Heat-Transfer Resistance at Solid–Liquid Interfaces: A Tool for the Detection of Single-Nucleotide Polymorphisms in DNA

Grinsven, Bart van; Bon, Natalie Vanden; Strauven, H.; Grieten, Lars; Murib, Mohammed Sharif; Monroy, Kathia L. Jiménez; Janssens, Stoffel D.; Haenen, Ken; Schöning, Michael J.; Vermeeren, Veronique; Ameloot, Marcel; Michiels, Luc; Thoelen, Ronald; Ceuninck, W. De; Wagner, Patrick

doi:10.1021/nn300147e

Cited by 76 publications

(115 citation statements)

References 44 publications

Supporting

Mentioning

113

Contrasting

Order By: Relevance

“…Ranging from surfaceconductive field-effect transistors, 3,4 thermionic emission devices, [5][6][7] to the starting point for many functionalisation experiments leading to novel biosensor concepts. [8][9][10] In many of these situations, the diamond surface is in contact with a fluid. This can be pure water, but also buffer solutions or (aggressive) mixtures for electrochemical experiments are used.…”

Section: Introductionmentioning

confidence: 99%

Evidence for phase separation of ethanol-water mixtures at the hydrogen terminated nanocrystalline diamond surface

Janssens

Drijkoningen

Saitner

et al. 2012

The Journal of Chemical Physics

Self Cite

View full text Add to dashboard Cite

Interactions between ethanol-water mixtures and a hydrophobic hydrogen terminated nanocrystalline diamond surface, are investigated by sessile drop contact angle measurements. The surface free energy of the hydrophobic surface, obtained with pure liquids, differs strongly from values obtained by ethanol-water mixtures. Here, a model which explains this difference is presented. The model suggests that, due to a higher affinity of ethanol for the hydrophobic surface, when compared to water, a phase separation occurs when a mixture of both liquids is in contact with the H-terminated diamond surface. These results are supported by a computational study giving insight in the affinity and related interaction at the liquid-solid interface.

show abstract

Section: Introductionmentioning

confidence: 99%

Evidence for phase separation of ethanol-water mixtures at the hydrogen terminated nanocrystalline diamond surface

Janssens

Drijkoningen

Saitner

et al. 2012

The Journal of Chemical Physics

Self Cite

View full text Add to dashboard Cite

show abstract

“…Based on the difference in T m , this R th -based sensor was able to detect SNPs during thermal denaturation of short dsDNA fragments of the PAH gene. 24 In the current study, the R th -based sensor was evaluated for possible use in routine genetic analysis. Therefore, it would be advantageous if the R th method was applicable to longer, ideally exon-size, DNA fragments to simplify the development of a multiplex assay for screening an entire …”

Section: Discussionmentioning

confidence: 99%

“…The sensor setup was previously described. 24 Briefly, when performing a denaturation experiment, a dsDNA-functionalized NCD sample is attached with its backside to a copper (Cu) lid of the setup and connected with its functionalized front end to a flow cell, filled with 1× PBS buffer. The thermal readout of the setup consists of two thermocouples monitoring the temperature of both the Cu backside contact (T1) and the liquid compartment of the flow cell (T2).…”

Section: Sensor Setupmentioning

confidence: 99%

“…24 This technology is based on the change in heat-transfer resistance (R th ) of diamond sensor surfaces upon the thermal denaturation of immobilized dsDNA. The increase of R th is explained by the morphological changes of dsDNA during denaturation.…”

Section: Introductionmentioning

confidence: 99%

“…24,25 The probe DNA sequences were all modified at the 5′-end with an NH 2 -label and a spacer of seven adenosine (A) bases. To ensure that the NH 2 -label was free for immobilization, the probe DNA sequences were denatured for 5 minutes at 95°C before immobilization.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Heat-transfer-based detection of SNPs in the PAH gene of PKU patients

Bon¹,

Grinsven²,

Murib³

et al. 2014

IJN

Self Cite

View full text Add to dashboard Cite

Conventional neonatal diagnosis of phenylketonuria is based on the presence of abnormal levels of phenylalanine in the blood. However, for carrier detection and prenatal diagnosis, direct detection of disease-correlated mutations is needed. To speed up and simplify mutation screening in genes, new technologies are developed. In this study, a heat-transfer method is evaluated as a mutation-detection technology in entire exons of the phenylalanine hydroxylase (PAH) gene. This method is based on the change in heat-transfer resistance (R th ) upon thermal denaturation of dsDNA (double-stranded DNA) on nanocrystalline diamond. First, ssDNA (single-stranded DNA) fragments that span the size range of the PAH exons were successfully immobilized on nanocrystalline diamond. Next, it was studied whether an R th change could be observed during the thermal denaturation of these DNA fragments after hybridization to their complementary counterpart. A clear R th shift during the denaturation of exon 5, exon 9, and exon 12 dsDNA was observed, corresponding to lengths of up to 123 bp. Finally, R th was shown to detect prevalent single-nucleotide polymorphisms, c.473G>A (R158Q), c.932T>C (p.L311P), and c.1222C>T (R408W), correlated with phenylketonuria, displaying an effect related to the different melting temperatures of homoduplexes and heteroduplexes.

show abstract

Synchronized, Spontaneous, and Oscillatory Detachment of Eukaryotic Cells: A New Tool for Cell Characterization and Identification

et al. 2022

Self Cite

View full text Add to dashboard Cite

Despite the importance of cell characterization and identification for diagnostic and therapeutic applications, developing fast and label-free methods without (bio)-chemical markers or surface-engineered receptors remains challenging. Here, we exploit the natural cellular response to mild thermal stimuli and propose a label-and receptor-free method for fast and facile cell characterization. Cell suspensions in a dedicated sensor are exposed to a temperature gradient, which stimulates synchronized and spontaneous cell-detachment with sharply defined time-patterns, a phenomenon unknown from literature. These patterns depend on metabolic activity (controlled through temperature, nutrients, and drugs) and provide a library of cell-type-specific indicators, allowing to distinguish several yeast strains as well as cancer cells. Under specific conditions, synchronized glycolytic-type oscillations are observed during detachment of mammalian and yeast-cell ensembles, providing additional cell-specific signatures. These findings suggest potential applications for cell viability analysis and for assessing the collective response of cancer cells to drugs.

show abstract

Heat-Transfer Resistance at Solid–Liquid Interfaces: A Tool for the Detection of Single-Nucleotide Polymorphisms in DNA

Cited by 76 publications

References 44 publications

Evidence for phase separation of ethanol-water mixtures at the hydrogen terminated nanocrystalline diamond surface

Evidence for phase separation of ethanol-water mixtures at the hydrogen terminated nanocrystalline diamond surface

Heat-transfer-based detection of SNPs in the PAH gene of PKU patients

Synchronized, Spontaneous, and Oscillatory Detachment of Eukaryotic Cells: A New Tool for Cell Characterization and Identification

Contact Info

Product

Resources

About