Hemagglutination by Pasteurellaceae isolated from rodents

Boot, R.; Thuis, H. C. W.; Teppema, J. S.

doi:10.1016/s0934-8840(11)80404-4

Cited by 17 publications

(7 citation statements)

References 27 publications

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“…However, Boot et al [3] showed thin fimbriae by using negative staining and electron microscopy. Using transmission electron microscopy, we recently revealed the outer structure of the cell wall of P. pneumotropica [11].…”

Section: Discussionmentioning

confidence: 99%

Pathogenicity of Pasteurella pneumotropica in Immunodeficient NOD/ShiJic-scid/Jcl and Immunocompetent Crlj:CD1 (ICR) Mice

et al. 2011

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Abstract:Pasteurella pneumotropica is an opportunistic pathogen in rodents. Natural infection in immunodeficient animals suggests that immunodeficiency is a major factor in P. pneumotropica pathogenesis. To understand this process, we performed clinical, pathological and bacteriological studies of immunodeficient NOD/ShiJic-scid/Jcl and immunocompetent Crlj:CD1 (ICR) mice experimentally infected with P. pneumotropica ATCC 35149. From 14 days postinoculation, some of P. pneumotropica-infected NOD/ShiJic-scid/Jcl mice developed clinical signs of weight loss. Three of 10 P. pneumotropica-infected NOD/ShiJic-scid/Jcl mice developed clinical signs of depression, ruffled coat, and weight loss and died at 27, 34, and 59 days postinoculation. At 35 days postinoculation, almost all P. pneumotropica-infected NOD/ShiJic-scid/Jcl mice had lung abscesses. The bacteria were isolated from the upper and lower respiratory tracts, including the lungs, and blood. In contrast, P. pneumotropicainfected ICR mice exhibited no clinical signs or lesions. The bacteria were isolated from the upper, but not the lower respiratory tracts. We developed an animal model for understanding host interactions with P. pneumotropica.

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Section: Discussionmentioning

confidence: 99%

Pathogenicity of Pasteurella pneumotropica in Immunodeficient NOD/ShiJic-scid/Jcl and Immunocompetent Crlj:CD1 (ICR) Mice

et al. 2011

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“…The phenotypic characteristics related to the virulence of P. pneumotropica are hemagglutination and hemolysis [11-13]. Two recently named exoproteins, PnxIA and PnxIIA, both of which have C-terminal primary structures similar to the repeat in structural toxin (RTX) toxins, have been identified and characterized as hemolysin-like proteins in P. pneumotropica [13].…”

Section: Introductionmentioning

confidence: 99%

Molecular and virulence characteristics of an outer membrane-associated RTX exoprotein in Pasteurella pneumotropica

et al. 2011

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BackgroundPasteurella pneumotropica is a ubiquitous bacterium that is frequently isolated from laboratory rodents and causes various clinical symptoms in immunodeficient animals. Currently two RTX toxins, PnxIA and PnxIIA, which are similar to hemolysin-like high-molecular-weight exoproteins are known in this species. In this study, we identified and analyzed a further RTX toxin named PnxIIIA and the corresponding type I secretion system.ResultsThe RTX exoprotein, PnxIIIA, contains only a few copies of the RTX repeat-like sequence and 3 large repeat sequences that are partially similar to the outer membrane protein found in several prokaryotes. Recombinant PnxIIIA protein (rPnxIIIA) was cytotoxic toward J774A.1 mouse macrophage cells, whereas cytotoxicity was attenuated by the addition of anti-CD11a monoclonal antibody. rPnxIIIA could bind to extracellular matrices (ECMs) and cause hemagglutination of sheep erythrocytes. Binding was dependent on the 3 large repeat sequences in PnxIIIA. Protein interaction analyses indicated that PnxIIIA is mainly localized in the outer membrane of P. pneumotropica ATCC 35149 in a self-assembled oligomeric form. PnxIIIA is less cytotoxic to J774A.1 cells than PnxIA and PnxIIA.ConclusionsThe results implicate that PnxIIIA is located on the cell surface and participates in adhesion to ECMs and enhanced hemagglutination in the rodent pathogen P. pneumotropica.

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“…For the microbiological control of laboratory rodents, P. pneumotropica is one of the pathogens that must be prevented in rodent colonies. In P. pneumotropica infections, clinical diseases have generally presented with skin lesions, ophthalmitis, conjunctivitis, and otitis media (2); furthermore, clinical diseases have also been known to lead to fatal pneumonia in immunodeficient animals (15,24,26).Although details of the virulence factors of P. pneumotropica are still unavailable, predicted virulence associations have been reported (4,15,16,26). Of these, hemolysis is one of the phenotypic characteristics widely distributed in pathogenic bacteria.…”

mentioning

confidence: 99%

“…Although details of the virulence factors of P. pneumotropica are still unavailable, predicted virulence associations have been reported (4,15,16,26). Of these, hemolysis is one of the phenotypic characteristics widely distributed in pathogenic bacteria.…”

mentioning

confidence: 99%

Identification and Characterization of Hemolysin-Like Proteins Similar to RTX Toxin in Pasteurella pneumotropica

Sasaki

Kawamoto

Tanaka³

et al. 2009

J Bacteriol

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Pasteurella pneumotropica is an opportunistic pathogen that causes lethal pneumonia in immunodeficient rodents. The virulence factors of this bacterium remain unknown. In this study, we identified the genes encoding two RTX toxins, designated as pnxI and pnxII, from the genomic DNA of P. pneumotropica ATCC 35149 and characterized with respect to hemolysis. The pnxI operon was organized according to the manner in which the genes encoded the structural RTX toxin (pnxIA), the type I secretion systems (pnxIB and pnxID), and the unknown orf. The pnxII gene was involved only with the pnxIIA that coded for a structural RTX toxin. Both the structural RTX toxins of deduced PnxIA and PnxIIA were involved in seven of the RTX repeat and repeat-like sequences. By quantitative PCR analysis of the structural RTX toxin-encoding genes in P. pneumotropica ATCC 35149, the gene expression of pnxIA was found to have increased from the early log phase, while that of pnxIIA increased from the late log to the early stationary phase. As expressed in Escherichia coli, both the recombinant proteins of PnxIA and PnxIIA showed weak hemolytic activity in both sheep and murine erythrocytes. On the basis of the results of the Southern blotting analysis, the pnxIA gene was detected in 82% of the isolates, while the pnxIIA gene was detected in 39%. These results indicate that the products of both pnxIA and pnxIIA were putative associations of virulence factors in the rodent pathogen P. pneumotropica.Pasteurella pneumotropica is a gram-negative, rod-shaped bacterium that is frequently isolated from the upper respiratory tracts, digestive tracts, and vaginas of rodents (2,26). This bacterium is the major cause of pasteurellosis in immunodeficient and immunosuppressed animals, while certain effects on health are not observed in immunocompetent animals (2, 26). For the microbiological control of laboratory rodents, P. pneumotropica is one of the pathogens that must be prevented in rodent colonies. In P. pneumotropica infections, clinical diseases have generally presented with skin lesions, ophthalmitis, conjunctivitis, and otitis media (2); furthermore, clinical diseases have also been known to lead to fatal pneumonia in immunodeficient animals (15,24,26).Although details of the virulence factors of P. pneumotropica are still unavailable, predicted virulence associations have been reported (4,15,16,26). Of these, hemolysis is one of the phenotypic characteristics widely distributed in pathogenic bacteria. In particular, many pathogens that belong to the family Pasteurellaceae possess one of the pore-forming protein toxins occurring concurrently with hemolysis-a toxin determined to be a repeat in the structural toxin (RTX toxin) (42). RTX toxins are recognized as members of the type I exoprotein secretion system and were first characterized as hemolysins and leukotoxins produced by Actinobacillus pleuropneumoniae, Aggregatibacter (Actinobacillus) actinomycetemcomitans, Bordetella pertussis, enterohemorrhagic Escherichia coli, and Mannheimia haemolyti...

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Hemagglutination by Pasteurellaceae isolated from rodents

Cited by 17 publications

References 27 publications

Pathogenicity of Pasteurella pneumotropica in Immunodeficient NOD/ShiJic-scid/Jcl and Immunocompetent Crlj:CD1 (ICR) Mice

Pathogenicity of Pasteurella pneumotropica in Immunodeficient NOD/ShiJic-scid/Jcl and Immunocompetent Crlj:CD1 (ICR) Mice

Molecular and virulence characteristics of an outer membrane-associated RTX exoprotein in Pasteurella pneumotropica

Identification and Characterization of Hemolysin-Like Proteins Similar to RTX Toxin in Pasteurella pneumotropica

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