HEMA reduces cell proliferation and induces apoptosis in vitro

Samuelsen, Jan Tore; Holme, Jørn A.; Becher, Rune; Karlsson, Stig; Morisbak, Else; Dahl, Jon E.

doi:10.1016/j.dental.2007.08.006

Cited by 50 publications

(45 citation statements)

References 32 publications

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“…In the comparison with the cytological samples of metal ceramic crowns of the current study there were percentage of apoptotic cells caused by composite filling and this is in agreement with Samuelsen et al 28 and Schweikl et al 29 . This effect may be due to an increase the duration of composite resin fillings (more than 1week) that induced an elevation of the monomer transformation rate based on the chemical nature of these materials and also there was an increase in the dimethacrylates cytotoxicity after more than (72hr) where the treated cell cultures could not able to modify their form that were destroyed cells 29 .…”

Section: Discussionsupporting

confidence: 79%

The influence of some iatrogenic factors on a cytomorphometric analysis of gingival cells and periodontal status among diabetic patients

Abdaly¹,

Almalki²,

Saleh³

2017

Int J Recent Sci Res

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Section: Discussionsupporting

confidence: 79%

The influence of some iatrogenic factors on a cytomorphometric analysis of gingival cells and periodontal status among diabetic patients

Abdaly¹,

Almalki²,

Saleh³

2017

Int J Recent Sci Res

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“…They include tests for estimating the amount of ribonucleic acids and the damage to their chains [12], assessing the glutathione level in cells [13], assessing the expression of heat shock proteins [14], or studies evaluating the severity of apoptotic action [15]. Each of these methods has advantages and disadvantages, but provide extra information that cannot be obtained by other methods.…”

Section: Introductionmentioning

confidence: 99%

Uncompleted polymerization and cytotoxicity of dental restorative materials as potential health risk factors

Małkiewicz

Wychowański

Olkowska-Truchanowicz

et al. 2017

Ann Agric Environ Med.

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Małkiewicz K, Wychowański P, Olkowska-Truchanowicz J, Tykarska M, Czerwiński M, Wilczko M, Owoc A. Uncompleted polymerization and cytotoxicity of dental restorative materials as potential health risk factors. Ann Agric Environ Med. 2017; 24(4): 618-623. doi: 10.5604/12321966.1235159 Abstract Introduction. Composite materials used in dentistry indicate adverse biological effects in laboratory conditions. One reason for this activity is incomplete conversion of their polymer matrix, favoring chemical instability and release of biologically harmful components to the external environment. Aim. The aim of the study was to assess the degree of conversion of restorative materials commonly available on the European market and to examine the cytotoxic effects of their eluates in vitro. Material and methods. Using the Fournier transform infrared spectroscopy (FTIR) technique of analysis, the degree of polymer matrix conversion of 6 restorative materials was examined: Gradia Direct, Arkon, Filtek Z550, Herculite XRV, Tetric Evo Ceram, Charisma, polymerized with LED light. In order to assess the cytotoxicity of eluates of the studied materials obtained after 1 hour, 24 hours and 7 days, the MTT assay was used in cultured 3T3 cells. The results were statistically analyzed at significance level of p=0.05. Results. The conversion degree of the assessed polymers ranged from 31.56% for Tetric Evo Ceram to 75.84% for Arcon. The strongest (p=0.05) cytotoxic effect was demonstrated after 7-day observation of Tetric Evo Ceram eluates, reducing the metabolic activity of cells down to 56%. A positive correlation (r(x, y)=0.62) between the degree of conversion of composite materials and cytotoxic effects of their eluates on cell cultures was confirmed. Conclusions.1. Restorative dental materials are chemically unstable in the conditions of the present study. 2. Polymer-based restorative dental materials available on the European market demonstrate cytotoxic properties constituting a potential threat to the patients' health.

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“…If released at low concentrations for a prolonged period of time, HEMA could reduce the rate of cellular proliferation and result in apoptosis, possibly as a response to DNA damage (Samuelsen et al, 2008).…”

Section: Dental Polymersmentioning

confidence: 99%

“…Several types of cells can be used for cytotoxicity tests such as cell lines -human and mouse fibroblasts, lymphocytes, queratinocytes, mouse odontoblast-like cells, mouse macrophages, rat submandibular salivary gland acinar cells - (Bakoupoulou et al, 2007;Franz et al, 2009;Kostoryz et al, 2003;Lin et al, 2007;Roll et al, 2004;Samuelsen et al, 2008), and primary cell types -human lymphocytes, polymorphonuclear leukocytes, and mixed leukocytes, mouse blastocysts, mouse macrophages, and mouse embryo cells - (Becher et al, 2006;Huang & Chang, 2002;Jonhson et al, 1985;Libonati et al, 2011;Porto et al, 2009Porto et al, , 2011Prica et al, 2006).…”

Section: In Vitro Biocompatibility Testingmentioning

confidence: 99%

Polymer Biocompatibility

Porto¹

2012

Polymerization

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HEMA reduces cell proliferation and induces apoptosis in vitro

Cited by 50 publications

References 32 publications

The influence of some iatrogenic factors on a cytomorphometric analysis of gingival cells and periodontal status among diabetic patients

The influence of some iatrogenic factors on a cytomorphometric analysis of gingival cells and periodontal status among diabetic patients

Uncompleted polymerization and cytotoxicity of dental restorative materials as potential health risk factors

Polymer Biocompatibility

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