Helicobacter pylori and cagA gene detected by polymerase chain reaction in gastric biopsies: correlation with histological findings, proliferation and apoptosis

Leite, Kátia Ramos Moreira; Darini, Elaine; Canavez, Flávio; Carvalho, Claudia M.; Mitteldorf, Cristina A.T.S.; Câmara-Lopes, Luiz H.

doi:10.1590/s1516-31802005000300005

Cited by 10 publications

(9 citation statements)

References 33 publications

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“…The rate of infection by this strain reported herein is similar to previous Brazilian studies of adult and infant populations published in recent years (6)(7)(8).…”

Section: Previous Reports That Suggesting That Infections By the H Psupporting

confidence: 90%

“…Brazilian patients suffering from various gastroduodenal diseases (6)(7)(8). The cagA virulence factor seems to be involved in the induction of proinflammatory chemokines expressed by host cells and plays a notable role in the onset and progression of gastroduodenal diseases (2,9).…”

Section: Previous Reports Have Shown That H Pylori Strains That Havementioning

confidence: 99%

“…pylori infections by strains carrying the cagA, vacA, iceA and babA2 genes were observed among Brazilian adults and children suffering from gastroduodenal diseases (6)(7)(8)18 America (21). Therefore, the consequent acquisition of babA2 and cagA genes would allow the H. pylori bacillus to adapt based on the prevalent ABO blood groups in South America (10).…”

Section: Previous Reports That Suggesting That Infections By the H Pmentioning

confidence: 99%

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ABO blood groups and Helicobacter pylori cagA infection: evidence of an association

Mattos¹,

Cintra²,

Mattos³

et al. 2010

J. Venom. Anim. Toxins incl. Trop. Dis

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Diseases resulting fromHelicobacter pylori infection appear to be dependent on a host of genetic traits and virulence factors possessed by this microorganism. This paper aimed to investigate the association between the ABO histo-blood groups and H. pylori cagA infections. Genomic DNA samples (n = 110) of gastric biopsies obtained from patients with endoscopic diagnosis of peptic ulcers (n = 25) and chronic active gastritis (n = 85) were analyzed by PCR using specific primers for the cagA gene. Of the samples, 66.4% (n = 73) tested positive and 33.6% (n = 37) negative for the gene. The cagA strain was predominant in peptic ulcers (n = 21; 84.0%) compared with chronic active gastritis (n = 52; 61.2%) (p = 0.05; OR 3.332; 95% CI: 1.050-10.576). Additionally, the cagA strain was prevalent in the type O blood (48/63; 76.2%) compared with other ABO phenotypes (25/47; 53.2%) (p = 0.01; OR 2.816; 95% CI: 1.246-6.364). These results suggest that H. pylori cagA infection is associated with the O blood group in Brazilian patients suffering from chronic active gastritis and peptic ulcers.

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“…The rate of infection by this strain reported herein is similar to previous Brazilian studies of adult and infant populations published in recent years (6)(7)(8).…”

Section: Previous Reports That Suggesting That Infections By the H Psupporting

confidence: 90%

Section: Previous Reports Have Shown That H Pylori Strains That Havementioning

confidence: 99%

Section: Previous Reports That Suggesting That Infections By the H Pmentioning

confidence: 99%

See 1 more Smart Citation

ABO blood groups and Helicobacter pylori cagA infection: evidence of an association

Mattos¹,

Cintra²,

Mattos³

et al. 2010

J. Venom. Anim. Toxins incl. Trop. Dis

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“…Similarly, Shiotani et al (16) found that H. pylori infection was associated with increased cell proliferation and apoptosis in GC patients but not the controls. However, van der Woude et al (27), Yoo et al (28), and Leite et al (29) found no significant differences between the AIs of H. pylori-positive subjects with GC and H. pylori-negative normal subjects. These findings do not seem to be clear-cut for the effect of H. pylori on these activities since many published reports were in disagreement.…”

Section: Discussionmentioning

confidence: 94%

Helicobacter pylori infection and relationship with gastric epithelial cell proliferation and apoptosis

Güçin¹,

Çakmak²,

Bayyurt³

et al. 2013

Turk J Med Sci

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A 2-year (2007 and 2008) open-field experiment was conducted to study the effects of irrigation on the yield parameters and main antioxidant components (lycopene, phenolic compounds, and ascorbic acid) of processing tomato. Two different treatments were applied: some plants were regularly irrigated, and some had their irrigation cut off 30 days before harvest. Both groups were compared with a rainfed control. Daily irrigation volume was calculated from the average daily temperature to reach optimum water supply. Fruits were harvested at the red-ripe maturity stage. The irrigated plants gave a significantly higher yield, and rainfed plants showed a yield loss. Irrigation had a greater effect on the average fruit weight than on fruit number. A seasonal effect was also remarkable, but was not as strong as that of irrigation. A better water supply caused a lower Brix number than that of the rainfed control. In both years, the antioxidant concentrations in tomato fruit showed a decrease with irrigation, except for the ascorbic acid content.

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“…Approximately 60%-80% of H. pylori strains express a high molecular weight protein, denominated CagA, coded by the cagA gene. CagA protein is translocated into the intracellular region of gastric epithelial cell, allowing the bacteria to modulate pathways of the cellular metabolism of the host (5,11) , inducing cellular hyper proliferation (19) , apoptosis (22) and leading to failure of gastric epithelial cell ability to maintain its normal cytoskeletal structure, an important prerequisite for neoplastic transformation (32) . Also, induce interleukin-8 (IL-8) production by the epithelial cells.…”

Section: H Pylori Was First Isolated In 1982 By Warren and Marshallmentioning

confidence: 99%

Relationship between caga-positive Helicobacter pylori infection and risk of gastric cancer: a case control study in Porto Alegre, RS, Brazil

Meine

Rota²,

Dietz

et al. 2011

Arq. Gastroenterol.

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-Context -Gastric cancer is the second most common cause of cancer related death worldwide. Although Helicobacter pylori has been classified as a class I carcinogen, the presence of infection is not a factor that alone is able to lead to gastric cancer, and one of the possible explanations for this is the existence of different strains of H. pylori with different degrees of virulence.Objectives -To investigate the association between cagA-positive H. pylori and gastric cancer, using polymerase chain reaction (PCR) for the detection of this bacterial strain. Methods -Twenty-nine patients with gastric cancer were matched by sex and age (± 5 years) with 58 patients without gastric cancer, submitted to upper gastrointestinal endoscopy. All patients were evaluated for the status of infection by H. pylori (through urease test, histological analysis and PCR for the genes ureA and 16SrRNA) and by cagA-positive strain (through PCR for cagA gene).

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Helicobacter pylori and cagA gene detected by polymerase chain reaction in gastric biopsies: correlation with histological findings, proliferation and apoptosis

Abstract: Cell growth deregulation in cagPAI+ patients could be demonstrated by the difference in the proliferation index. We believe that this explains the carcinogenic role of Helicobacter pylori.

Cited by 10 publications

References 33 publications

ABO blood groups and Helicobacter pylori cagA infection: evidence of an association

ABO blood groups and Helicobacter pylori cagA infection: evidence of an association

Helicobacter pylori infection and relationship with gastric epithelial cell proliferation and apoptosis

Relationship between caga-positive Helicobacter pylori infection and risk of gastric cancer: a case control study in Porto Alegre, RS, Brazil

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