fluorescence excitation or emission spectra of BNCDs, suggesting that the possible fluorescence quenching mechanisms between curcumin and BNCDs arises from the IFE. [40] Figure S5 displays the UV-vis absorption spectra of BNCDs (4), curcumin (1)(2)(3) and BNCDs with different concentrations of curcumin (8)(9)(10). Curve 5 was obtained by deducting the curve 1 from curve 8. and BNCDs has not occurred.…”

A carbon dots based fluorescent probe for selective and sensitive detection of hemoglobin

“…Therefore, the easy and accurate quantitative analysis of HGB in clinical diagnostics is essential to assess the extent of diseases and to evaluate various intervention programs aimed at control of diseases. Recently, numerous attempts have been made to detect HGB including colorimetric approaches [17,18], spectrophotometric methods [19][20][21][22][23], electrochemical measurements [24,25], and chemiluminescence determination [26]. Unfortunately, the majority of the aforementioned Page 6 of 30 A c c e p t e d M a n u s c r i p t 4 techniques suffer from the application of toxic reagents, expensive equipment, complicated sample pretreatment, and relatively higher detection limit of HGB.…”

Highly photoluminescent and temperature-sensitive P, N, B-co-doped carbon quantum dots and their highly sensitive recognition for curcumin

“…As a biologically active constituent of turmeric, 1 curcumin exhibits diverse biological and pharmacological activities such as anti-inammatory, 2 antibacterial, 3 hepatoprotective, 4 anticarcinogenic, 5 antioxidant 6,7 and antidyspeptic 8 properties. As a result, curcumin has been widely applied in pharmacology for many years.…”