Hemocyte production in Biomphalaria glabrata snails after exposure to different Schistosoma mansoni infection protocols

Santos, Daniela Soares; Santos, Marina Pereira Queiroz dos; Rodrigues, Izabel Raimunda de Carvalho

doi:10.5123/s2176-62232011000200005

Cited by 7 publications

(7 citation statements)

References 22 publications

(18 reference statements)

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“…Eissa et al 10 understand the processes leading to parasitic infection, and to determine the processes that make the snails resistant or susceptible to the parasite infection 26 . Hemocytes have the ability to encapsulate foreign organisms as the result of the adaptations of their cytoskeletal mobility and their plasma membranes 27 . In the present study, the evaluation of hemocytes denoted that S. mansoni infected snails had higher significant increase in hemocyte production comparable with treated-infected snail groups with miltefosine and artemether.…”

Section: Resultsmentioning

confidence: 99%

Sublethal toxicity of miltefosine and artemether to molecular aspect of Biomphalaria alexandrina snails

2019

jcbsc

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The present study was performed to elucidate the genotoxicity effect of sublethal concentration of miltefosine (8.20 ppm) as a synthetic molluscicidal agent and artemether (11.04 ppm) as plant molluscicidal agent against Biomphalaria alexandrina snails; vectors for transmitting Schistosoma mansoni infection to human. DNA changes of hemocytes of treated snails were studied by comet assay and the overall results showed that the miltefosine had more toxic effect than artemether against snails. The results also, demonstrated that both examined drugs induced a significant decrease in hemocytes count comparable with their corresponding controls. The drugs negatively affect DNA of hemocytes and appear as a potential inhibitor of DNA synthesis in snails. Consequently, in conclusion, the tested materials could be ranked as beneficial promising molluscicidal agents for the control program of schistosomiasis.

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Section: Resultsmentioning

confidence: 99%

Sublethal toxicity of miltefosine and artemether to molecular aspect of Biomphalaria alexandrina snails

2019

jcbsc

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“…Evaluation of the number of hemocytes in the hemolymph is usually expressed as the THC, while morphological differences among hemocytes is expressed as the DHC (Barracco et al, 1993;Donaghy et al, 2009;Comesana et al, 2012). glabrata, where the THC was significantly reduced at 5 h after infection with S. mansoni (Bezerra et al, 1997;Santos et al, 2011) and reduced at 4 h up to 72 h after infection with A.…”

Section: Discussionmentioning

confidence: 99%

“…Experimental studies have emphasized the positive effect of parasitic infections on the number of hemocytes in the hemolymph of various species (McReath et al, 1982;Bezerra et al, 1997;Ordas et al, 2000;Oliveira et al, 2010;Santos et al, 2011;Barcante et al, 2012). Furthermore, differences in the cell proportion occur often.…”

Section: Introductionmentioning

confidence: 99%

“…At the onset of infection, the small cells (agranulocytes) change into granulocytes, which have a large size, numerous cytoplasmic inclusions and long pseudopodia (van der Knaap and Meuleman, 1986;van der Knaap et al, 1993;Gorbushin and Iakovleva, 2006). These granulocytes are effector cells that migrate to the infection site, thus the number of granulocytes in the hemolymph decreases (Bezerra et al, 1997;Santos et al, 2011;Barcante et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Hemocyte subpopulation changes in Bithynia snails infected with Opisthorchis viverrini in Thailand.

Suwannatrai

Donthaisong

et al. 2019

Preprint

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The Bithynia snails of B. funiculata, B. siamensis siamensis and B. siamensis goniomphalos, are first intermediate hosts of Opisthorchis viverrini. The success of parasitic infection in snails is related to the host species and efficiency of their internal defense system. Parasitic infections in snails exhibited significant variations in the number of hemocytes in hemolymph. This study investigated hemocyte counts in Bithynia snails for various durations of O. viverrini infection. The three species/subspecies snails were infected with O. viverriniand hemocytes were counted at post-exposed periods of 2, 4, 6, 12, 24, 48, 96 h, and 1, 4 and 8 wk to evaluate the hemocyte response to the infection. Three major hemocyte morphotypes, namely, agranulocytes, semigranulocytes and granulocytes, were observed. After infection, the differential hemocyte morphotype counts in all species/subspecies significantly increased in granulocytes at 2 h (p < 0.05) and decreased in semigranulocytes and agranulocytes at 2 to 4 h (p < 0.05). The total hemocyte counts were significant increased at 2 h after exposure in B. s. siamensis and B. funiculata (p < 0.05). Additionally, the number of hemocytes was reduced after exposure at 4, 6 and 12 h in B. s. goniomphalos and at 12, 24 and 48 h in B. funiculata (p < 0.05). From 96 h to 8 wk, the number of hemocytes was reestablished in the hemolymph, indicating that defensive cells in the host species have different mechanisms for their susceptibility or resistance to parasitic infections. Further studies on molecular functions will be done.

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“…A maioria dos estudos com hemócitos de gastrópodes referem-se a espécies dos gêneros Biomphalaria (Preston, 1910) e Lymnaea Lamarck, 1799 sob condições, ou não, de infecção (Müller, 1956;SMinia, 1972;harriS, 1975;yoShino, 1976;cheng & guida, 1980;loverde et al, 1982;Jeong et al, 1983;JoKy et al, 1983;SMinia et al, 1983;Barraco et al, 1993;BeZerra et al, 1997BeZerra et al, , 2003Matricon-gondran & letocart, 1999;ruSSo & lagadic, 2004;BarBoSa et al, 2006;SantoS & diniZ, 2009;SantoS et al, 2011;cavalcanti et al, 2012). O conhecimento sobre o sistema imunológico dos gastrópodes é importante, principalmente o conhecimento dos hemócitos de caracóis não infectados, para que em futuros estudos, seja possível avaliar a ação do parasitismo sobre o sistema de defesa do hospedeiro.…”

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Hemócitos de Bradybaena similaris e Megalobulimus abbreviatus (Gastropoda, Stylommatophora)

Rohr

Amato

2014

Iheringia, Sér. Zool.

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ABSTRACT. Hemocytes of Bradybaena similaris and Megalobulimus abbreviatus (Gastropoda, Stylommatophora). Hemocytes act in the defense system against invading organisms, foreign particles aiding the recognition of what is own to the body of gastropods and what is not. Information and studies on the hemocytes in species of mollusks healthy (no infections), especially in Bradybaena similaris (Fèrussac, 1821) and Megalobulimus abbreviatus (Bequaert, 1948) are scarce. Therefore, this work aims at characterization and quantification of hemocytes present in the hemolymph of these two species. In this work three cell types were identified in the hemolymph of both species: round cells, hyalinocytes and granulocytes. The three types of hemocytes were measured, and the average of total diameter and the nucleus for each was calculated. On B. similaris, the average diameter of round cells was 10.7 µm, of hyalinocytes was 20 µm and of granulocytes was 25.4 µm. On M. abbreviatus, the average diameter of round cells was 11.7 µm, of hyalinocytes was 21.5 µm and of granulocytes was 30.5 µm. Although the hyalinocytes have similar averages between B. similaris and M. abbreviatus, the cells were demonstrated significant differences in their total diameter and size of the nucleus (p<0.0001). The average density of cells per ml without distinction of cellular type was 197,813 cells/ml for M. abbreviatus, and 416,333 cells/ml for B. similaris. The most frequent hemocytes in M. abbreviatus and B. similaris were hyalinocytes, unlike other gastropods. KEYWORDS.Granulocytes, hyalinocytes, Mollusca, round cells. RESUMO.Os hemócitos atuam no sistema de defesa contra organismos invasores e partículas estranhas, auxiliando o reconhecimento do que é próprio do corpo dos grastrópodes e o que não é. São escassas as informações e estudos sobre os hemócitos em espécies de moluscos saudáveis (sem infecções), principalmente em Bradybaena similaris (Fèrussac, 1821) and Megalobulimus abbreviatus (Bequaert, 1948). Portanto, este trabalho tem como objetivos a caracterização e quantificação dos hemócitos presentes na hemolinfa destas duas espécies. Neste trabalho, foram identificados três tipos celulares na hemolinfa de ambas espécies: as células redondas, hialinócitos e granulócitos. Os três tipos de hemócitos foram medidos e foi calculada a média do diâmetro total e do núcleo para cada um deles. Para B. similaris, o diâmetro médio das células redondas foi de 10,7 µm, dos hialinócitos foi de 20 µm e dos granulócitos de 25,4 µm. Para M. abbreviatus, o diâmetro médio foi de 11,7 µm para as células redondas, de 21,5 µm para os hialinócitos e de 30,5 µm para os granulócitos. Embora os hialinócitos possuam médias parecidas entre B. similaris e M. abbreviatus, foram detectadas diferenças significativas do diâmetro celular total e diâmetro do núcleo (p<0,0001) dessas células entre as espécies estudadas. A densidade média de células por ml, sem distinção de tipo celular foi de 197.813 células/ml para M. abbreviatus, e de 416.333 células/ml para B. similaris. ...

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Hemocyte production in Biomphalaria glabrata snails after exposure to different Schistosoma mansoni infection protocols

Cited by 7 publications

References 22 publications

Sublethal toxicity of miltefosine and artemether to molecular aspect of Biomphalaria alexandrina snails

Sublethal toxicity of miltefosine and artemether to molecular aspect of Biomphalaria alexandrina snails

Hemocyte subpopulation changes in Bithynia snails infected with Opisthorchis viverrini in Thailand.

Hemócitos de Bradybaena similaris e Megalobulimus abbreviatus (Gastropoda, Stylommatophora)

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