Heavy metal resistance to the extreme: Streptomyces strains from a former uranium mining area

Schmidt, André; Haferburg, Götz; Schmidt, André; Li, Lei; Merten, D.; Ghergel, Felicia; Büchel, Georg; Kothe, Erika

doi:10.1016/j.chemer.2007.11.002

Cited by 55 publications

(41 citation statements)

References 32 publications

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“…and Streptomyces spp. is not surprising in heavymetal contaminated soil and their occurrence in this polluted environment was confirmed by several authors (Joynt et al 2006;Schmidt et al 2009;Field et al 2010;El-Meleigy et al 2011;Karelová et al 2011;Lin et al 2011;Al-Kadeeb et al 2012). Similarly, the occurrence of the bacterial subclass Rubrobacteridae clones is not surprising as well, because 70% of the actinobacterial clones belonged to the genus Rubrobacter, a widespread taxa that has yet few cultured representatives in soils (Holmes et al 2000).…”

Section: Resultssupporting

confidence: 52%

Actinobacteria occurrence and their metabolic characteristics in the nickel-contaminated soil sample

et al. 2014

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Heavy metals are a significant source of pollution in soils that have been demonstrated to exert significant toxic effect on soil microbial assemblages. Here we investigate the occurrence and metabolic characteristics of actinobacteria, which form a predominated component of farmland bacterial community near the town of Sereď in southwest Slovakia, contaminated by close nickel ore facility. Actinobacteria occurred in this environment with high concentrations of nickel (2.109 mg/kg), slightly above the natural occurrence of cobalt (355 mg/kg) and zinc (177 mg/kg), even too low concentration of iron (35.75 mg/kg) for a normal soil and not a toxic amount of copper (32.2 mg/kg) and cadmium (<0.25 mg/kg). The phylogeny was reconstructed using partial sequences of 16S rDNA genes of both, actinobacterial isolates and clones. A total of 105 actinobacterial representatives were divided into 66 species belonging to one order, 7 genera and 5 uncategorized groups. The selected 14 morphologically distinct isolates were able to produce drop collapsing, haemolytic and lipase activities. Whereas only 4 isolates produced dark brown melanin pigment and only 5 of them produced decolourization of the azo dye, all isolates tested were capable of assimilating all 11 sugars tested. All these actinobacterial isolates were resistant to nickel, cobalt, zinc, cadmium, copper, but the level of resistance differed between the individual isolates, and the resistance profiles of antibiotics (gentamycin, ampicillin, ciprofloxacin, chloramphenicol, erythromycin, rifampicin, penicillin-G) varied among them to a certain extent. Our results suggested that actinobacteria in soil contaminated by nickel present a relatively divergent group inside of microbial assemblage.

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Section: Resultssupporting

confidence: 52%

Actinobacteria occurrence and their metabolic characteristics in the nickel-contaminated soil sample

et al. 2014

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“…Schmidt et al (2009) observed that bacterial isolates identified as the same species, respond to nickel using difcontaining trace metal amendments than in media lacking such amendments (Table 1). Only three of the eight isolates inhibited S. aureus growth on any of the complex medias, while seven of the eight isolates inhibited the growth of S. aureus on at least one of the trace metal-amended mediums.…”

Section: Discussionmentioning

confidence: 98%

Addition of Zinc, Manganese, and Iron to Growth Media Triggers Antibiotic Production in Bacterial Isolates From the Lower Atmosphere

Washburn¹,

Spradlin²,

Weber³

2017

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“…The strain Streptomyces mirabilis P16B-1, which was isolated from the former uranium-mining site WISMUT, Ronneburg, Germany, is known to be extremely heavy metal-resistant (Schmidt et al, 2008) and to grow directly in heavy metal-contaminated soil without addition of any media components (Schütze and Kothe, 2012). Streptomyces lividans TK24 was used as a heavy metal-sensitive control strain (Ravel et al, 2000;Amoroso et al, 2002).…”

Section: Strains and Culture Conditionsmentioning

confidence: 99%

“…We applied heavy metal-contaminated soil, and the two Streptomyces strains had different sensitivity towards heavy metals. In this controlled system, the heavy metal-sensitive bacterial strain Streptomyces lividans TK24 would die and lead to formation of SOM, while the resistant strain, S. mirabilis P16B-1 (Schmidt et al, 2008), was expected to survive or even grow without measurable contribution to SOM formation. Also the fate of cytosolic proteins was studied using the example of the antioxidant enzyme nickel-containing superoxide dismutase (NiSOD), which might be one of the heavy metal resistance-mediating proteins in streptomycetes (Schmidt et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Live and death of streptomyces in soil—what happens to the biomass?

Schütze

Miltner

Nietzsche

et al. 2013

Z. Pflanzenernähr. Bodenk.

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The formation of soil organic matter (SOM) has been proposed to depend on fragmentation of biomass after cell death. However, this is hard to mimic in laboratory experiments showing the process directly. We used heavy metal contamination in order to provide an environment in which one Streptomyces strain, the heavy metal resistant S. mirabilis P16B-1, could survive while the sensitive strain S. lividans TK24 was expected to die and disintegrate; the necromass fragments would then contribute to SOM formation. Both strains were grown for 30 d in sterile mesocosms containing either highly metal-contaminated soil from a former uranium-mining site in Ronneburg, Germany, or control soil from a municipal park, Jena, Germany. The fate and morphology of living and dead bacterial biomass (necromass) was observed using scanning electron microscopy. Attachment of soil particles to the intact mycelium as well as decay of dead biomass was observed. Dead bacterial biomass was identified in form of patchy fragments while the superordinate filamentous structure of the hyphae was still visible and obviously stabilized in soil. The fate of cytosolic compounds was followed using the example of a nickel-containing superoxide dismutase (NiSOD) which was found to be released after death of cells grown in liquid soil-extract medium. Activity of the enzyme was proven for concentrated media supernatant by a gel-based qualitative activity assay. This indicates that NiSOD remains active in soil after cell death. Hence, bacterial cell death results in the release of cytosolic compounds, e.g., intact proteins, as well as the formation of residual cell-envelope fragments contributing to SOM formation.

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Heavy metal resistance to the extreme: Streptomyces strains from a former uranium mining area

Cited by 55 publications

References 32 publications

Actinobacteria occurrence and their metabolic characteristics in the nickel-contaminated soil sample

Actinobacteria occurrence and their metabolic characteristics in the nickel-contaminated soil sample

Addition of Zinc, Manganese, and Iron to Growth Media Triggers Antibiotic Production in Bacterial Isolates From the Lower Atmosphere

Live and death of streptomyces in soil—what happens to the biomass?

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