HeLa Cell Adhesion on Various Collagen-Grafted Surfaces

Zhu, Yi; Wu, Zhonghan; Tang, Zuming; Zhang, Lu

doi:10.1021/pr020007a

Cited by 9 publications

(9 citation statements)

References 15 publications

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“…As a result of this difference, data generated from the dual-channel arrays were in terms of relative intensity values (i.e. ratio form) whereas data from the single-channel arrays were in terms of absolute intensity values (Zhu et al, 2002;Nakanishi et al, 2001). …”

Section: Dna Microarray Preparation Analysis and Subsequent Verificmentioning

confidence: 99%

“…An important cellular property in biotechnology applications is adhesion-a cell's ability to attach to a surface in order to grow (Zhu et al, 2002). Depending on the exact application, a cell line that does not adhere to a surface, anchorage-independent, may be strongly preferred over a cell line that adheres to a surface, anchorage-dependent (Lum et al, 2004;Karu et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…For a different application, an anchorage-dependent cell line may be preferable over an anchorage-independent cell line. Being able to manipulate the cellular feature of adhesion would, therefore, benefit biotechnology applications and is the basis of the current study (Lum et al, 2004;Zhu et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Application of microarrays to identify and characterize genes involved in attachment dependence in HeLa cells

Jaluria

Betenbaugh

Κωνσταντόπουλος

et al. 2007

Metabolic Engineering

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The ability to modify cellular properties such as adhesion is of interest in the design and performance of biotechnology-related processes. The current study was undertaken in order to evaluate the effectiveness of modulating cellular adhesion in HeLa cells from a genomics perspective. Using DNA microarrays, differences in gene expression between two phenotypically distinct, anchoragedependent and anchorage-independent, HeLa cell lines were identified. With the aid of several statistical methods and an extensive literature search, two genes were selected as potential targets for further study: siat7e and lama4.Subsequently, experiments were carried out to investigate the effects of siat7e and lama4 separately, on adhesion in HeLa cells by altering their expression in vivo. Decreasing the expression of siat7e, a type II membrane glycosylating sialyltransferase, in anchorage-independent HeLa cells using short interfering RNA (siRNA) resulted in greater aggregation (i.e. clumping) and morphological changes as compared to untreated anchorage-independent HeLa cells. Similar effects were seen in anchorageindependent HeLa cells when the expression of lama4 which encodes laminin α4, a member of the laminin family of glycoproteins, was enhanced as compared to untreated anchorage-independent HeLa cells. Using a shear flow chamber, an attachment assay was developed; illustrating either increased expression of siat7e or decreased expression of lama4 in anchorage-dependent HeLa cells reduced cellular adhesion.Collectively, the results of this study are consistent with the roles siat7e and lama4 play in adhesion processes in vivo and indicate modifying the expression of either gene can influence adhesion in HeLa cells. The strategy of applying bioinformatics techniques to characterize and manipulate phenotypic behaviors is a powerful tool for altering the properties of various cell lines for desired biotechnology objectives.

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Section: Dna Microarray Preparation Analysis and Subsequent Verificmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Application of microarrays to identify and characterize genes involved in attachment dependence in HeLa cells

Jaluria

Betenbaugh

Κωνσταντόπουλος

et al. 2007

Metabolic Engineering

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“…It has previously been used in studies aimed at optimizing cell adhesion to surfaces (27). KB cells are epithelial carcinoma cells that do not form tight junctions in monolayer culture.…”

Section: __________________________________________________________mentioning

confidence: 99%

In vitro assessment of the biocompatibility of chemically modified GaAs surfaces

Cho

Ivanisevic

2006

Nanobiotechnol

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GaAs has excellent optical, electrical, and mechanical properties and shows promise to be used in the fabrication of novel devices. However, the unprotected GaAs surface can release heavy metal compounds such as AsO x, which are toxic to living cells. A promising approach to reduce or eliminate this release relies on the passivation of the GaAs surface using different chemical approaches. In this work, we compared three different passivation methods aimed at enhancing the viability of cells on GaAs. Protective layers composed of self-assembled alkyl thiols, polypeptides, thick polymer layers, and shells of polyelectrolytes were tested. We confirmed that the GaAs surface can be made biocompatible for several days based on in vitro tests with HeLa and KB cells. In addition, we compared the cell spreading behavior on the GaAs substrates modified by different chemical approaches. Our results suggest that when the toxicity of the GaAs surface is reduced or eliminated, the cells' viability and spreading depend on the chemical and topographical nature of the surface.

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“…Interest in self-assembly of peptides and proteins has been motivated not only by a concern to understand the mechanisms of pathological aggregation but also to understand the functions and mechanisms of higher order assembly of proteins such as collagen. Natural collagen has been found useful in enhancing cell adhesion [26], tissue regeneration [27][28][29], and drug delivery [30,31]; therefore, scaffolds and biomaterials mimicking the natural collagen and enhancing its biological functions have been generated [32][33][34][35][36].…”

Section: Supramolecular Nanostructure Formation Inspired By Naturementioning

confidence: 99%

Self-assembled peptidic nanostructures

Toksöz

Güler

2009

Nano Today

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Cataloged from PDF version of article.Understanding the mechanisms of molecular self-assembly processes in natural\ud materials is an important step to developing new functional materials with useful properties.\ud Recently, various self-assembled materials have gained attention because of their interesting\ud properties in nanoscale. Nanostructures composed of peptides are especially of interest\ud in materials development because of their many advantageous properties such as biodegradability,\ud biocompatibility and customizable bioactivity. Self-assembled peptidic nanostructures\ud have been studied by many research groups and found use as three-dimensional cell scaffolds\ud for bone regeneration, dental implants, neural tissue engineering, biosensors for detection of\ud viruses and other pathogens, antibacterial agents and in drug, protein and gene delivery

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HeLa Cell Adhesion on Various Collagen-Grafted Surfaces

Cited by 9 publications

References 15 publications

Application of microarrays to identify and characterize genes involved in attachment dependence in HeLa cells

Application of microarrays to identify and characterize genes involved in attachment dependence in HeLa cells

In vitro assessment of the biocompatibility of chemically modified GaAs surfaces

Self-assembled peptidic nanostructures

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