Hematopoietic stem cells in co-culture with mesenchymal stromal cells - modeling the niche compartments in vitro

Jing, Duohui; Fonseca, Ana Violeta; Alakel, Nael; Fierro, Fernando A.; Müller, Karl H.; Bornhäuser, Martin; Ehninger, Gerhard; Corbeil, Denis; Ordemann, Rainer

doi:10.3324/haematol.2009.010736

Cited by 196 publications

(170 citation statements)

References 38 publications

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“…Many groups have shown that co-culture of leukemic cells with BMSC or OB provides a survival advantage when challenged with chemotherapy agents 9,10,[12][13][14][19][20][21] . Work modeling normal immature CD34+ hematopoietic cells in coculture with mesenchymal stem cells (MSC) revealed that hematopoietic cells will interact with the adherent monolayer of MSCs to form three distinct spatial populations of hematopoietic cells 22,23 . Proliferation and differentiation of the CD34+ cells was effected relative to their location within the co-culture 22 .…”

Section: Discussionmentioning

confidence: 99%

“…Work modeling normal immature CD34+ hematopoietic cells in coculture with mesenchymal stem cells (MSC) revealed that hematopoietic cells will interact with the adherent monolayer of MSCs to form three distinct spatial populations of hematopoietic cells 22,23 . Proliferation and differentiation of the CD34+ cells was effected relative to their location within the co-culture 22 . We have expanded on this observation to test questions related to bone marrow microenvironment stromal cell support of a resistant population of tumor cells within a 2D co-culture and its isolation for downstream analysis.…”

Section: Discussionmentioning

confidence: 99%

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Modeling Chemotherapy Resistant Leukemia <em>In Vitro</em>

Slone

Moses

Evans

et al. 2016

JoVE

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It is well established that the bone marrow microenvironment provides a unique site of sanctuary for hematopoietic diseases that both initiate and progress in this site. The model presented in the current report utilizes human primary bone marrow stromal cells and osteoblasts as two representative cell types from the marrow niche that influence tumor cell phenotype. The in vitro co-culture conditions described for human leukemic cells with these primary niche components support the generation of a chemoresistant subpopulation of tumor cells that can be efficiently recovered from culture for analysis by diverse techniques. A strict feeding schedule to prevent nutrient fluxes followed by gel type 10 cross-linked dextran (G10) particles recovery of the population of tumor cells that have migrated beneath the adherent bone marrow stromal cells (BMSC) or osteoblasts (OB) generating a "phase dim" (PD) population of tumor cells, provides a consistent source of purified therapy resistant leukemic cells. This clinically relevant population of tumor cells can be evaluated by standard methods to investigate apoptotic, metabolic, and cell cycle regulatory pathways as well as providing a more rigorous target in which to test novel therapeutic strategies prior to pre-clinical investigations targeted at minimal residual disease. Video LinkThe video component of this article can be found at

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Modeling Chemotherapy Resistant Leukemia <em>In Vitro</em>

Slone

Moses

Evans

et al. 2016

JoVE

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“…However, MSC have also been shown to be effective when sourced from other tissues including human placenta , umbilical cord (Bakhshi et al, 2008, Huang et al, 2007, Wang et al, 2004 and adipose tissue (Nakao et al, 2010). Recent papers have demonstrated that many of the specific cell-cell interactions between HSC and stromal cells are critical and may be essential for HSC regulation both in vivo (Steiner et al, 2009) and in vitro (Jing et al, 2010, Song et al, 2010, Wagner et al, 2008, Wagner et al, 2007, Wein et al, 2010. Indeed, a majority of studies have shown that cell-cell contact between HSC and MSC is essential for their ex vivo expansion.…”

Section: Preclinical and Clinical Studies Using Cord Blood Hscmentioning

confidence: 99%

Exploring the Human Term Placenta as a Novel Source for Stem Cells and their Application in the Clinic

Heazlewood¹,

Cook²,

Ilić³

et al. 2012

Recent Advances in Research on the Human Placenta

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“…Both of the mechanisms can be involved. The authors succeeded showing that predominantly CD34 + CD38 -HSCs migrate across the MSCs layers [57].…”

Section: Cd38mentioning

confidence: 91%

Structural-functional organisation of the bone marrow hematopoietic stem cells niches

Nikolskaya¹,

Butenko²

2016

JCOT

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This article focuses on (1) the analysis of the structural-functional organization of bone marrow niches of the hematopoietic stem cells, (2) the role of the intercellular contact interactions and humoral regulation factors in these niches, in particular CXCL12, SCF and TGFβ,and (3) KEYWORDS: bone marrow, hematopoietic stem cell niche, multipotent stromal cells, hematopoiesisMaksimov and finally established at the end of the past century, it is not only the number but also entire known diversity of cell elements of immune and blood systems originate from only one type of the progenitor elements -the HSCs (unitary theory) [2].Life force and productivity of the HSCs is proved, for example, by the fact that 99.9 % of all blood-generating cells, HSCs included, in the irradiated mice can perish, and the surviving stem cells rapidly renew blood formation, including proper population as well as all types of the committed progenitors [3]. Along same sequence, the fact of non-exhaustion of blood formation after repeated damaging exposures is explained by the presence and functioning of the HSCs [1].The specialized cells of adult and fetal periods of ontogenesis, residing in the bone marrow (BM) in the quiescent state and capable, together with blood-forming microenvironment, to realize underlying programs for self-maintenance and multipotency allowing them (with maintained amount of HSCs) to release necessary amount of cells into differentiation along various directions that ultimately leads to the formation of all forms of blood elements, including immune system cells. It should be noted that the self-maintenance is not identical to the immortality. Stem cells are characterized by sufficiently long life, commensurable with entire organism's life course. Several genetic regulatory programs have been established which are of great importance in the process of HSCs self-maintenance. It is also known that stem cells in various tissues are controlled by common genetic programs maintaining their nature [4,5].It is theorized that stem cells are the clonogenic units which under certain conditions can be increased in their number at the expense Immune system is the structural-functional and interrelated commonness of the hematopoietic and stromal cells. Different in their origin, structure and functions, the populations and subpopulations of lymphoid and myeloid cells make a complete wholeness in the definite tissues and organs where they closely cooperate with non-hematopoietic elements. Constant and strictly regulated intensity of hematopoiesis with production of various cell types is also conditioned and controlled by cooperation of the hematopoietic and stromal cells. Timely repopulation of the immune system with hematopoietic cells-precursors, lymphocytes, leucocytes and stromal cells, newly maturing in the bone marrow, is the main condition for effective immune system functioning. The number of cells formed per time unit is plentiful. According to the given data, nearly 10 11 neutrophils (about 100 g of mass) per...

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Hematopoietic stem cells in co-culture with mesenchymal stromal cells - modeling the niche compartments in vitro

Cited by 196 publications

References 38 publications

Modeling Chemotherapy Resistant Leukemia <em>In Vitro</em>

Modeling Chemotherapy Resistant Leukemia <em>In Vitro</em>

Exploring the Human Term Placenta as a Novel Source for Stem Cells and their Application in the Clinic

Structural-functional organisation of the bone marrow hematopoietic stem cells niches

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