Heavy metal resistance mechanisms in actinobacteria for survival in AMD contaminated soils

Schmidt, André; Haferburg, Götz; Siñeriz, Manuel; Merten, D.; Büchel, Georg; Kothe, Erika

doi:10.1016/j.chemer.2005.06.006

Cited by 113 publications

(68 citation statements)

References 19 publications

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“…Similarly, Vivas et al (2008) found high dominance indices in the other more polluted soils including heavy metals, indicating the supremacy of populations, which may be metabolically more active due to the presence of pollutants. However, microbial activity and count of spore-producing bacteria decreased in soil with high heavy metal concentrations (Schmidt et al 2005). Hiroki (1992) furhter suggested that yet the relatively low concentrations of heavy metals in heavy-metal contaminated soils affected the soil microbial population.…”

Section: Resultsmentioning

confidence: 99%

Actinobacteria occurrence and their metabolic characteristics in the nickel-contaminated soil sample

et al. 2014

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Heavy metals are a significant source of pollution in soils that have been demonstrated to exert significant toxic effect on soil microbial assemblages. Here we investigate the occurrence and metabolic characteristics of actinobacteria, which form a predominated component of farmland bacterial community near the town of Sereď in southwest Slovakia, contaminated by close nickel ore facility. Actinobacteria occurred in this environment with high concentrations of nickel (2.109 mg/kg), slightly above the natural occurrence of cobalt (355 mg/kg) and zinc (177 mg/kg), even too low concentration of iron (35.75 mg/kg) for a normal soil and not a toxic amount of copper (32.2 mg/kg) and cadmium (<0.25 mg/kg). The phylogeny was reconstructed using partial sequences of 16S rDNA genes of both, actinobacterial isolates and clones. A total of 105 actinobacterial representatives were divided into 66 species belonging to one order, 7 genera and 5 uncategorized groups. The selected 14 morphologically distinct isolates were able to produce drop collapsing, haemolytic and lipase activities. Whereas only 4 isolates produced dark brown melanin pigment and only 5 of them produced decolourization of the azo dye, all isolates tested were capable of assimilating all 11 sugars tested. All these actinobacterial isolates were resistant to nickel, cobalt, zinc, cadmium, copper, but the level of resistance differed between the individual isolates, and the resistance profiles of antibiotics (gentamycin, ampicillin, ciprofloxacin, chloramphenicol, erythromycin, rifampicin, penicillin-G) varied among them to a certain extent. Our results suggested that actinobacteria in soil contaminated by nickel present a relatively divergent group inside of microbial assemblage.

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Section: Resultsmentioning

confidence: 99%

Actinobacteria occurrence and their metabolic characteristics in the nickel-contaminated soil sample

et al. 2014

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“…These taxa have also been connected to bulk terrestrial soils contaminated with Ni, Co, and Zn (Harichova et al 2012) and hydrocarbons (Sutton et al 2013). Actinobacteria have been isolated and observed to possess mechanisms promoting resistance to heavy metal disturbance (Schmidt et al 2005), and have also been isolated from contaminated subsurface sediments (Bollmann et al 2010). However, among all the phyla, Gemmatimonates and Actinobacteria were the only phyla found to be present in metal-associated aquatic sediments prior to this study (Besaury et al 2013;Reis et al 2013).…”

Section: Community Comparison Of Aquatic Sedimentsmentioning

confidence: 98%

High concentrations of bioavailable heavy metals impact freshwater sediment microbial communities

Horton

Rui

et al. 2015

Ann Microbiol

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Heavy metal concentrations within freshwater systems can increase as a result of anthropogenic inputs, which in turn can influence microbial community composition and community function. In this study, lake sediments collected from two historically heavy metal impacted geographical regions [Poyang Lake, People's Republic of China (PRC), and sites adjacent to the Muskegon Watershed, MI (USA)] were analyzed for total and available heavy metals (Cd, Cr, Cu, Pb, and Zn), as well as microbial community structure using highthroughput sequencing technology. In PRC tributaries leading into Poyang Lake, Zn was found to be the most available metal species. In USA sites, available Cd was higher in Mona Lake sites than alternate USA sites. In both regions, results indicate a weak influence of metals on microbial alpha and beta diversity, but strong positive and negative correlations of available heavy metals with specific taxonomic groups of bacteria and archaea. Further, individual metal species impacted microbial groups and operational taxonomic units differently, suggesting that individual metal species are important in determining which microbial groups and species prevail under heavy metal stress, particularly in the case of bioavailable metals. In PRC, several taxonomic groups were correlated positively with Zn, including Actinobacteria, Bacteroidetes, Proteobacteria, and Verrucomicrobia. Geobacter and Pedosphaerales were both correlated positively to Cr in the same sites, while other correlations with microbial groups were negative. In sites from the USA, Deltaproteobacteria were correlated positively with Cd, while Betaproteobacteria were negatively correlated, suggesting that Cd resistance may differ at the level of Class within Proteobacteria. This study highlights the importance of analyzing available metals when examining the impact of this disturbance on microbial community structure within aquatic sediments.

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“…It has been shown by many authors that heavy metal contamination negatively affects production of antibiotics and pigments by Streptomyces species (Abbas and Edward, 1989). Schmidt et al (2005) reported inhibition by cadmium of antibiotic production and total growth of Streptomyces species. The same result was previously reported by Abbas and Edward (1989), showing high toxicity to streptomyces of not only cadmium but also mercury, nickel, copper and lead.…”

Section: Actinobacteria and Metalsmentioning

confidence: 99%

“…Since they consist of reactive heavy metals, such abilities are logically encountered more readily among microbes living in contaminated environment than in others because bacteria may activate and adapt a mechanism of detoxification to ensure survival (Eitinger and Mandrand-Berthelot, 2000). Schmidt et al (2005) isolated actinobacterial strains belonging to the genus Streptomyces showing multitude of responses to metal contamination. One spectacular trait is the release of diffusible substance (not characterized in the published work) that allows not only resistance of the producer strain, but also of adjacent strains in the culture media.…”

Section: Actinobacteria and Metalsmentioning

confidence: 99%

Actinobacteria Isolation from Metal Contaminated Soils for Assessment of their Metal Resistance and Plant Growth Promoting (PGP) Characteristics

Tekaya¹,

Tipayno

Chandrasekaran³

et al. 2012

Korean Journal of Soil Science and Fertilizer

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Heavy metals and metalloids removal can be considered as one of the most important world challenges because of their toxicity and direct impact on human health. Many processes have been introduced but biological processes of remediation seem to offer the most suitable solution in terms of efficiency and low cost. Actinobacteria constitute one of the major microbial populations in soil, and this can be attributed to their adaptive morphological structure as well as their exceptional metabolic power. Among microbes, actinobacteria are morphologic intermediate between fungi and bacteria. Studies on microbial diversities in metal contaminated lands have shown that actinobacteria may constitute a dominantly active microbiota in addition to α Proteobacteria. Furthermore, isolation studies have shown metal removal mechanisms which are reminiscent of notable multiresistant strains, such as Cupriavidus metallidurans. Apart from members of genus Streptomyces, which produce more than 90% of commercialized antibiotics, and the nitrogen fixing Frankia, little attention has been given to other members of this phylum. This is because of difficult culture condition requirements and maintenance. In this review, we focused on specific isolation of actinobacteria and their potential applications in metal bioremediation and plant growth promotion.

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Heavy metal resistance mechanisms in actinobacteria for survival in AMD contaminated soils

Cited by 113 publications

References 19 publications

Actinobacteria occurrence and their metabolic characteristics in the nickel-contaminated soil sample

Actinobacteria occurrence and their metabolic characteristics in the nickel-contaminated soil sample

High concentrations of bioavailable heavy metals impact freshwater sediment microbial communities

Actinobacteria Isolation from Metal Contaminated Soils for Assessment of their Metal Resistance and Plant Growth Promoting (PGP) Characteristics

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