The Columbia University sub-micron charged particle beam

Randers-Pehrson, Gerhard; Johnson, Gary W.; Marino, Stephen; Xu, Yanping; Dymnikov, Alexander D.; Brenner, David J.

doi:10.1016/j.nima.2009.08.041

Cited by 33 publications

(43 citation statements)

References 16 publications

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“…The two triplets were positioned in a long metal tube and aligned in our shop before the tube was placed in the beamline. In 2008 the compound lens was able to focus the 4 He beam to 0.8 μm – a submicron beam (29). One of our collaborators (David Chen) developed a human HT1080 fibrosarcoma cell line in which the cells have been transfected with a XRCC1 DNA single-strand break repair protein tagged with green fluorescent protein.…”

Section: Irradiation Facilitiesmentioning

confidence: 99%

50 Years of the Radiological Research Accelerator Facility (RARAF)

Marino

2017

Radiation Research

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The Radiological Research Accelerator Facility (RARAF) is in its 50th year of operation. It was commissioned on April 1, 1967 as a collaboration between the Radiological Research Laboratory (RRL) of Columbia University, and members of the Medical Research Center of Brookhaven National Laboratory (BNL). It was initially funded as a user facility for radiobiology and radiological physics, concentrating on monoenergetic neutrons. Facilities for irradiation with MeV light charged particles were developed in the mid-1970s. In 1980 the facility was relocated to the Nevis Laboratories of Columbia University. RARAF now has seven beam lines, each having a dedicated irradiation facility: monoenergetic neutrons, charged particle track segments, two charged particle microbeams (one electrostatically focused to <1 μm, one magnetically focused), a 4.5 keV soft X-ray microbeam, a neutron microbeam, and a facility that produces a neutron spectrum similar to that of the atomic bomb dropped at Hiroshima. Biology facilities are available on site within close proximity to the irradiation facilities, making the RARAF very user friendly.

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Section: Irradiation Facilitiesmentioning

confidence: 99%

50 Years of the Radiological Research Accelerator Facility (RARAF)

Marino

2017

Radiation Research

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“…Beam size and beam location were measured with a nickel knife edge scan (Randers-Pehrson et al 2009). Beam location was registered using scanning of fluorescent beads.…”

Section: Measurementmentioning

confidence: 99%

Metabolic oxygen consumption measurement with a single-cell biosensor after particle microbeam irradiation

Zhang

Messerli

et al. 2014

Radiat Environ Biophys

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A noninvasive, self-referencing biosensor/probe system has been integrated into the Columbia University Radiological Research Accelerator Facility Microbeam II end station. A single-cell oxygen consumption measurement has been conducted with this type of oxygen probe in 37°C Krebs–Ringer Bicarbonate buffer immediately before and after a single-cell microbeam irradiation. It is the first such measurement made for a microbeam irradiation, and a six fold increment of oxygen flux induced during a 15-s period of time has been observed following radiation exposure. The experimental procedure and the results are discussed.

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“…The advantage of single ion microprobes is their ability to deliver known doses in precise locations within a cell or population of cells. In the early 1990’s, the Radiological Research Accelerator Facility at Columbia University was one of the first facilities to build a single ion microbeam [4, 5], and today there are over 30 radiobiological microbeams globally [6]. …”

Section: Introduction/backgroundmentioning

confidence: 99%

“…Currently RARAF uses a 300 μm thick solid state detector (ORTEC model R-015-050-300, Oak Ridge, TN) [5]. With this thickness detector, incident alpha particles will deposit all their energy and will be stopped by the detector.…”

Section: Introduction/backgroundmentioning

confidence: 99%

“…The average energy to create an ion pair in a gas is 15–30, roughly 5–10 times more than the materials used in semiconductor detectors, where the energy required to create an electron-hole pair in silicon is 3.62 eV. RARAF currently has a custom built gas counter that has an 8 mm long gas ionization chamber [5]. Commercial semiconductor detectors are available from companies such as ORTEC, and are reverse biased diodes that rely on the generation of electron-hole pairs by charged particles.…”

Section: Introduction/backgroundmentioning

confidence: 99%

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An ultra-thin Schottky diode as a transmission particle detector for biological microbeams

et al. 2012

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We fabricated ultrathin metal-semiconductor Schottky diodes for use as transmission particle detectors in the biological microbeam at Columbia University’s Radiological Research Accelerator Facility (RARAF). The RARAF microbeam can deliver a precise dose of ionizing radiation in cell nuclei with sub-micron precision. To ensure an accurate delivery of charged particles, the facility currently uses a commercial charged-particle detector placed after the sample. We present here a transmission detector that will be placed between the particle accelerator and the biological specimen, allowing the irradiation of samples that would otherwise block radiation from reaching a detector behind the sample. Four detectors were fabricated with co-planar gold and aluminum electrodes thermally evaporated onto etched n-type crystalline silicon substrates, with device thicknesses ranging from 8.5 μm – 13.5 μm. We show coincident detections and pulse-height distributions of charged particles in both the transmission detector and the commercial detector above it. Detections are demonstrated at a range of operating conditions, including incoming particle type, count rate, and beam location on the detectors. The 13.5 μm detector is shown to work best to detect 2.7 MeV protons (H+), and the 8.5 μm detector is shown to work best to detect 5.4 MeV alpha particles (4He++). The development of a transmission detector enables a range of new experiments to take place at RARAF on radiation-stopping samples such as thick tissues, targets that need immersion microscopy, and integrated microfluidic devices for handling larger quantities of cells and small organisms.

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The Columbia University sub-micron charged particle beam

Cited by 33 publications

References 16 publications

50 Years of the Radiological Research Accelerator Facility (RARAF)

50 Years of the Radiological Research Accelerator Facility (RARAF)

Metabolic oxygen consumption measurement with a single-cell biosensor after particle microbeam irradiation

An ultra-thin Schottky diode as a transmission particle detector for biological microbeams

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