Hemocytic immune responses triggered by CpG ODNs in shrimp Litopenaeus vannamei

Sun, Rui; Qiu, Limei; Yue, Feng; Wang, Lingling; Li, Rui; Zhou, Zhi; Zhang, Huan; Song, Linsheng

doi:10.1016/j.fsi.2012.09.016

Cited by 30 publications

(15 citation statements)

References 34 publications

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“…In the past decades, the shrimp aquaculture suffered from the frequent threatens of various diseases. The white spot syndrome virus (WSSV) is one of the most prevalent and severe pathogens in shrimp aquaculture [23,24]. It has been reported that the glucose level increased significantly in hemolymph of L. vannamei after WSSV infection [24,25], and two LvCHHs (ADL27417, which was previously suggested as type I CHH and designated as LvCHH I here, and AAN86057 which was suggested as type II CHH and designated as LvCHH II here) with a single or double crustacean neurohormone domain have been identified in shrimp.…”

Section: Introductionmentioning

confidence: 99%

“…The white spot syndrome virus (WSSV) is one of the most prevalent and severe pathogens in shrimp aquaculture [23,24]. It has been reported that the glucose level increased significantly in hemolymph of L. vannamei after WSSV infection [24,25], and two LvCHHs (ADL27417, which was previously suggested as type I CHH and designated as LvCHH I here, and AAN86057 which was suggested as type II CHH and designated as LvCHH II here) with a single or double crustacean neurohormone domain have been identified in shrimp. The purposes of this study were to (1) survey the expression patterns of LvCHH I and LvCHH II during WSSV infection, (2) verify their modulation role in glucose metabolism, (3) investigate the potential immunomodulation role of LvCHHs by directly binding to the shrimp hemocytes, aiming to provide new hints for the immunomodulation role of glucose-related hormones in invertebrates.…”

Section: Introductionmentioning

confidence: 99%

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Crustacean hyperglycemic hormones directly modulate the immune response of hemocytes in shrimp Litopenaeus vannamei

Wang

Chen

et al. 2017

Fish & Shellfish Immunology

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A robust immune response against invading pathogens is crucial for host to survive, which depends greatly on the well balance of metabolism. Increasing evidence has indicated that some metabolic hormones, such as insulin, could modulate immune responses directly. Crustacean hyperglycemic hormone (CHH) family is a group of ecdysozoans-specific peptide hormone involved in glucose metabolism and other biological events. In the present study, two members of CHH family (designated as LvCHH I and LvCHH II) in shrimp Litopenaeus vannamei with one and two crustacean neurohormone domains respectively were chosen to investigate their putative modulatory roles in both glucose metabolism and immune response. LvCHH I and LvCHH II were both expressed in the sinus gland and lamina ganglionalis of eyestalks and were significantly induced after white spot syndrome virus (WSSV) infection. Meanwhile, significant increases of hemolymph glucose levels were observed in shrimp at 12 and 24 h after WSSV infection while the glucose inside the hemocytes decreased at 6 h and then increased at 12 h. Gain-of-function of rLvCHHs was subsequently conducted in vivo by injecting the recombinant proteins (rLvCHH I and rLvCHH II). The hemolymph glucose increased significantly from 0.5 h to 3 h after the shrimps received an injection of rLvCHH I, while it decreased at 0.5 h and increased afterward at 3 h post rLvCHH II injection. At the meantime, significant decreases of reactive oxygen species level in hemocytes were observed at 3 h and 6 h post rLvCHH I injection, while it remained unchanged in rLvCHH II injection group. rLvCHH I and rLvCHH II could bind to the cytomembrane of primary shrimp hemocytes in vitro, and the expressions of superoxide dismutase and LvRelish increased when the hemocytes were incubated with rLvCHH I for 3 h. Meanwhile, the expression of antimicrobial peptides, crustin and penaeidin-4, were also induced by rLvCHH I and rLvCHH II. These results demonstrated that host immune response, in addition to glucose metabolism, could be directly modulated by LvCHH family, and the present study provided new insights into the immunomodulation role of metabolic hormones in invertebrate.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Crustacean hyperglycemic hormones directly modulate the immune response of hemocytes in shrimp Litopenaeus vannamei

Wang

Chen

et al. 2017

Fish & Shellfish Immunology

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“…; Sun et al . ). By committing suicide, the virus can no longer replicate and the dead haemocytes that contain fragmented viruses are destroyed by neighbouring haemocytes.…”

Section: Discussionmentioning

confidence: 97%

“…The molecular mechanism involved in CpG ODNs and viral DNA-mediated cell death is unknown. It is very well possible that CpG ODNs and viral DNA are sensed by Toll-like receptors and then activate Toll and JAK/STAT signalling pathway, resulting in the death of cells as earlier been shown for mammal cells (Liang et al 2010;Sun et al 2013). By committing suicide, the virus can no longer replicate and the dead haemocytes that contain fragmented viruses are destroyed by neighbouring haemocytes.…”

Section: Discussionmentioning

confidence: 99%

Kinetic analysis of internalization of white spot syndrome virus by haemocyte subpopulations of penaeid shrimp, Litopenaeus vannamei (Boone), and the outcome for virus and cell

Tuan

Gryse

Thuong

et al. 2016

Journal of Fish Diseases

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Little is known about the innate antiviral defence of shrimp haemocytes. In this context, the haemocytes of penaeid shrimp Litopenaeus vannamei (Boone) were separated by iodixanol density gradient centrifugation into five subpopulations (sub): sub 1 (hyalinocytes), sub 2 and 3 (prohyalinocytes), sub 4 (semigranulocytes) and sub 5 (granulocytes) and exposed to beads, white spot syndrome virus (WSSV) and ultraviolet (UV)-killed WSSV. In a first experiment, the uptake of beads, white spot syndrome virus (WSSV) and UV-killed WSSV by these different haemocyte subpopulations was investigated using confocal microscopy. Only haemocytes of sub 1, 4 and 5 were internalizing beads, WSSV and UV-killed WSSV. Beads were engulfed by a much larger percentage of cells (91.2 in sub 1; 84.1 in sub 4 and 58.1 in sub 5) compared to WSSV (9.6 in sub 1; 10.5 in sub 4 and 7.9 in sub 5) and UV-killed WSSV (12.9 in sub 1; 13.3 in sub 4; and 11.8 in sub 5). In a second experiment, it was shown that upon internalization, WSS virions lost their envelope most probably by fusion with the cellular membrane of the endosome (starting between 30 and 60 min post-inoculation) and that afterwards the capsid started to become disintegrated (from 360 min post-inoculation). Expression of new viral proteins was not observed. Incubation of haemocyte subpopulations with WSSV but not with UV-killed WSSV and polystyrene beads resulted in a significant drop in haemocyte viability. To find the underlying mechanism, a third experiment was performed in which haemocyte subpopulations were exposed to a short WSSV DNA fragment (VP19) and CpG ODNs. These small DNA fragments induced cell death. In conclusion, WSSV is efficiently internalized by hyalinocytes, semigranulocytes and granulocytes, after which the virus loses its envelope; as soon as the capsids start to disintegrate, cell death is activated, which in part may be explained by the exposure of viral DNA to cellular-sensing molecules.

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“…The most realistic approach to combat diseases is combining good husbandry and good feed with the use of prophylactic agents, including immunostimulants and probiotics [83-85]. …”

Section: Priority Areas and Major Randd Progress Of Marine Biotechnologmentioning

confidence: 99%

Recent Major Advances of Biotechnology and Sustainable Aquaculture in China

Xiang¹

2015

CBIOT

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Background: Global aquaculture production has increased continuously over the last five decades, and particularly in China. Its aquaculture has become the fastest growing and most efficient agri-sector, with production accounting for more than 70% of the world’s aquaculture output. In the new century, with serious challenges regarding population, resources and the environment, China has been working to develop high-quality, effective, healthy, and sustainable blue agriculture through the application of modern biotechnology. Sound knowledge related to the biology and ecology of aquatic organisms has laid a solid foundation and provided the innovation and technology for rapid development of the aquaculture industry. Marine biotechnology, which is enabling solutions for ocean productivity and sustainability, has been promoted since the last decades of the 20th Century in China.Objective: In this article, priority areas of research, mainly genetic breeding, omics studies, novel production systems, biosecurity, bioprocesses and biorefinery, as well as the major progress of marine biotechnology R&D in China are reviewed.Conclusion: Current innovative achievements in China are not enough and the level and frequency of academic advancements must be improved. International cooperation and assistance remain crucial for the success of marine biotechnology.

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Hemocytic immune responses triggered by CpG ODNs in shrimp Litopenaeus vannamei

Cited by 30 publications

References 34 publications

Crustacean hyperglycemic hormones directly modulate the immune response of hemocytes in shrimp Litopenaeus vannamei

Crustacean hyperglycemic hormones directly modulate the immune response of hemocytes in shrimp Litopenaeus vannamei

Kinetic analysis of internalization of white spot syndrome virus by haemocyte subpopulations of penaeid shrimp, Litopenaeus vannamei (Boone), and the outcome for virus and cell

Recent Major Advances of Biotechnology and Sustainable Aquaculture in China

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