Heavy metal‐associated isoprenylated plant protein (HIPP): characterization of a family of proteins exclusive to plants

Abstract

Metallochaperones are key proteins for the safe transport of metallic ions inside the cell. HIPPs (heavy metal-associated isoprenylated plant proteins) are metallochaperones that contain a metal binding domain (HMA) and a C-terminal isoprenylation motif. In this study, we provide evidence that proteins of this family are found only in vascular plants and may be separated into five distinct clusters. HIPPs may be involved in (a) heavy metal homeostasis and detoxification mechanisms, especially those involved in cadmium tolerance, (b) transcriptional responses to cold and drought, and (c) plant-pathogen interactions. In particular, our results show that the rice (Oryza sativa) HIPP OsHIPP41 gene is highly expressed in response to cold and drought stresses, and its product is localized in the cytosol and the nucleus. The results suggest that HIPPs play an important role in the development of vascular plants and in plant responses to environmental changes.

Potato Mop-Top Virus Co-Opts the Stress Sensor HIPP26 for Long-Distance Movement

“…HIPPs have been variously clustered into five (de Abreu-Neto et al, 2013), six (Barth et al,128 2009) and seven major groups (Tehseen et al, 2010). that are post-translationally lipid modified by the addition of a prenyl group to enable 141 membrane association (reviewed in Crowell, 2000).…”

“…Plant 145 proteins in the HIPP family all contain a CaaX motif and are all thought to be prenylated (de 146 Abreu- Neto et al, 2013). Furthermore, a strong prenylation prediction was obtained when 147 the NbHIPP26 sequence was analysed using Prenylation Prediction Suite software (Score = 148 2.514, P = 0.000554; Maurer-Stroh and Eisenhaber, 2005).…”

Comparative Transcriptional Profiling of Two Wheat Genotypes, with Contrasting Levels of Minerals in Grains, Shows Expression Differences during Grain Filling

“…MT is a cystein rich protein having the capacity to bind with metals through its thiol group. It plays an essential role in metal ion homeostasis, transport and storage in the cell [32], [33], [34]. In the seed, its abundance has been reported in aleurone and embryo [35], whereas endosperm shows comparatively less expression.…”

“…Elevated transcription of Manganese ion and Transition metal ion binding proteins was observed in IITR26, mainly during 14 DAA (Table 3). Transition metal binding protein, an orthologue of Farnesylated protein 3, is involved in executing metal ion homeostasis and, biotic and abiotic stress tolerance [34]. Its expression has been recorded mostly in seed tissues (Figure 4).…”

ceRNA Cross-Talk in Paulownia Witches’ Broom Disease

“…Genes encoding heavy metal associated isoprenylated plant proteins (HIPPs, EVM0015501.1), which are metallochaperones, were found in this part of the network. HIPP may be involved in various roles in plant development and defense responses [54]. …”