Exploring the Human Term Placenta as a Novel Source for Stem Cells and their Application in the Clinic

Heazlewood, Celena; Cook, Matthew; Ilić, Nina; Atkinson, Kerry

doi:10.5772/33976

Cited by 4 publications

(6 citation statements)

References 94 publications

(108 reference statements)

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“…This equated to a mean doubling time for haematopoietic cells on MSCs and osteoblasts of 17.5 h and 15.9 h respectively. The expansion appeared to be MSC- or osteoblast-specific, since LSKs co-cultured in the presence of adipocyte-differentiated MSCs showed no expansion of LSKs (data not shown), suggesting that marrow adipocytes are negative regulators of HSC expansion and/or survival, and confirming the findings of two previous studies [ 42 , 43 ]. Similar proportions of LSK cells were found to be present whether undifferentiated MSCs or osteoblasts were used ( Figure 2 D).…”

Section: Resultssupporting

confidence: 88%

“…Conversely, however, when LSKs were seeded into cultures with adipocytes derived from MSCs, no growth was observed. This is supported by recent data suggesting that adipocytes have a negative influence on HSC growth [ 42 , 43 ], although a third report demonstrated the opposite effect [ 46 ]. Haematopoietic cells adhered to both MSCs and osteoblasts in co-culture, consistent with cobblestone area-forming cells (CAFC) and long-term culture-initiating cell (LTC-IC) assays that have been developed as surrogate in vitro assays in attempts to determine true HSC qualities.…”

Section: Discussionsupporting

confidence: 68%

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Engraftment Outcomes after HPC Co-Culture with Mesenchymal Stromal Cells and Osteoblasts

Cook

Doran

Kollár

et al. 2013

JCM

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Haematopoietic stem cell (HSC) transplantation is an established cell-based therapy for a number of haematological diseases. To enhance this therapy, there is considerable interest in expanding HSCs in artificial niches prior to transplantation. This study compared murine HSC expansion supported through co-culture on monolayers of either undifferentiated mesenchymal stromal cells (MSCs) or osteoblasts. Sorted Lineage− Sca-1+ c-kit+ (LSK) haematopoietic stem/progenitor cells (HPC) demonstrated proliferative capacity on both stromal monolayers with the greatest expansion of LSK shown in cultures supported by osteoblast monolayers. After transplantation, both types of bulk-expanded cultures were capable of engrafting and repopulating lethally irradiated primary and secondary murine recipients. LSKs co-cultured on MSCs showed comparable, but not superior, reconstitution ability to that of freshly isolated LSKs. Surprisingly, however, osteoblast co-cultured LSKs showed significantly poorer haematopoietic reconstitution compared to LSKs co-cultured on MSCs, likely due to a delay in short-term reconstitution. We demonstrated that stromal monolayers can be used to maintain, but not expand, functional HSCs without a need for additional haematopoietic growth factors. We also demonstrated that despite apparently superior in vitro performance, co-injection of bulk cultures of osteoblasts and LSKs in vivo was detrimental to recipient survival and should be avoided in translation to clinical practice.

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Section: Resultssupporting

confidence: 88%

Section: Discussionsupporting

confidence: 68%

Engraftment Outcomes after HPC Co-Culture with Mesenchymal Stromal Cells and Osteoblasts

Cook

Doran

Kollár

et al. 2013

JCM

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“…C-kit-cells -це плюрипотентна клітина з нормальним каріотипом, яка є безпосередньою похідною хоріальних структур плаценти та амніотичної рідини і не має поверхневого антигена [17]. Також виявлено, що C-kit (CD-117), як нащадок тотипотентних стовбурових клітин, може давати початок майже всім тканинам і органам [18].…”

Section: вступunclassified

“…Крім того, встановлено, що важливим у плацентарних тканинах є антиген CD-44. Він унікальний серед ядерних клітин ссавців тим, що мало стимулює галогенну реактивність [18][19][20].…”

Section: вступunclassified

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Features of immunohistochemical markers of stem CELLS CD-117, CD-44 in female placentas on the background of chronic stress

Bondarenko

2022

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Background. Placenta is a subject of interest to a wide range of scientists because it is rich in stem cells and their precursors. A stem cell is a cell that has the ability to self-repair and can differentiate into offspring (daughter cells) of one or more germ layers. In recent years, scientists have obtained new data of stem cells regenerative potential. However, only isolated publications about placental stem cells are available. Therefore, our studies about placental stem cells are important for discovery of structural and molecular mechanisms, their changes under the influence of chronic stress.Objective: to study the features of immunohistochemical markers of pluripotent stem cells and their morphological features.Materials and methods. We examined 80 women placentas with chronic stress in comparison with control using general histological and immunohistochemical methods in the following groups: group 1 – women placentas with physiological course of pregnancy in term 38–40 weeks, group 2 – women placentas with miscarriage, group 3 – women placentas with chronic stress due to internal irradiation (4.5 Bq/kg and more), group 4 – women placentas which had COVID-19 during pregnancy.Results. There was a significant increase of stem cell markers expression in the three study groups with a significant predominance in groups 3 and 4. It was also determined the different direction of their active factors.Conclusions. The general changes of all structures of the placental barrier are detected as a result of chronic stress due to various factors: micro detachment of the decidual membrane (significant increase in cases in the studied groups); malperfusion in the structures of the maternal placental barrier; in the placenta stem cells of the three study groups in comparison with the control were found stress markers. Thus, chronic stress due to various factors causes the same type of changes in placental structures, but they have different degrees of expression – with internal irradiation doses ≥ 4.8 Bq/kg, these changes are most expressive.

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Optimal Tissue Sources of Mesenchymal Stromal Cells for Clinical Applications

Heazlewood

Atkinson

2012

Mesenchymal Stem Cell Therapy

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Exploring the Human Term Placenta as a Novel Source for Stem Cells and their Application in the Clinic

Cited by 4 publications

References 94 publications

Engraftment Outcomes after HPC Co-Culture with Mesenchymal Stromal Cells and Osteoblasts

Engraftment Outcomes after HPC Co-Culture with Mesenchymal Stromal Cells and Osteoblasts

Features of immunohistochemical markers of stem CELLS CD-117, CD-44 in female placentas on the background of chronic stress

Optimal Tissue Sources of Mesenchymal Stromal Cells for Clinical Applications

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