Helicobacter pylori inactivation and virulence gene damage using a supported sensitiser for photodynamic therapy

Calvino-Fernández, Miryam; García‐Fresnadillo, David; Benito-Martínez, Selma; McNicholl, Adrián G.; Calvet, Xavier; Parra-Cid, T.

doi:10.1016/j.ejmech.2013.07.023

Cited by 16 publications

(5 citation statements)

References 41 publications

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“…The technique is based on light-induced ROS-production with the help of photosensitizing material. If the latter is positioned close to the target such as infectious bacteria, selective ROS-mediated toxicity may be obtained (14). Thus exploration of the redox homeostasis of H. pylori, preferentially in a physiological environment, would be helpful to characterize its sensitivity to ROS.…”

Section: -Extreme Extracellular Environments For Bacteria and Archaeamentioning

confidence: 99%

Environmental Effects on Reactive Oxygen Species Detection—Learning from the Phagosome

Nault

Bouchab

Dupre-Crochet

et al. 2016

Antioxidants & Redox Signaling

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Difficulties due to cell movement and continuous formation of new phagosomes can be reduced by ratio measurements, if appropriate dyes are identified. For detection in live cells and subcellular locations, fluorescent proteins (FPs) offer several advantages and are used to create biosensors for ROS. Some FPs are directly sensitive to certain ROS as shown here. Although this may compromise their use in an environment with high levels of ROS, it can also be exploited for ROS measurement directly with the FPs themselves. For all types of ROS detection, we suggest a set of basic guidelines for testing the environmental sensitivity of an ROS sensor. Antioxid. Redox Signal. 25, 564-576.

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Section: -Extreme Extracellular Environments For Bacteria and Archaeamentioning

confidence: 99%

Environmental Effects on Reactive Oxygen Species Detection—Learning from the Phagosome

Nault

Bouchab

Dupre-Crochet

et al. 2016

Antioxidants & Redox Signaling

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“…1,2 Chronic infection with H. pylori is implicated in the causation of various gastroduodenal diseases. 1,3,4 Antibiotic therapy is the standard treatment for H. pylori infection. However, the rapid emergence and dissemination of antibioticresistant strains has been documented globally in recent years.…”

Section: Introductionmentioning

confidence: 99%

“…Helicobacter pylori is a spiral‐shaped, Gram‐negative microaerophilic bacteria, which selectively colonizes the gastric and duodenal mucosa in humans. More than half of the world's population is believed to be infected with H. pylori . Chronic infection with H. pylori is implicated in the causation of various gastroduodenal diseases .…”

Section: Introductionmentioning

confidence: 99%

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Anti‐proliferation effect of blue light‐emitting diodes against antibiotic‐resistant Helicobacter pylori

Hiratsuka

Etoh

et al. 2018

J of Gastro and Hepatol

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Background and Aim Infection by Helicobacter pylori is implicated in a wide range of upper gastrointestinal diseases. Owing to the rapid emergence of antibiotic‐resistant strains of H. pylori, the development of novel treatment modalities for antibiotic‐resistant H. pylori infection is a key priority. Blue light‐emitting diodes (LED) may represent a unique option owing to their antimicrobial effect. In this study, we aimed to evaluate the anti‐proliferative effect of blue LED against antibiotic‐resistant H. pylori. Methods Ten antibiotic‐resistant strains and one sensitive H. pylori strain were used in this study. After irradiation by blue LED along time course, the viability of H. pylori was evaluated by enumerating colony forming units. Morphological changes in H. pylori were observed using a scanning electron microscope. Reductase activity was measured as an indicator of bacterial cellular activity. Total reactive oxygen species was monitored using fluorescence intensity and fluorescence microscope imaging. Results After irradiation by blue LED, the numbers of H. pylori in all the strains were significantly reduced compared with control group. The H. pylori exhibited a short rod‐shaped morphology after irradiation; no such change was observed in H. pylori not exposed to blue LED. Re‐irradiation of surviving strain after the initial irradiation also exhibited the same anti‐proliferation effect. After blue LED irradiation, bacterial cellular activity was lower, and total reactive oxygen species production was significantly higher in blue LED group, compared with that in control. Conclusions Blue LED could be a new treatment to eradicate infection with antibiotic‐resistant H. pylori.

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“…Resistance of H. pylori to different antibiotic regimens including amoxicillin, clarithromycin, metronidazole, levofloxacin, tetracycline, and rifampicin has been reported in regional studies from different countries [178][179][180][181] and is particularly causing chronic health problems in children [182]. Consequently, APDT has been employed as a possible, more patient-friendly alternative to conventional therapy [183][184][185].…”

Section: Helicobacter Pylori (Gram-negative)mentioning

confidence: 99%

Antibacterial photodynamic therapy: overview of a promising approach to fight antibiotic-resistant bacterial infections

et al. 2015

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Antibacterial photodynamic therapy (APDT) has drawn increasing attention from the scientific society for its potential to effectively kill multidrug-resistant pathogenic bacteria and for its low tendency to induce drug resistance that bacteria can rapidly develop against traditional antibiotic therapy. The review summarizes the mechanism of action of APDT, the photosensitizers, the barriers to PS localization, the targets, the in vitro-, in vivo-, and clinical evidence, the current developments in terms of treating Gram-positive and Gram-negative bacteria, the limitations, as well as future perspectives. Relevance for patients: A structured overview of all important aspects of APDT is provided in the context of resistant bacterial species. The information presented is relevant and accessible for scientists as well as clinicians, whose joint effort is required to ensure that this technology benefits patients in the post-antibiotic era.

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Helicobacter pylori inactivation and virulence gene damage using a supported sensitiser for photodynamic therapy

Cited by 16 publications

References 41 publications

Environmental Effects on Reactive Oxygen Species Detection—Learning from the Phagosome

Environmental Effects on Reactive Oxygen Species Detection—Learning from the Phagosome

Anti‐proliferation effect of blue light‐emitting diodes against antibiotic‐resistant Helicobacter pylori

Antibacterial photodynamic therapy: overview of a promising approach to fight antibiotic-resistant bacterial infections

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