Comparative leukocyte morphometric analysis between endemic Anurans from Brazil and the invasive species Lithobates catesbeianus

Silva, Stephanie Moira Rodrigues e; Ewbank, Ana Carolina; Strefezzi, Ricardo de Francisco; Alvarado, Gilbert; Sacristán, Carlos; Paula, Cátia Dejuste de; Catão‐Dias, José Luiz

doi:10.11606/issn.1678-4456.bjvras.2017.121887

Cited by 7 publications

(2 citation statements)

References 27 publications

(44 reference statements)

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“…Cell morphometrics has long been considered as an important indicator of cell plasticity ( Rodrigues et al 2017 ). Several physiological events cause morphological variations in size, volume, diameter, and relationships among structural components of red blood cells ( Allender and Fry 2008 ; Garcia-Neto et al 2020 ; Mahapatra et al 2012 ; Silva et al 2017 ). This may imply that subsequent adaptations that occur in blood cells involve alterations driven by critical physiological events ( Garcia-Neto et al 2020 ).…”

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confidence: 99%

Hematological parameters of a Neotropical wild frog population, with a phylogenetic perspective on blood cell composition in Anura

et al. 2021

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Hematological parameters can provide key information to an animal health status. However, this information is usually hard to obtain. Here, we described hematological parameters of Leptodactylus podicipinus in the Brazilian Pantanal. We measured red blood cell morphometrics, erythrogram, and leukogram. We also tested for phylogenetic signal in erythrogram and leukogram of 48 frog species from 15 families, testing if body size explains their variation. Lymphocytes were the most abundant leukocytes (> 60%) in Leptodactylus podicipinus, followed by neutrophils (∼10%). Given that L. podicipinus is an abundant and widely distributed species in central Brazil, knowing its hematological pattern can help stablish a baseline and improve its use as a bioindicator of environmental degradation. Mean corpuscular hemoglobin and value contributed more to the phylomorphospace of erythrogram, in which Leptodactylus spp. and Hypsiboas raniceps had lower values of these variables, while Bufotes viridis and Hyla arborea had high values. The phylogenetic signal was spread throughout the dimensions of leukogram phylomorphospace. The variables that most contributed to it were total leukocytes counts, lymphocytes and neutrophils. We also found a moderate phylogenetic signal for both the erythrogram and leukogram. Accordingly, body size accounted for a low proportion of variation in both the leukogram (4.7%) and erythrogram (0.57%). By combining phylogenetic comparative methods with hematological parameters, our results add a new perspective on the evolution of blood cell physiology in frogs.

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Hematological parameters of a Neotropical wild frog population, with a phylogenetic perspective on blood cell composition in Anura

et al. 2021

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“…Las poblaciones de anfibios no están al margen de esta problemática, puesto que desde hace tiempo se encuentran en franco retroceso a nivel mundial (Blaustein et al, 1994;Reaser, 1996;Houlahan et al, 2000;Stuart et al, 2004;Lips et al, 2005;Wake & Vredenburg, 2008;Simon et al, 2011;Carezzano et al, 2013ab). Es importante destacar que estos vertebrados son excelentes bioindicadores de calidad ambiental (Simon et al, 2011;Silva et al, 2017) y cumplen múltiples roles funcionales dentro de los ecosistemas acuáticos y terrestres (Blaustein & Wake, 1990;Stebbins & Cohen, 1995). ocular (DO), distancia interocular (DIO), distancia ojo-narina (DON), largo del antebrazo (LAB), largo del fémur (LF), largo de la tibia (LT) y largo del pie (LP) (Peltzer & Lajmanovich, 1999;Cortes Gómez et al, 2016).…”

Section: Introducción 52unclassified

Morfometría De Physalaemus Biligonigerus (Anura: Leptodactylidae) en Dos Ambientes Con Distinta Perturbación

Chiapero¹,

Mariscal²,

Panigo³

et al. 2019

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Argentina's agricultural frontiers have been expanding causing a fragmentation and simplification of ecosystems and, therefore, a decline of biodiversity, and conditions that affect amphibian populations. Morphometry is used as a support to explain biological processes and in anurans it allows to relate morphometric variations relation to different environmental contaminants. The objective of this study is to provide basic data about external morphometry and corporal conditions of adult individuals from both sexes of Physalaemus biligonigerus (Cope, 1861) that inhabit a highly disturbed environment. It's important to point out that amphibians' changes in size and corporal conditions has been proposed as a reference to estimate environmental deterioration. The specimens analyzed were in two different environments with different antropic perturbation (agroecosystem and forest). Their corporal mass was registered (MC) as well as nine morphometric variables: snout to vent lenght (LHC), mouth width (AB), eye width (DO), inter-orbital distance (DIO), eye-nose distance (DON), forearm length (LAB), femur length (LF), tibia length (LT) and hindlimb toe length (LP). A corporal condition factor has been estimated 3 throught the ecuation K=[MC/LHC ]*100.000 to evaluate the general condition of the individuals. Compared to individuals coming from the forest the agroecosystem's specimens showed significant lower values in seven morphometric variables in females (LHC, DIO, DON, LB, LF, LT y LP) and five in males (LHC, DIO, DON, LT y LP). Also, it was observed that in both sexes the corporal condition factor was lower in the individuals coming from the agroecosystem. It concludes that the differences found between the analyzed populations might be related to the level of antropic perturbation.

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Artificial light at night and captivity induces differential effects on leukocyte profile, body condition, and erythrocyte size of a diurnal toad

2018

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Light pollution or artificial lighting at night (ALAN) is an emerging threat to biodiversity that can disrupt physiological processes and behaviors. Because ALAN stressful effects are little studied in diurnal amphibian species, we investigated if chronic ALAN exposure affects the leukocyte profile, body condition, and blood cell sizes of a diurnal toad. We hand‐captured male toads of Melanophryniscus rubriventris in Angosto de Jaire (Jujuy, Argentina). We prepared blood smears from three groups of toads: “field” (toads processed in the field immediately after capture), “natural light” (toads kept in the laboratory under captivity with natural photoperiod), and “constant light” (toads kept in the laboratory under captivity with constant photoperiod/ALAN). We significantly observed higher neutrophil proportions and neutrophils to lymphocytes ratio in toads under constant light treatment. In addition, we observed significantly better body condition and higher erythrocyte size in field toads compared with captive toads. In summary, ALAN can trigger a leukocyte response to stress in males of the diurnal toad M. rubriventris. In addition, captivity can affect the body condition and erythrocyte size of these toads.

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Comparative leukocyte morphometric analysis between endemic Anurans from Brazil and the invasive species Lithobates catesbeianus

Cited by 7 publications

References 27 publications

Hematological parameters of a Neotropical wild frog population, with a phylogenetic perspective on blood cell composition in Anura

Hematological parameters of a Neotropical wild frog population, with a phylogenetic perspective on blood cell composition in Anura

Morfometría De Physalaemus Biligonigerus (Anura: Leptodactylidae) en Dos Ambientes Con Distinta Perturbación

Artificial light at night and captivity induces differential effects on leukocyte profile, body condition, and erythrocyte size of a diurnal toad

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