Hematological and histopathological changes in silver catfishRhamdia quelen(Siluriformes) exposed to clomazone herbicide in the Madre River, Santa Catarina State, Southern Brazil

Abstract

This study evaluated the influence of the clomazone herbicide (2-[(2-chlorophenyl)methyl]-4,4-dimethyl-3-isoxazolidinone) contamination on the hematological parameters and histological changes in gills and liver of silver catfish (Rhamdia quelen) from Madre River, Santa Catarina State, Southern Brazil. Fish were collected between March 2010 and January 2012 at two different sites of the Madre River, one site receiving residual water (contaminated site) from rice culture (n=49) and another that do not receive residual water (reference site) (n=48). The herbicide clomazone analysis detected 3.40±1.70 μg/L in the contaminated site and 1.1±0.33 μg/L in the reference site. Fish from contaminated site showed increased (P<0.05) number of monocytes suggesting the possible defense response as a result of chronic exposure to clomazone. On the other hand, no difference was found in the hematocrit percentage, red blood cell count, total thrombocyte number, white blood cell count, lymphocytes, and neutrophils number. Fish from both sites showed histopathological changes in gills and liver, possibly caused by chronic exposure to contamination. The influence of herbicide sub doses on fish health is also discussed.

A histology-based study in apparently healthy wild freshwater stingrays Potamotrygon motoro

“…It is possible that despite the long evolution chain to reach the freshwater habitat, P. motoro still preserve the liver morphology of the marine elasmobranchs. Brum et al (2014) showed that silver catfish exposed to water from a polluted river (Madre) in Southern Brazil presented higher percentage of inflammatory cells in blood comparing to control group. In our study, 69.2 % of the fish samples presented large number of lymphocytes in the spleen and 23.1 % hyperemia comparing within the group (Fig.…”

The Use of Multiple Biomarkers to Assess the Health of Anuran Amphibians in the Brazilian Cerrado Savanna: An Ecotoxicological Approach

“…One of the most prominent alterations of the liver is the presence of lipidosis or steatosis, which are diagnosed by the accumulation of lipids in vacuoles of varying size found in the cytoplasm of the hepatocytes (Brum et al, 2014). Micro‐ and macrolipidosis has been found in the hepatocytes of catfish ( Rhamdia quelen ) exposed to the commercial formula of clomazone (Brum et al, 2014) and of carp ( Cyprinus carpio ) exposed to different concentrations of nanoparticles of TiO 2 (Federici et al, 2007; Linhua et al, 2009).…”

Influence of total length, sex and seasonal variations on hematological parameters in Cyprinus carpio (Linnaeus, 1758) (Pisces Cyprinidae), Lake Tonga (Algeria)

“…Other factors that can significantly affect hematological parameters in teleosts include reproductive cycle, diet, temperature, pH and photoperiod (Sandnes et al, 1988;Kavadias et al, 1996;Svoboda et al, 2001;Guijarro et al, 2003;Kavadias et al, 2003;Bayir, 2005), or stress, pollution, parasitism, size, seasonal variations (Clarks et al, 1979;Barham et al, 1980;Ranzani-Paiva et al, 2004;Camargo et al, 2005;Santos et al, 2009;Onyia et al, 2013;De Souza Neves et al, 2014;Brum et al, 2014;Figueiredo et al, 2014;Fallah et al, 2014).…”