Heavy ion microprobes: a unique tool for bystander research and other radiobiological applications

Voss, Kay-O.; Fournier, Claudia; Taucher-Scholz, G.

doi:10.1088/1367-2630/10/7/075011

Cited by 14 publications

(9 citation statements)

References 82 publications

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“…Similar images to Fig. 8B have already been reported using the heavy ion microbeam of GSI, Darmstadt, after immuno-staining of cell nuclei against 53BP1 or γ-H2AX [27, 28]. It should be noted that heavy ions are advantageous for these experiments because they are less readily scattered, and particle detection is less problematic in the microbeam technique.…”

Section: Resultssupporting

confidence: 77%

SPICE-NIRS Microbeam: a focused vertical system for proton irradiation of a single cell for radiobiological research

Konishi¹,

Oikawa²,

Suya³

et al. 2013

Journal of Radiation Research

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The Single Particle Irradiation system to Cell (SPICE) facility at the National Institute of Radiological Sciences (NIRS) is a focused vertical microbeam system designed to irradiate the nuclei of adhesive mammalian cells with a defined number of 3.4 MeV protons. The approximately 2-μm diameter proton beam is focused with a magnetic quadrupole triplet lens and traverses the cells contained in dishes from bottom to top. All procedures for irradiation, such as cell image capturing, cell recognition and position calculation, are automated. The most distinctive characteristic of the system is its stability and high throughput; i.e. 3000 cells in a 5 mm × 5 mm area in a single dish can be routinely irradiated by the 2-μm beam within 15 min (the maximum irradiation speed is 400 cells/min). The number of protons can be set as low as one, at a precision measured by CR-39 detectors to be 99.0%. A variety of targeting modes such as fractional population targeting mode, multi-position targeting mode for nucleus irradiation and cytoplasm targeting mode are available. As an example of multi-position targeting irradiation of mammalian cells, five fluorescent spots in a cell nucleus were demonstrated using the γ-H2AX immune-staining technique. The SPICE performance modes described in this paper are in routine use. SPICE is a joint-use research facility of NIRS and its beam times are distributed for collaborative research.

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Section: Resultssupporting

confidence: 77%

SPICE-NIRS Microbeam: a focused vertical system for proton irradiation of a single cell for radiobiological research

Konishi¹,

Oikawa²,

Suya³

et al. 2013

Journal of Radiation Research

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“…GSI (Gesellschaft für Schwerionenforschung) in Darmstadt (Germany) has a heavy ion microprobe that focuses the beam to ~0.7 × 0.5 µm [16]. These facilities both produce ion beams of light ions (protons and helium ions) in addition to beams of heavy ions.…”

Section: Discussionmentioning

confidence: 99%

The Columbia University sub-micron charged particle beam

Randers-Pehrson

Johnson

Marino

et al. 2009

Nuclear Instruments and Methods in Physics Research Section A:

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A lens system consisting of two electrostatic quadrupole triplets has been designed and constructed at the Radiological Research Accelerator Facility (RARAF) of Columbia University. The lens system has been used to focus 6-MeV 4He ions to a beam spot in air with a diameter of 0.8 µm. The quadrupole electrodes can withstand voltages high enough to focus 4He ions up to 10 MeV and protons up to 5 MeV. The quadrupole triplet design is novel in that alignment is made through precise construction and the relative strengths of the quadrupoles are accomplished by the lengths of the elements, so that the magnitudes of the voltages required for focusing are nearly identical. The insulating sections between electrodes have had ion implantation to improve the voltage stability of the lens. The lens design employs Russian symmetry for the quadrupole elements.

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“…(i) precision microbeams that can selectively target a preset fraction of cells each with an exact number of particles (Figure 2A). At present, heavy-ion microbeams are available for biological studies at three facilities: microbeams are collimated through microapertures at the Japan Atomic Energy Agency (Gunma, Japan), and focused with magnetic lenses at the Gesellschaft für Schwerionenforschung (Darmstadt, Germany) and the Technische Universität München (Munich, Germany) Kobayashi et al, 2004Kobayashi et al, , 2009Voss et al, 2008). (ii) broadbeams that deliver very low fluences/doses of particles where the fraction of irradiated cells follows a Poisson distribution ( Figure 2B).…”

Section: Irradiation Systemmentioning

confidence: 99%

The Bystander Response to Heavy-Ion Radiation: Intercellular Signaling Between Irradiated and Non-Irradiated Cells

Hamada

2009

Biol. Sci. Space

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Ionizing radiation-induced bystander effect is the phenomenon whereby cells that are not themselves irradiated but received signals from directly irradiated cells exhibit biological r e s p o n s e s . H u m a n e x p o s u r e t o h e av y -ion radiation occurs during manned space missions at the dose and dose rate much lower than those for cancer therapy. It should be noted herein that less irradiated cells coexist with more non-irradiated counterparts in a cell population exposed to a lower dose of radiation whose linear energy transfer is higher. The bystander effect should be thence examined to decipher the action mechanism of low-dose heavy ions. The bystander effect of heavy ions is manifested as inactivated clonogenic potential, a transient apoptotic response, delayed p53 phosphorylation, gene expression changes, a transient cell-cycle arrest, chromosome aberrations, elevated mutation fre quency and micronucleation. Proposed underpinning mechanisms involve gap junctional intercellular communication and reactive oxygen/nitrogen species. This paper reviews briefly the current knowledge of the heavy ion-induced bystander effect.The bystander effect of heavy-ion radiation − 196 −

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Heavy ion microprobes: a unique tool for bystander research and other radiobiological applications

Cited by 14 publications

References 82 publications

SPICE-NIRS Microbeam: a focused vertical system for proton irradiation of a single cell for radiobiological research

SPICE-NIRS Microbeam: a focused vertical system for proton irradiation of a single cell for radiobiological research

The Columbia University sub-micron charged particle beam

The Bystander Response to Heavy-Ion Radiation: Intercellular Signaling Between Irradiated and Non-Irradiated Cells

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