Comparative Transcriptional Profiling of Two Wheat Genotypes, with Contrasting Levels of Minerals in Grains, Shows Expression Differences during Grain Filling

Abstract

Wheat is one of the most important cereal crops in the world. To identify the candidate genes for mineral accumulation, it is important to examine differential transcriptome between wheat genotypes, with contrasting levels of minerals in grains. A transcriptional comparison of developing grains was carried out between two wheat genotypes- Triticum aestivum Cv. WL711 (low grain mineral), and T. aestivum L. IITR26 (high grain mineral), using Affymetrix GeneChip Wheat Genome Array. The study identified a total of 580 probe sets as differentially expressed (with log2 fold change of ≥2 at p≤0.01) between the two genotypes, during grain filling. Transcripts with significant differences in induction or repression between the two genotypes included genes related to metal homeostasis, metal tolerance, lignin and flavonoid biosynthesis, amino acid and protein transport, vacuolar-sorting receptor, aquaporins, and stress responses. Meta-analysis revealed spatial and temporal signatures of a majority of the differentially regulated transcripts.

Unveiling the transcriptome complexity of the High- and Low- Zinc & Iron accumulating Indian wheat (Triticum aestivum L.) cultivars

“…Current study also deals with comparative transcriptome analysis of 7 genotypes of low- and high- Zn & Fe accumulating wheat, contrasting the reason for grain mineral concentration by recognizing various DEGs associated in the uptakes of minerals ( Figure 1 ). Results provide better understanding on metal homeostasis, metal tolerance, flavonoid biosynthesis, micronutrient, amino acid & protein transport and various stress responses in comparison earlier performed study by Singh et al 2014 [Singh et al 2014]. Prevalence of genes encoding proteins related Chlorophyll synthesis, Metal binding, Metal ion transport and ATP-Synthase coupled transport and stress responses, was noticed high in HZFWGs [Sperotto et al 2014; Sperotto et al 2018].…”

Genome-Wide Association Study Reveals Novel Genomic Regions Associated with 10 Grain Minerals in Synthetic Hexaploid Wheat

“…Of these, 28 MTAs were present in 19 genes whose annotations indicate they are associated with grain minerals ( Table 3 and Table S4 ). For instance, MTAs for Fe were located in genes related to Fe concentration such as 2-oxoglutarate (2OG), Fe(II)-dependent oxygenase superfamily protein [ 32 , 33 ], ATP synthase gamma chain [ 34 ], F-box family protein domain [ 33 ], GDSL esterase/lipase [ 35 ], leucine-rich receptor-like protein kinase [ 36 , 37 ], Myb transcription factor [ 36 , 37 ], Na-translocating NADH–quinone reductase subunit A [ 38 ], no apical meristem (NAM) protein [ 39 ], protein DETOXIFICATION [ 37 ], ROP guanine nucleotide exchange factor 10 [ 33 ], and universal stress protein family [ 37 ]. Additional examples are provided in Table 3 .…”

Genetic dissection of seed-iron and zinc concentrations in chickpea

“…The seed-specific expression, including a pronounced upregulation and enhanced transcript accumulation of three strong seed-Fe and Zn contents-associated known/candidate genes [encoding LEA, YSL1 and vacuolar protein sorting-associated proteins] have ascertained significant involvement of these genes in storing high level of Fe and Zn contents in the maturing seeds of multiple crop (legumes) accessions 46 47 48 58 59 60 . These genes with useful properties of Fe/Zn-binding, are known to be involved in micronutrients trafficking, signalling and storage, especially overall homeostasis of Fe and Zn contents in seeds of crop plants.…”