Hematological and immune changes in Piaractus mesopotamicus in the sepsis induced by Aeromonas hydrophila

Claudiano, Gustavo da Silva; Yunis‐Aguinaga, Jefferson; Marinho-Neto, Fausto A.; Miranda, Renata Lima de; Martins, Isabela M.; Otani, Fabrízia Sayuri; Mundim, Antônio Vicente; Marzocchi-Machado, Cleni Mara; Moraes, Julieta Rodini Engrácia de; Moraes, Flávio Ruas de

doi:10.1016/j.fsi.2019.01.044

Cited by 42 publications

(23 citation statements)

References 34 publications

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“…Control group fish were injected by intraperitonal route with 0.5 mL of sterile 0.65% NaCl solution, while fish from the other groups were inoculated by the same route with 0.5 mL of A . hydrophila solution (1.78 x 10 9 CFU/mL) [11]. Specimens were maintained in fasting during the 24 h previous challenge.…”

Section: Methodsmentioning

confidence: 99%

“…hydrophila in fish [13]. Similarly, Claudiano et al [11] observed the firsts signs of Aeromonas infection, wich were petechiaes and suffusions, 9 hours post-inoculation of A . hydrophila in pacus, showing the importance of understanding aeromonosis.…”

Section: Introductionmentioning

confidence: 95%

“…The deleterious effects caused by Gram-negative bacteria, such as A . hydrophila , are consequences of endotoxins, triggering sepsis or endotoxemia; complex syndromes that are defined by the presence of a systemic inflammatory response (SIRS) [10,11]. The consequence of this exacerbated and uncontrolled inflammatory response is the appearance of the lesions in multiple organs and high mortality [12].…”

Section: Introductionmentioning

confidence: 99%

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Morphological, microbiological and ultrastructural aspects of sepsis by Aeromonas hydrophila in Piaractus mesopotamicus

et al. 2019

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Aeromonas bacteria can cause an infection characterized by septicemia and is one of the most common pathogens in tropical fish. This disease is responsible for high morbidity and mortality rates, causing considerable losses in aquaculture. Thus, the understanding of its pathophysiology is crucial to develop control strategies of this bacterial infection in farmed fish. This study aimed to characterize early pathological aspects of acute sepsis in pacu (Piaractus mesopotamicus) experimentally infected with Aeromonas hydrophila. A total of 160 juvenile pacus were inoculated intraperitoneally with A. hydrophila (1.78 x 109 CFU/mL) and at 0 (control), 1, 3, 6, and 9 hours post-inoculation (hpi), animals were anesthetized and samples were collected for microbiological, light microscopy and transmission electron microscopy (TEM) analyzes. The results showed the occurrence of hemodynamic alterations, such as hemorrhage and congestion, which were observed mainly after 6 and 9 hpi. It was possible to re-isolate Aeromonas at all sampling times except in control group. However, just after 9 hpi it was possible to find the bacteria in all fish and tissues. Light microscopy analyses revealed a degenerative process, necrosis and vascular damage mainly at 6 and 9 hpi. According to the ultrastructural examination, areas of cellular death were identified in all examined tissues, especially at 6 and 9 hpi. However, the most severe, related to necrosis, were observed after 6 and 9 hpi. The findings suggested that this bacterium spreads in the first hpi through the fish organs, mainly affecting spleen, liver and kidney, causing irreversible lesions at the molecular level.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Morphological, microbiological and ultrastructural aspects of sepsis by Aeromonas hydrophila in Piaractus mesopotamicus

et al. 2019

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“…However, the increase of its production in fish farms, frequently in intensive systems, facilitates the occurrence of disease outbreaks, leading to substantial economic losses. Among the infectious diseases commonly observed in farmed pacus, some of the most important are the ones caused by bacteria of the Aeromonas genus, which have been asso-ciated with septicemia-induced mortality outbreaks in this species in Brazil (Carriero et al, 2016;Claudiano et al, 2019;Marinho-Neto et al, 2019). Furthermore, the lack of knowledge regarding immune responses in P. mesopotamicus prevents the development of appropriate treatments and prophylactic strategies to decrease the frequency in which these outbreaks occur (Farias et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1β and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis

et al. 2020

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In the present study, the complete characterization of cDNA and genomic sequences of IL-1b and IL-8, as well as the expression profile of these genes in the South American fish pacu (Piaractus mesopotamicus) is provided. The full-length pmIL-1b cDNA was composed of 1208 nucleotides that would produce a precursor peptide with 273 amino acid residues. A putative caspase-1 cleavage site, similar to what is found in mammalian IL-1b, was identified producing a mature peptide with a theoretical molecular weight of 17.21 kDa. The pmIL-8 cDNA sequence consisted of 1019 nucleotides which encoded a 95-amino acid protein with a theoretical molecular weight of 10.43 kDa that showed all typical CXC chemokine features, including a 20-residue signal peptide and four conserved cysteine residues. Constitutive mRNA expression was detected for both genes in the liver, head kidney, gill, intestine, skin and spleen. After a bacterial challenge, up-regulation was detected for both pmIL-1b and pmIL-8 in the spleen and head kidney at 12 h post-infection. At 24 h post-infection there was a decrease in the expression of both genes, with pmIL-8 showing a significant down-regulation in the liver and head kidney when compared to the control groups.

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“…Biochemical variables (protein, albumin, magnesium and alanine aminotransferase) were determined using a semiautomatic analyser, and globulin and the albumin/globulin ratio was calculated (Brito et al, 2015;Claudiano et al, 2019). Blood glucose was determined according to Castro et al (2014).…”

Section: Blood and Serum Biochemical Assessmentsmentioning

confidence: 99%

Effect of multi‐species probiotic administration in Colossoma macropomum juvenile rearing: supplementation and bioremediation

et al. 2021

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Probiotics are defined as live microbial supplements that benefit the host (Fuller, 1989). In aquaculture, the definition is more comprehensive due to the fact that probiotics are applied by different means, such as feeding, injection or immersion in the culture water. Research on the use of probiotics in animal husbandry has increased with the increasing demands for environmentally sound agriculture/aquaculture practices and safer food (Liu & Han, 2004), especially when substituting antibiotics and growth promoters (Akhter et al., 2015).Fish gastrointestinal tract probiotics play an important role in improving zootechnical performance, ingredient digestibility, disease resistance and immune system responses (Mello et al., 2013;Silva et al., 2015). Furthermore, direct probiotic addition to fish farming water can result in several effects, with two main advantages, namely the ability to control water quality by bioremediation and pathogen biocontrol through antagonistic effects (Jahangiri & Esteban, 2018;Liu & Han, 2004).Studies on aquaculture-applied probiotics are of great interest in the aquaculture industry and market, and several options are

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Hematological and immune changes in Piaractus mesopotamicus in the sepsis induced by Aeromonas hydrophila

Cited by 42 publications

References 34 publications

Morphological, microbiological and ultrastructural aspects of sepsis by Aeromonas hydrophila in Piaractus mesopotamicus

Morphological, microbiological and ultrastructural aspects of sepsis by Aeromonas hydrophila in Piaractus mesopotamicus

Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1β and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis

Effect of multi‐species probiotic administration in Colossoma macropomum juvenile rearing: supplementation and bioremediation

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