A New Screening Method for the Selection of<i>Lactobacillus acidophilus</i>Group Lactic Acid Bacteria with High Adhesion to Human Colonic Mucosa

Takahashi, Noritoshi; Saito, Tadao; Ohwada, Shyuichi; Ota, Hiroyoshi; Hashiba, Honoh; Itoh, Takatoshi

doi:10.1271/bbb.60.1434

Cited by 13 publications

(9 citation statements)

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“…In a number of studies, the loss of the S-layer protein from the bacterial surface by chemical means (Kos et al 2003; Garrote et al 2004; Frece et al 2005; Chen et al 2007; Jakava-Viljanen and Palva 2007; Tallon et al 2007) or the covering of the layer by other molecules during prolonged cultivation (Schneitz et al 1993) has been shown to decrease adhesion to different targets, but the role of the S-layer protein in adherence in these studies has not been directly demonstrated. The haemagglutinating activity of L. acidophilus JCM 1034 and the mucin binding activities of related strains were shown to be linked to their S-layer proteins, although the involvement of other guanidine hydrochloride-extractable components of the cell wall in this lectin-like activity could not be excluded, and/or the effect of aggregation of the S-layer proteins possibly causing unspecific effects could not be completely ruled out (Yamada et al 1994; Takahashi et al 1996). Likewise, in the study of Golowczyc et al (2009), where the carbohydrate-dependent co-aggregation of L. kefir with yeast or red blood cells was suggested to be S-layer-mediated, conclusions were drawn from the effects of LiCl and SDS treatments of L. kefir cells, and the solubility of the S-layer proteins in the LiCl extracts of L. kefir used in the aggregation assays was not demonstrated.…”

Section: Functions Of Lactobacillus S-layer Proteinsmentioning

confidence: 99%

Lactobacillus surface layer proteins: structure, function and applications

Hynönen

Palva

2013

Appl Microbiol Biotechnol

214

196

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Bacterial surface (S) layers are the outermost proteinaceous cell envelope structures found on members of nearly all taxonomic groups of bacteria and Archaea. They are composed of numerous identical subunits forming a symmetric, porous, lattice-like layer that completely covers the cell surface. The subunits are held together and attached to cell wall carbohydrates by non-covalent interactions, and they spontaneously reassemble in vitro by an entropy-driven process. Due to the low amino acid sequence similarity among S-layer proteins in general, verification of the presence of an S-layer on the bacterial cell surface usually requires electron microscopy. In lactobacilli, S-layer proteins have been detected on many but not all species. Lactobacillus S-layer proteins differ from those of other bacteria in their smaller size and high predicted pI. The positive charge in Lactobacillus S-layer proteins is concentrated in the more conserved cell wall binding domain, which can be either N- or C-terminal depending on the species. The more variable domain is responsible for the self-assembly of the monomers to a periodic structure. The biological functions of Lactobacillus S-layer proteins are poorly understood, but in some species S-layer proteins mediate bacterial adherence to host cells or extracellular matrix proteins or have protective or enzymatic functions. Lactobacillus S-layer proteins show potential for use as antigen carriers in live oral vaccine design because of their adhesive and immunomodulatory properties and the general non-pathogenicity of the species.

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Section: Functions Of Lactobacillus S-layer Proteinsmentioning

confidence: 99%

Lactobacillus surface layer proteins: structure, function and applications

Hynönen

Palva

2013

Appl Microbiol Biotechnol

214

196

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“…The binding of lectin-like protein(s) in SLP to the colonic mucous layer was confirmed by histochemical staining using human colon tissues fixed with Carnoy's fixative (Takahashi et al 1996). The binding of lectin-like protein(s) in SLP to the colonic mucous layer was confirmed by histochemical staining using human colon tissues fixed with Carnoy's fixative (Takahashi et al 1996).…”

Section: ) Second Stepmentioning

confidence: 72%

“…Surface layer proteins were prepared from cells of L. acidophilus group LAB bacterial selected by a mass screening in the above-described first step. The binding of lectin-like protein(s) in SLP to the colonic mucous layer was confirmed by histochemical staining using human colon tissues fixed with Carnoy's fixative (Takahashi et al 1996). Saito et al (2000) proposed a new method for estimation of the sugar portion on the human mucosal layer by using selected bacteria with a commercial biotinylated carbohydrate probe that has representative partial structures of the sugar chains constituting HCM.…”

Section: ) Second Stepmentioning

confidence: 98%

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Selection of useful probiotic lactic acid bacteria from the Lactobacillus acidophilus group and their applications to functional foods

Saito

2004

Animal Science Journal

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In the present review, a new mass screening system for selecting probiotic strains from Lactobacillus ( L) acidophilus group lactic acid bacteria (LAB) with strong adhesion to the human intestinal tract is described. Characteristics of antimicrobial peptides (bacteriocin), lactose-hydrolyzing enzymes and immunostimulative oligo DNA motifs in L. gasseri strains are also described. Finally, the use of L. acidophilus LAB, selected by our screening method, that have strong adhesion to the human colonic mucosa in functional yogurt products is described. Adhesiveness to the human intestine is one of the most important characteristics of probiotic LAB. A new screening system that involves a combination of three methods is proposed: rat colonic mucin (RCM)-micro plate assay, Carnoy's histochemical staining method and carbohydrate probe binding assay. By using an RCM-coated poly-vinylidene-diflouride membrane that mimics the human colonic mucous layer, a new lectin was isolated and its structure was clarified by gene cloning. Furthermore, the structures and functions of a new cyclic bacteriocin (gassericin A), new lactose-hydrolyzing enzymes, and new immunostimulating oligo DNA motifs from Lactobacillus gasseri (B 1 subgroup) were clarified. A new functional yogurt 'Fit down' is proposed, that is fermented by an adhesive strain of L. acidophilus LA67 selected by our screening and contains antihypertensive peptides derived from whey proteins by protease digestion. In the future, superior functional foods containing more effective probiotic LAB are expected to be developed by the use of the proposed mass screening system.

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“…In order to colonize the intestine, the bacteria must interact with the mucosal surface. Lactobacillus strains have been found to adhere to cultured human colonic epithelial cells (Greene and Klaenhammer 1994 ;Adlerberth et al 1996 ;Sarem et al 1996) and to bind to mucin covering the mucosal surface of the human colon (Takahashi et al 1996), or the pig intestine (Henriksson and Conway 1996).…”

Section: Introductionmentioning

confidence: 99%