Hemocompatibility of different burn wound dressings

Abstract

A variety of wound dressing are available for burns. Furthermore, although their impacts on wound healing have been studied sufficiently, their effects on blood remain unclear. Meanwhile, this aspect is extremely important, since blood interacts with the wound dressing, especially in extensive burn injuries. Therefore, the aim of this study is to evaluate the hemocompatibility and immunogenicity of different burn wound dressings. Accordingly, human whole blood (n = 5) was anticoagulated with heparin, treated with different wound dressings and incubated at 37 C for 30 minutes. Different parameters for coagulation and hemocompatibility were evaluated before and after incubation. Consequently, Jelonet, Xenoderm, and Matriderm showed higher TAT-III concentrations, Jelonet, Xenoderm, EZ Derm, and Matriderm were higher β-thromboglobulin; EZ Derm and Burntec showed higher SC5b-9 concentrations after incubation with whole blood. Our ex vivo study provided initial insights into the hemocompatibility and immunogenicity of different burn wound dressings. Moreover, Xenografts (Xenoderm and EZ Derm), Jelonet and Matriderm showed a hemostyptic effect, while EZ Derm and Burntec activated the complement system. Therefore, further studies must be conducted to analyze the possible effects in vivo. Hemocompatibility of different burn wound dressingsDenzinger et al.

Complement activation at the interface of wound dressings and blood does not influence keratinocyte migration/proliferation in vitro

“…As reported in our previous study, 3 only the plasma gained from the incubation of blood with sample D ( EZ Derm ) showed a significantly increased SC5b‐9 concentration (1428 ± 278.7 ng/mL) indicating a significant activation of the whole complement system. However, the plasma processed from incubation of blood with sample G ( Burntec ) nonetheless revealed a noticeable higher SC5b9 concentration (1283 ± 387.9 ng/mL).…”

“…The complement system consists of a tightly regulated network of proteins and its activation results in the formation of membrane attack complex C5b‐9. Recently, we reported that some wound dressings caused complement activation at the interface of wound dressing and whole blood 3 …”

“…Human whole blood was anticoagulated with heparine and incubated with wound dressings Jelonet (A), Epigard (B), Xenodem (C), EZ Derm (D), Biobrane (E), Matriderm (F), Burntec (G), and Suprathel (H) as previously described 3 . Since the contact of blood with the test tubes is likely to induce complement activation as well, freshly drawn blood (= BL, baseline) and blood incubated without samples (= control) both served as control groups.…”

“…Since the contact of blood with the test tubes is likely to induce complement activation as well, freshly drawn blood (= BL, baseline) and blood incubated without samples (= control) both served as control groups. All samples were processed for subsequent SC5b‐9 quantification 3 . The blood samples were transferred into monovettes containing EDTA (2.7 mL) and centrifuged for 20 minutes at 2500 g and 4°C.…”

“…Besides growth factors, also complement factors seem to play an important role in the wound healing process 2 . Recently, we reported that some wound dressings caused complement activation at the interface of dressing and whole blood 3 . In this study, we provided new insights into the interaction of the body's epidermis with the wound pad and present an in vitro setting to assess the hemocompatibility of wound dressings.…”