Heavy Ion Effects on Cellular DNA: Strand Break Induction and Repair in Cultured Diploid Lens Epithelial Cells

Aufderheide, Enno; Rink, H.; Hieber, Ludwig; Kraft, Gerhard

doi:10.1080/09553008714551071

Cited by 42 publications

(15 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…IR kills cells primarily by inducing DNA double strand breaks (DSBs). Because high LET and low LET radiation generate similar numbers of initial DSBs per unit of absorption, the RBE for high LET radiation in cell killing arises from the fact that DSBs are more difficult to repair (2,3); however, the underlying causes are not fully understood.…”

Section: Examples Of High Linear Energy Transfer (Let)mentioning

confidence: 99%

Distinct Roles of Ape1 Protein, an Enzyme Involved in DNA Repair, in High or Low Linear Energy Transfer Ionizing Radiation-induced Cell Killing

Wang

Chen

et al. 2014

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

Section: Examples Of High Linear Energy Transfer (Let)mentioning

confidence: 99%

Distinct Roles of Ape1 Protein, an Enzyme Involved in DNA Repair, in High or Low Linear Energy Transfer Ionizing Radiation-induced Cell Killing

Wang

Chen

et al. 2014

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…Ritter et al . (1977), Aufderheide et al . (1987) andHeilmann et al (1993) found an increased fraction of unrejoinable strand breaks after heavy ion irradia- Figure 11 .…”

Section: 5 Comparison With Other Biological Endpointsmentioning

confidence: 94%

Cell Cycle Delays Induced by Heavy Ion Irradiation of Synchronous Mammalian Cells

Scholz¹,

Kraft-Weyrather²,

Ritter³

et al. 1994

International Journal of Radiation Biology

Self Cite

View full text Add to dashboard Cite

Cell cycle delays in V79 Chinese hamster cells induced by heavy ion exposure have been investigated using flow cytometry. Synchronous cell populations in G1, S and late-S G2/M phase were used, which were prepared by centrifugal elutriation. Cells were irradiated with particles from Z = 10 (neon) up to 96 (uranium) in the energy range from 2.4 to 17.4 MeV/u and the LET range from 415 to 16,225 keV/micron at the UNILAC at GSI, Darmstadt. For comparison, experiments with 250 kV X-rays were performed. For light particles like neon, cell cycle perturbations comparable with those after X-ray irradiation were found, and with increasing LET an increasing delay per particle traversal was observed. For the highest LET values extended delavy in G1, S and G2/M phase were detected immediately after irradiation. A large fraction of the cells remained in S or G2/M phase up to 48 h or longer after irradiation: they probably died in interphase. The prolongation of delays with increasing LET is in contrast with inactivation cross section measurements, where cross sections reach a plateau at LET values > 500 keV/micron. No significant cell age dependence of cycle delays was detected for the very high LET values. In addition to cell cycle delays, two effects related to the DNA content as determined by flow cytometry were found after irradiation with very high LET particles, that were attributed to cell fusion and drastic morphological changes of the cells. Estimations based on the dose deposited by a single particle hit in the cell nucleus and the actual number of hits show that the basic trend of the experimental results can be explained by the stochastic properties of particle radiation.

show abstract

“…The present model offers an efficient method to model such effects. Differences in biological response have been seen between hydrogen and helium ions at the same LET [27,28] and with low-energy heavy ions [29,30], but useful comparisons of fast and slow ions have not been made. For estimating the risk to astronauts from space radiation, differences in radiation quality for ions with the same LET may have important implications.…”

Section: Model For Radial Electron Spectrummentioning

confidence: 99%

Radial distribution of electron spectra from high-energy ions

Cucinotta

Katz

Wilson

1998

Radiation and Environmental Biophysics

View full text Add to dashboard Cite

The average track model describes the response of physical and biological systems using radial dose distribution as the key physical descriptor. We report on an extension of this model to describe the average distribution of electron spectra as a function of radial distance from an ion. We present calculations of these spectra for ions of identical linear energy transfer (LET), but dissimilar charge and velocity to evaluate the differences in electron spectra from these ions. To illustrate the usefulness of the radial electron spectra for describing effects that are not described by electron dose, we consider the evaluation of the indirect events in microdosimetric distributions for ions. We show that folding our average electron spectra model with experimentally determined frequency distributions for photons or electrons provides a good representation of radial event spectra from high-energy ions in 0.5-2 µm sites.

show abstract

Heavy Ion Effects on Cellular DNA: Strand Break Induction and Repair in Cultured Diploid Lens Epithelial Cells

Cited by 42 publications

References 14 publications

Distinct Roles of Ape1 Protein, an Enzyme Involved in DNA Repair, in High or Low Linear Energy Transfer Ionizing Radiation-induced Cell Killing

Distinct Roles of Ape1 Protein, an Enzyme Involved in DNA Repair, in High or Low Linear Energy Transfer Ionizing Radiation-induced Cell Killing

Cell Cycle Delays Induced by Heavy Ion Irradiation of Synchronous Mammalian Cells

Radial distribution of electron spectra from high-energy ions

Contact Info

Product

Resources

About