Hemadsorption in Monkey Kidney Cell Cultures of Mycoplasma (PPLO) Recovered from Rats.

Berg, Robert B.; Frothingham, Thomas E.

doi:10.3181/00379727-108-27013

Cited by 16 publications

(5 citation statements)

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“…Adsorption could not be demonstrated in strain EA. M. pulmonis strain Ti and M. gallisepticum strongly adsorbed both sheep and guinea pig cells; this supports the findings of other workers (1,24,25). The cat strains CO, Bi, and B2 adsorbed sheep cells only.…”

Section: Methodssupporting

confidence: 90%

Hemolysin and Peroxide Activity ofMycoplasmaSpecies

1968

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Various methods for the detection of hemolysin production by Mycoplasma species were compared. Inoculation of blood-agar by the push-block method and by use of concentrated mycoplasma cell suspensions was compared with the agaroverlay technique. The preferred method was direct surface inoculation of concentrated suspensions onto the blood-agar. Among the conditions tested, refrigeration of 48-hr cultures gave the best results. A wide variety of mycoplasma species were tested for hemolytic activity towards rabbit, sheep, guinea pig, duck, and chicken bloods. Guinea pig erythrocytes were found to be the most susceptible to lysis by mycoplasma, and rabbit erythrocytes were found to be the least susceptible. A sensitive technique for the detection of peroxide production by mycoplasma strains, employing agar containing benzidine and sheep blood, was used. With this method, peroxide production could be correlated with hemolysis on blood-agar. Peroxidase and catalase inhibited both the benzidine reaction and hemolysis. It was concluded that the major hemolysin of the Mycoplasma species examined is a peroxide. 157 GC2 M. fermentans G M. salivarium 156 04 and 013A M. orale types 1 and 2 Strain Washington Strain 4330 M. arthritidis 14152 (Campo) 158 (Campo) PB PN L4P (Preston) L4S 14124 El M. pulmonis Ti M. neurolyticum TA M. gallinarum M. gallisepticum Bit M. mycoides var. capri Strain tumor M. bovigenitalium 14173 Strain goat Strain bovine Cell culture strain Hela B Cell culture strain FL Cat strains Human genital tract Human genital tract Rat abscess Rat abscess Rat arthritis Rat arthritis Rat cancer tissue Inflammed rat eye Benign rat tumor Mouse brain Respiratory tract of fowl Turkey brain Goat Rat lymphosarcoma Bovine genital tract Cell cultures Cell cultures Cat conjunctiva Cat saliva Cat saliva

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Section: Methodssupporting

confidence: 90%

Hemolysin and Peroxide Activity ofMycoplasmaSpecies

1968

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“…Thus, the PG34 strain of the latter haemadsorbed, but the 'Negroni' strain of the same serotype did not. It is interesting that some strains of M. pulmonis haemadsorb, since Berg & Frothingham (1961) reported that tissue culture cells infected with this organism haemadsorbed. Whether colonies of a non-haemadsorbing mycoplasma strain could acquire by continuous passage the ability to haemadsorb or, conversely, whether those of a haemadsorbing mycoplasma could lose this ability is unknown.…”

Section: Discussionmentioning

confidence: 99%

Haemadsorption and Haemagglutination by Mycoplasmas

Manchee¹,

Taylor‐Robinson²

1968

Journal of General Microbiology

165

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SUMMARYFactors concerned in demonstrating haemadsorption and haemagglutination and their occurrence among different mycoplasmas were investigated. Haemadsorption occurred best to colonies which had recently developed on agar at pH 6.5. Mycoplasmas isolated from various bird and animal sources, e.g. Mycoplasma gallisepticum, M. agalactiae, M. bovigenitalium and M . pulmonis, haemadsorbed with erythrocytes from a wide range of species. However, not all strains within a serotype haemadsorbed. Thus, the 'Negroni' strain of M . pulmonis did not. Haemadsorption could be inhibited by crowding of colonies on agar and by the addition of specific antiserum to the colonies. Generally, antiserum titres obtained by haemadsorption inhibition were low in comparison with those obtained by metabolic inhibition, and haemadsorption inhibition was not useful as a routine serological technique.The development of the haemagglutinin of Mycoplasma gallisepticum in liquid medium was studied in detail; a change in the pH value of the medium could be used as an index of its development and it was intimately associated with the organism. The centrifuged deposits of other mycoplasmas, from birds, cattle, goats, man, rodents and pigs, and which were grown in liquid medium also haemagglutinated, but generally to low titre. Haemagglutination occurred best in U-shaped cups at 37' and at pH 6.5-7-0 and could be inhibited by specific antiserum. There was lack of correlation between haemadsorption and haemagglutination; both these phenomena were exhibited by some mycoplasmas, others haemadsorbed only, and still others haemagglutinated only.

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“…Probably one of the most confusing properties is the capacity to produce CPE in cells. Other common properties are their filterability through 0.22-nm Millipore membrane filters (117), growth inhibition by specific antiserum (43), sensitivity to chloroform and ether (74), hemagglutination (2), hemadsorption (19,40), resistance to certain antibiotics, morphology by electron microscopy, and ability to induce chromosomal aberrations and morphological changes in infected cells.…”

Section: Cases Of Mistaken Identitymentioning

confidence: 99%

Mycoplasmas and cell cultures.

Stanbridge¹

1971

Bacteriological Reviews

118

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Such terms are now in disfavor and the correct generic name, Mycoplasma, should be used. Recently the mycoplasmas have been classified into two separate families, the sterol-requiring Mycoplasmataceae and the sterol-nonrequiring Acholeplasmataceae (44). To the present time, over 30 named species of mycoplasmas have been included within the two families. In addition there are several unidentifiable isolates (L. Hayflick, personal communication). The above classification refers to classic (large-colony) mycoplasmas. It does not include T-strain mycoplasmas (so called, because they form tiny colonies approximately one-fourth the size of classic mycoplasma colonies) on agar. T-strain mycoplasmas were first isolated by Shepard from human urino-genital tracts (170). They have now been isolated from various animals. The T-strain group, as a whole, is unclassified at the present time. A unique requirement for urea (171), in addition to sterol requirement, for growth of these mycoplasmas has prompted the suggestion that they should be included as a separate genus in the order Mycoplasmatales (156a). MORPHOLOGY OF MYCOPLASMAS The mycoplasma organism is distinctive for its small size of 130 to 300 nm and for the lack of a cell wall, the limiting boundary being a plasma 206

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Hemadsorption in Monkey Kidney Cell Cultures of Mycoplasma (PPLO) Recovered from Rats.

Cited by 16 publications

References 1 publication

Hemolysin and Peroxide Activity ofMycoplasmaSpecies

Hemolysin and Peroxide Activity ofMycoplasmaSpecies

Haemadsorption and Haemagglutination by Mycoplasmas

Mycoplasmas and cell cultures.

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