Hematologic reference intervals in <scp>C</scp>ynomolgus (<i><scp>M</scp>acaca fascicularis</i>) monkeys

Bourgès‐Abella, Nathalie; Geffré, Anne; Moureaux, E.; Vincenti, M.; Braun, Jean‐Pierre; Trumel, Catherine

doi:10.1111/jmp.12077

Cited by 14 publications

(23 citation statements)

References 26 publications

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“…Although there are no obvious or otherwise documented phenotypic (i.e. physiological, biological, ecological) differences among these subpopulations of cynomolgus macaques on the island of Mauritius, given the results of our study it is entirely possible that observed phenotypic variability among Mauritian cynomolgus macaques [11, 42, 43] may accompany the genetic substructure. And while the substructure does not appear to be as stark or as significant as that between, for instance, Chinese- and Indian-origin rhesus macaques or subpopulations of cynomolgus macaques from Vietnam, Indonesia, and the Philippines, it has nevertheless aroused interest and investigation into these differences would be a meaningful avenue of future research.…”

Section: Resultsmentioning

confidence: 65%

“…The effects of genetic stratification or cryptic population substructure on studies, particularly where samples sizes are small, are significant [9]. In macaques, population differences have been identified with regards to behavior [10], physiology [11], susceptibility to infectious disease [12], and toxicology [13]. Therefore a priori genetic information on nonhuman primates used in research can aid in more informed selection of individuals for studies and better translational models.…”

Section: Introductionmentioning

confidence: 99%

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Genetic substructure in cynomolgus macaques (Macaca fascicularis) on the island of Mauritius

Ogawa

Vallender

2014

BMC Genomics

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BackgroundNonhuman primates are commonly used in biomedical research as animal models of human disease and behavior. Compared to common rodent models, nonhuman primates are genetically, physiologically, behaviorally and neurologically more similar to humans owing to more recent shared ancestry and therefore provide the advantage of greater translational validity in preclinical studies. The cynomolgus macaque (Macaca fascicularis) is one of the most commonly used nonhuman primates in academic and industry settings, yet population genetic research has revealed significant substructure throughout the species distribution that may confound studies. Cynomolgus monkeys introduced to Mauritius specifically have previously been thought to maintain the least genetic heterogeneity of all cynomolgus monkeys, although recent work, including work from our lab, suggests macaques from Mauritius too may harbor cryptic substructure.ResultsTo evaluate putative substructure in Mauritian cynomolgus macaques, we designed a panel of 96 single nucleotide polymorphisms based on preliminary findings from previous work to screen 246 of cynomolgus monkeys from two primary suppliers. Results from this study support substructure in Mauritian macaques and suggest a minimum of two populations and maybe three on Mauritius, with moderate admixture.ConclusionThese findings inform the natural history of these monkeys suggesting either a previously unrecognized physical or ecological barrier to gene flow on Mauritius and/or the breakdown of historic substructure resulting from the history of macaque introduction to the island. These findings are relevant to ongoing research using these models in part because of increased appreciation of segregating common variation with functional effects and may be used to better inform animal selection in preclinical research.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-748) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Genetic substructure in cynomolgus macaques (Macaca fascicularis) on the island of Mauritius

Ogawa

Vallender

2014

BMC Genomics

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“…Due to the housing conditions, we were able to assure that all monkeys were strictly fasted overnight, because food remains were retrieved after the last feeding schedule. Although it has been previously reported that fasting is not an absolute prerequisite in hematology [21], it could be clinically relevant in monkeys if the fasting duration exceeds 16 h [22]. Therefore, about 16-h fasting duration used in this study might be clinically relevant reference values for NHPs.…”

Section: Discussionmentioning

confidence: 90%

Reference values of hematological and biochemical parameters in young-adult cynomolgus monkey (Macaca fascicularis) and rhesus monkey (Macaca mulatta) anesthetized with ketamine hydrochloride

et al. 2019

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Nonhuman primate models are valuable in biomedical research. However, reference data for clinical pathology parameters in cynomolgus and rhesus monkeys are limited. In the present study, we established hematologic and biochemical reference intervals for healthy cynomolgus and rhesus monkeys anesthetized with ketamine hydrochloride. A total of 142 cynomolgus monkeys (28 males and 114 females) and 42 rhesus monkeys (22 males and 20 females) were selected and analyzed in order to examine reference intervals of 20 hematological and 16 biochemical parameters. The effects of sex were also investigated. Reference intervals for hematological and biochemical parameters were separately established by species (cynomolgus and rhesus) and sex (male and female). No sex-related differences were determined in erythrocyte-related parameters for cynomolgus and rhesus monkey housed in indoor laboratory conditions. Alkaline phosphatase and gamma glutamyltransferase were significantly lower in females than males in both cynomolgus and rhesus monkeys aged 48-96 months. The reference values for hematological and biochemical parameters established herein might provide valuable information for researchers using cynomolgus and rhesus monkeys in experimental conditions for biomedical studies.

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“…The Mauritian cynomolgus macaque population that descended from the small founder group consequently show a reduced genetic variability [13]. Consequently, differences in genetic composition [14] and phenotypic characteristics, such as body weight [15], sexual maturation rate [16], blood chemistry and haematology [17], and microbiome [18] compared with populations from within the species natural range of habitats in Asia have been reported. Furthermore, developments in the immunogenetic characterisation of Mauritian cynomolgus macaques have identified their value in the study of the role of host MHC genetics in immunogenicity and vaccine studies [19–27].…”

Section: Introductionmentioning

confidence: 99%

An aerosol challenge model of tuberculosis in Mauritian cynomolgus macaques

et al. 2017

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BackgroundNew interventions for tuberculosis are urgently needed. Non-human primate (NHP) models provide the most relevant pre-clinical models of human disease and play a critical role in vaccine development. Models utilising Asian cynomolgus macaque populations are well established but the restricted genetic diversity of the Mauritian cynomolgus macaques may be of added value.MethodsMauritian cynomolgus macaques were exposed to a range of doses of M. tuberculosis delivered by aerosol, and the outcome was assessed using clinical, imaging and pathology-based measures.ResultsAll macaques developed characteristic clinical signs and disease features of tuberculosis (TB). Disease burden and the ability to control disease were dependent on exposure dose. Mauritian cynomolgus macaques showed less variation in pulmonary disease burden and total gross pathology scores within exposure dose groups than either Indian rhesus macaques or Chinese cynomolgus macaquesConclusionsThe genetic homogeneity of Mauritian cynomolgus macaques makes them a potentially useful model of human tuberculosis.

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Hematologic reference intervals in Cynomolgus (Macaca fascicularis) monkeys

Abstract: These hematology reference intervals, established according to international recommendations, can be used in settings using similar animals and analyzers.

Cited by 14 publications

References 26 publications

Genetic substructure in cynomolgus macaques (Macaca fascicularis) on the island of Mauritius

Genetic substructure in cynomolgus macaques (Macaca fascicularis) on the island of Mauritius

Reference values of hematological and biochemical parameters in young-adult cynomolgus monkey (Macaca fascicularis) and rhesus monkey (Macaca mulatta) anesthetized with ketamine hydrochloride

An aerosol challenge model of tuberculosis in Mauritian cynomolgus macaques

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