Hematología y citoquímica de las células sanguíneas de Rhinella fernandezae (Anura: Bufonidae) en Espinal y Delta-Islas del río Paraná, Argentina

Abstract

Recibido 12-v-2010.Corregido 15-vIII-2010. Aceptado 17-IX-2010.Abstract: Hematology and blood cell cytochemistry of Rhinella fernandezae (Amphibia: Anura) from Espinal and Delta-Islands of Paraná River, Argentina. The description of amphibian hematology is scarce and most of these studies have been done in species from North America, Asia and Europe. With the purpose to obtain basic hematological information of Rhinella fernandezae, 23 blood samples from Santa Fe and Entre Ríos natural reserves were studied. Blood of each individual was extracted by cardiac puncture and hemograms were carried out. Morphological and cytochemical description of blood cells were analyzed in slides and were inspected for extra and intra cellular parasites. Five leucocytes types were observed, being lymphocytes the predominant ones followed by basophiles. Heterophils and eosinophils were positive to PAS, Sudan B and peroxidase. The erythrocytes and its precursors were negative for cytochemical reactions. Micronuclei and nuclear alterations frequencies were scarce. No significant differences (p>0.05) were observed between sexes neither in hemograms nor in blood cells morphology. Microfilarias were the only hemoparasites found with a relative low prevalence and infection intensity. The hematological characteristics studied were similar to those reported for other amphibians, suggesting that R. fernandezae individuals present optimal nutritional and immunological status. Rev. Biol. Trop. 59 (1): 17-28. Epub 2011 March 01.

Baseline values of immunologic parameters in the lizard <i>Salvator merianae</i> (<i>Teiidae</i>, Squamata)

“…Similar to what was seen by Troiano et al (1996), Silva (1998) andandRios et al (2003), this study did not reveal any differences among age classes in regard to other leukocyte types, while Stacy et al (2011) described age related changes in lymphocyte and heterophils percentages in Caretta caretta turtles. The lobularity index here observed was similar to the ones reported by Cabagna Zenklusen et al (2011) in Rhinella fernandezae and Salinas et al (2015) in Rhinella arenarum. With regard to NAb levels, our analysis of titres showed the highest values in the adults compared with juveniles and neonate http: //www.openveterinaryjournal.com A.P.…”

Effects of benzo[a]pyrene on the blood and liver of Physalaemus cuvieri and Leptodactylus fuscus (Anura: Leptodactylidae)

“…Despite the diversity of anurans, a great majority of the studies on their hematology are related to total cell count (erythrocytes and leukocytes) and morphometry of red blood cells (Cabagna Zenklusen et al, 2011), while detailed studies are lacking. The understanding of leukocyte responses of amphibians to diseases is limited (Campbell and Ellis, 2007), but is an important parameter for assessing organism health, demonstrating pollutant effects, and identifying biological changes caused by xenobiotics (Neta et al, 2015).…”

Comparative leukocyte morphometric analysis between endemic Anurans from Brazil and the invasive species Lithobates catesbeianus

“…Hematologic and biochemical evaluation of amphibians is of great clinical importance, and provides valuable diagnostic information and understanding of the pathogenesis of diseases, their progression, and response to therapy (SCHMID-SCHÖNBEIN et al, 1980;ALLENDER;FRY, 2008;DAVIS;DURSO, 2009;YOUNG et al, 2012). Nevertheless, there are few studies available on Neotropical amphibian hematology (CABAGNA-ZENKLUSEN et al, 2005;CABAGNA-ZENKLUSEN et al, 2011;LAJMANOVÍCH et al, 2012), and scarce studies on anuran white blood cell morphology (ARIKAN; ÇIÇEK, 2010;. Few morphometric studies of red blood cells (BARNI et al, 1992;ATATÜR et al, 1999;WOJTASZEK;ADAMOWICZ, 2003;GRENAT et al, 2009) and white blood cells (FOXON 1964;NANO et al, 1991;TOZETTI et al, 2014) are available.…”