Hemagglutination Activity ofLactobacillus acidophilusGroup Lactic Acid Bacteria

Abstract

The cells of 28 strains of the Lactobacillus acidophilus group were evaluated for hemagglutination (HA) activity. The activity was found in the surface layer (SL) protein fraction extracted by 2 M guanidine hydrochloride. The most SL proteins from the A group strains (L. acidophilus (A1), L. crispatus (A2), L. amylovorus (A3), and L. gallinarum (A4)) showed HA activity, but the proteins from the B group strains (L. gasseri (B1) and L. johnsonii (B2)) showed no activity. The SL proteins from the A group strains were composed in common of a main component having molecular mass of about 40-45 kDa on SDS-PAGE. The SL proteins from JCM 1034 strain that showed the highest HA activity was fractionated by CM-Toyopearl ion-exchange chromatography. The highest HA activity was detected in the major protein of 41 kDa. This protein was purified and shown to be composed of about 50% of hydrophobic amino acids. The HA activity of the protein (1034 lectin) was specifically inhibited by fetuin and bovine lactoferrin at the concentrations of 80 and 160 micrograms/ml, respectively. The removal of N-acetylneuraminic acid from fetuin significantly decreased the inhibitory activity.

A Mucus Adhesion Promoting Protein, MapA, Mediates the Adhesion ofLactobacillus reuterito Caco-2 Human Intestinal Epithelial Cells

“…Several LAB are known to adhere to the GI tract through a lectin-like function, by the affinity of a bacterial extracellular molecule and mucus or epithelial cells. 5,[30][31][32] Previously, the influence of proteinase treatment on the adhesion of LAB was investigated, and several strains of LAB were found probably to possess a proteinaceous adhesin(s) on their cell surface. 7) In this study, the adhesion factors of intestinal cells are strongly indicated also to be proteinous molecules in MapA mediating adhesion.…”

A New Screening Method for the Selection ofLactobacillus acidophilusGroup Lactic Acid Bacteria with High Adhesion to Human Colonic Mucosa

“…We have already reported that the 1034 lectin (purified) had high HA activity and could recognize a trisaccharide portion (NeuAc:x2-.6GaI/Jl-.4GlcNAc-), common in the (8). and YN266 (C) SLP.…”

“…The binding to the Carnoy's-fixed surface mucous layer of human normal colon tissues with B-I034 SLP (whole) and B-I034 lectin (purified) prepared from L. acidophilus JCM I 034 in our previous paper 8 ) was examined by histochemical staining. Figure 2C shows the mucous layer of Carnoy's-fixed human colon seen by the HID (for sulfated mucopolysaccharide)-AB (for other acidic mucopolysaccharide) double staining method.…”

“…8 ) Although the HA assay has been used in our previous experiments for the first screening stage of lectin-like components on lactic acid bacterial surface, little is known about the relationship between the HA activity and the actual adhesion to the human colonic mucous layer. Podolsky has reported 9 ) the chemical structure of 21 kinds of acidic and neutral O-glycosidic sugar chains (mucin type) combining to human colonic mucin.…”

Presence of Intestinal Lectin-binding Glycoproteins on the Cell Surface ofLactobacillus acidophilus

“…Also, it has been reported that lectin-like activity of L. acidophilus group LAB was specific for strains. 27 ) To confirm this speculation, efforts will be directed to examine adhesion of L. acidophilus group LAB to epithelial cells of intestine with many bacterial strains.…”